cDNA Clone SELO

• Plasmid: pMEX3C SELO 125-end D348A
• Parent Plasmid: pMEX3C
• Expressed: MBP-SELO 125-end D348A
• Genbank: BC110866.1
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCTGCCACCATGAGCGGCAACGCGCTCCTGCCGGGCGCCGAGCCCGCCGCGCACTGCTACTGCGGCCACCAATTCGGCCAGTTCGCCGGGCAGCTGG
GCGACGGCGCCGCCATGTACCTGGGCGAGGTGTGCACGGCGAACGGCGAGCGCTGGGAGCTGCAGCTCAAGGGCGCCGGGCCCACGCCCTTCTCCAGACA
GGCCGACGGTCGCAAGGTCCTACGGTCAAGCATCCGGGAGTTTCTATGCAGCGAAGCCATGTTCCACCTGGGAGTCCCCACCACACGGGCCGGCGCCTGC
GTCACGTCCGAGTCCACGGTGGTGCGCGACGTGTTCTATGATGGTAATCCCAAATATGAACAATGCACGGTTGTGTTGCGTGTAGCTTCCACTTTCATAA
GGTTTGGATCCTTTGAGATTTTTAAGTCTGCAGATGAGCACACAGGGCGTGCAGGCCCCAGCGTGGGGAGGAACGACATTCGAGTGCAGCTGCTCGACTA
TGTCATCAGCTCCTTTTACCCCGAGATCCAGGCTGCTCATGCCAGCGACAGCGTGCAGAGAAATGCTGCCTTCTTCCGGGAGGTGACGCGGCGCACGGCG
CGGATGGTGGCCGAGTGGCAGTGTGTGGGCTTCTGCCACGGCGTGCTCAACACCGACAACATGAGCATCCTGGGGCTCACCATCGCCTACGGGCCCTTTG
GCTTCCTGGACAGGTACGACCCCGACCACGTGTGCAATGCCTCCGACAACACCGGCCGCTACGCGTACAGCAAGCAGCCCGAGGTGTGCAGGTGGAACCT
GCGGAAGCTGGCCGAGGCCCTGCAGCCGGAACTGCCCCTGGAGCTGGGGGAGGCCATCCTGGCCGAGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTG
CAGAAGATGCGCAGGAAGCTGGGCCTCGTGCAGGTGGAGCTGGAGGAAGACGGGGCGCTGGTGTCCAAGCTCCTGGAGACCATGCATCTGACCGGTGCCG
ACTTCACAAACACCTTCTACTTGCTGAGCTCCTTCCCAGTGGAGCTAGAGTCGCCAGGCCTGGCGGAATTCCTGGCCAGGCTGATGGAGCAGTGTGCCTC
CCTGGAGGAGCTGAGGCTGGCCTTCCGGCCCCAGATGGATCCCCGGCAGCTATCCATGATGCTGATGCTGGCGCAGTCAAACCCGCAGCTGTTCGCGCTT
ATGGGCACCCGGGCAGGCATCGCCAGGGAGCTGGAGCGTGTGGAGCAGCAGTCTCGGCTGGAGCAGCTGAGTGCGGCAGAGCTGCAGAGCAGGAACCAGG
GCCACTGGGCTGACTGGCTACAGGCGTACAGAGCCCGGCTGGACAAGGACCTGGAAGGCGCTGGGGACGCTGCCGCCTGGCAGGCTGAGCACGTGCGCGT
GATGCACGCCAACAACCCGAAGTACGTGCTGAGGAACTACATCGCGCAGAATGCCATCGAGGCTGCCGAGCGCGGGGACTTCTCAGAGGTGCGGCGGGTG
CTGAAACTACTGGAGACCCCTTACCACTGCGAGGCGGGGGCCGCCACAGACGCCGAGGCCACGGAAGCCGACGGGGCGGACGGCAGGCAGCGCTCCTACA
GCAGTAAGCCCCCGCTCTGGGCAGCAGAACTGTGCGTGACATGATCTTCGTAAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSATMSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTANGERWELQLKGAGPTPFSRQADGRKVLRSSIREFLCSEAMFHLGVPTTRAG
ACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFREVTRR
TARMVAEWQCVGFCHGVLNTDNMSILGLTIAYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLELGEAILAEEFDAEFQRH
YLQKMRRKLGLVQVELEEDGALVSKLLETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPRQLSMMLMLAQSNPQLF
ALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVR
RVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT*SS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51671
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SELO has a stop codon 3aa from the actual stop which appears to be read through. Lee et al. Characterization of mammalian Selenoprtein O: A Redox-active mitochondrial protein. PLos One 9(4): e95518. doi:10.1371/journal.pone.0095518