cDNA Clone FAM83D

• Plasmid: pGEX6P1 FAM83D
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C FAM83D
• Genbank: NM_030919.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGA
CTGCACGCCGGTTTTTGTCCGAGGGCTGTCGAGTCCGAGCGCCGCCATGG
CTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCG
TGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGC
TCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGC
GACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATT
CTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGC
GGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACT
TCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCC
GCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTT
CCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTC
TGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGAC
GTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAA
ACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAAT
TTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTA
ATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAAC
TAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCC
GCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAAC
AGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATCT
GGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCC
TGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAA
CCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGC
TAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCAGAGATGCCCG
CAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGTT
AGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAA
GGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGC
TGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCACAGCATGTGCT
GGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGAT
TTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCT
TTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTCAAAAATGTCT
GTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAGG
CTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACT
TCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCACCTGGAACTG
TACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGCAATTCCACTT
CGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGA
GAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGC
AGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATCCTTCCTATCA
GTAAGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMARACLIQRLPIKRDCTPV
FVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEEL
VAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDS
FGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPRG
AGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVA
VYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIG
KVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRI
LYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSS
TPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDA
ATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSRS
TTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVAS
STGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIR
SRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28270
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can excise BamHI-Not or BamHI-Sal1. Silent change at Glu316 (GAG > GAA)
• Low Temp: No