cDNA Clone CUL2

• Plasmid: pGEX6P1 CUL2 (mouse) 1-222
• Parent Plasmid: pGEX6P1
• Expressed: GST CUL2 (mouse) 1-222
• Genbank: NP_083678.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTGTTGTTATGTTGGAATATGTCGAGC
GAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTT
GGAAAGTCACGTTCGGCATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAAGGCGCC
GACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAAATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGCTTCG
AGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTT
CCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCCTTTCTGACTGAAACGGGAGAATATTACAAGCAAGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHV
RHLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSPFLTETGEYYKQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51794
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of M. musculus Cullin2 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.