cDNA Clone CDC48 (mouse)

• Synonyms: p97
• Plasmid: pGEX6P1 CDC48 (mouse) 1-180
• Parent Plasmid: pGEX6P1
• Expressed: GST CDC48 (mouse) 1-180
• Genbank: NP_033529.3
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGCCTCTGGAGCCGATTCAAAAGGTGATGATTTATCAACAGCCATTCTCAAACAGAAGAACCGACCCAATCGGTTAATTGTTGATGAAGCCA
TCAATGAAGATAACAGCGTGGTGTCCTTGTCCCAGCCCAAGATGGATGAACTGCAGTTGTTCCGAGGTGACACGGTGTTGCTAAAAGGAAAGAAAAGACG
GGAAGCTGTATGCATTGTTCTTTCTGATGACACGTGTTCTGATGAGAAGATTCGAATGAATAGAGTTGTTCGGAATAACCTCCGAGTTCGCCTAGGAGAT
GTCATCAGCATCCAGCCATGCCCTGATGTAAAGTATGGCAAACGTATCCACGTTCTACCCATCGATGACACAGTGGAAGGCATCACTGGCAATCTCTTTG
AGGTATACCTTAAGCCGTACTTCCTGGAAGCTTATCGGCCCATCCGTAAAGGAGATATTTTTCTTGTCCGGGGTGGGATGCGTGCTGTGGAGTTCAAAGT
TGTAGAGACAGATCCCAGCCCTTACTGTATTGTTGCTCCAGACACATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVC
IVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETD
PSPYCIVAPDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to express M. musculus Cdc48/p97 (1-180), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.