cDNA Clone OSR1

• Plasmid: pet15 6HIS SUMO OSR1 wt FL 1-527
• Parent Plasmid: pET15 6HIS SUMO
• Expressed: 6*HIS-(SUMO)-OSR1 wt FL (1-527)
• Genbank: NP_005100
• Tag: 6His
• Tag: SUMO

Sequence of Insert

GGATCCATGTCCGAAGACTCGAGCGCCCTGCCCTGGTCCATCAACAGGGACGATTACGAGCTGCAGGAGGTGATCGGGAGTGGAGCAACTGCTGTAGTCCAAGCAGCTTATTGTGCCCCTAAAAAGGAGAAAGTGGCAATCAAACGGATAAACCTTGAGAAATGTCAAACTAGCATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGTCAATGCCATCATCCTAATATTGTATCTTACTACACATCTTTTGTGGTAAAAGATGAGCTGTGGCTTGTCATGAAGCTGCTAAGTGGAGGTTCTGTTCTGGATATTATTAAGCACATTGTGGCAAAAGGGGAACACAAAAGTGGAGTCCTAGATGAATCTACCATTGCTACGATACTCCGAGAAGTACTGGAAGGGCTGGAATATCTGCATAAAAATGGACAGATCCACAGAGATGTGAAAGCTGGAAACATTCTTCTTGGAGAAGATGGCTCAGTACAGATTGCAGACTTTGGGGTTAGTGCTTTTTTAGCAACTGGTGGTGATATTACCCGAAATAAAGTGAGAAAGACCTTTGTTGGCACCCCTTGTTGGATGGCACCTGAAGTTATGGAACAGGTCCGTGGTTATGATTTCAAAGCTGATATTTGGAGTTTTGGAATTACAGCAATTGAATTGGCTACAGGGGCGGCTCCTTATCATAAATATCCACCAATGAAGGTTTTAATGCTGACACTGCAGAACGATCCTCCTTCTTTGGAAACTGGTGTTCAAGATAAAGAAATGCTGAAAAAATATGGAAAATCATTTAGAAAAATGATTTCATTGTGCCTTCAAAAAGATCCAGAAAAAAGACCAACAGCAGCAGAACTATTAAGGCACAAATTTTTCCAGAAAGCAAAGAATAAAGAATTTCTTCAAGAAAAAACATTGCAGAGAGCACCAACCATTTCTGAAAGAGCAAAAAAGGTTCGGAGGGTACCAGGTTCCAGTGGGCGTCTTCATAAGACAGAGGATGGAGGCTGGGAGTGGAGTGATGATGAATTTGATGAAGAAAGTGAGGAAGGGAAAGCAGCAATTTCACAACTCAGGTCTCCCCGAGTGAAAGAATCAATATCAAATTCTGAGCTCTTTCCAACAACTGATCCTGTGGGTACTTTGCTCCAAGTTCCAGAACAGATCTCTGCTCATCTACCTCAGCCAGCTGGGCAGATTGCTACACAGCCAACTCAAGTCTCTCTCCCACCCACCGCAGAGCCAGCAAAAACAGCTCAGGCTTTGTCTTCAGGATCAGGTTCACAAGAAACCAAGATCCCAATCAGTCTAGTACTAAGATTAAGGAATTCCAAAAAAGAACTAAATGATATTCGATTTGAATTTACTCCTGGGAGAGATACAGCAGAGGGTGTCTCTCAGGAACTCATTTCTGCTGGCCTGGTCGACGGAAGGGATTTAGTAATAGTGGCAGCTAATTTGCAGAAAATTGTGGAAGAACCTCAGTCAAATCGATCTGTCACTTTCAAACTGGCGTCTGGTGTCGAAGGCTCAGATATTCCTGATGATGGTAAACTGATAGGATTTGCCCAGCTCAGCATCAGCTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please put from GST (DU6099) into both 6*HIS (only) and 6*HIS-SUMO bacterial expression vector.