cDNA Clone MCM6 (mouse)

• Plasmid: pGEX6P1 MCM6 (mouse) 601-821
• Parent Plasmid: pGEX6P1
• Expressed: GST MCM6 (mouse) 601-821
• Genbank: NP_032593.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCCTCCGCCAGAGGGATGGCTCTGGGGTCACCAAGTCCTCGTGGAGAATCACCGTGCGACAGCTTGAGAGCATGATCCGACTCTCAGAATCAATGG
CCCGCATGCACTGCTGTGACGAGGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATAAATCAATCATCCGTGTAGAGACGCCTGATGTCAA
TCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGATGAGGGACAAGGAGGTGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGC
TCCAGTGAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCTCGAACCTCATCGTGCTCCACCTCA
GGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGAGCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGA
GCTCATAAATAAAAAGACAATCATCGAGAAGGTTGTTCATCGTCTCACACACTACGATCACGTTCTGATTGAGCTCACCCAGGCTGGGCTGAAAGGCTCC
AGTGAAGGAAGCGAGAGCTATGAGGAAGACCCCTACCTGGTAGTCAACCCTAACTACTTGCTTGAAGATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLRQRDGSGVTKSSWRITVRQLESMIRLSESMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQE
EEIQMETDEGQGGVNGHADSPAPVNRFNGSSEDASQETVSKPSLRLGFAEYCRISNLIVLHLRKMEEEEDESALKRSELVNWYLKEIESEIDSEEELINK
KTIIEKVVHRLTHYDHVLIELTQAGLKGSSEGSESYEEDPYLVVNPNYLLED*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51793
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of M. musculus Mcm6 (600-821), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.