cDNA Clone FAM83D

• Plasmid: pcDNA5 FRT/TO-GFP-FAM83D F313A F317A-HA
• Parent Plasmid: pcDNA5 FRT-TO-GFP
• Expressed: GFP-FAM83D F313A F317A-HA
• Genbank: NM_030919.2
• Tag: HA
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGACTGCACGCCGGTTTTTGTCCGAGGGCTGTCGAGTCCGAGCGCCGCCATGG
CTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGC
TCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATT
CTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACT
TCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTT
CCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGAC
GTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAAT
TTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAAC
TAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAAC
AGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACgctGATCTGGAGgccCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCC
TGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGC
TAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCAGAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGTT
AGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGC
TGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCACAGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGAT
TTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTCAAAAATGTCT
GTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACT
TCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCACCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGCAATTCCACTT
CGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGC
AGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATCCTTCCTATCAGgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARACLIQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTE
LFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYR
GATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITG
NIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILSGQVVEHADLEARILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTL
GNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVIT
RIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRN
LNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28250
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Homo sapiens family with sequence similarity 83 member D (FAM83D)* ; Phe313 and Phe317 > Ala. These numbers equate to Phe283/287 in FAM83D Q9H4H8.3 N-terminally truncated variant). Parental 41455