cDNA Clone KIAA1164

• Plasmid: pGEX6P1 KIAA1164 C266A
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1164 C266A
• Genbank: NM_001040450.1 GI:94721333
• Tag: GST

Sequence of Insert

AGATCTATGGAGAGCAGCCCCGAGAGCCTGCAGCCGCTAGAACACGGGGTGGCGGCCGGGCCAGCGTCAGGGACAGGTTCTTCGCAGGAAGGGCTACAGGAGACCAGGCTCGCCGCTGGTGATGGTCCTGGGGTATGGGCGGCGGAGACCAGCGGCGGGAATGGGCTGGGGGCGGCGGCCGCCAGGAGGAGCCTCCCGGACTCGGCTTCTCCCGCGGGCTCTCCTGAGGTTCCCGGACCCTGCAGCTCCTCCGCGGGTTTGGACTTGAAGGACAGTGGTTTGGAGAGTCCTGCTGCCGCCGAGGCGCCTCTGAGAGGGCAGTACAAGGTGACCGCCTCCCCGGAGACAGCCGTGGCCGGAGTGGGTCATGAGTTGGGTACCGCCGGAGACGCGGGAGCCCGCCCGGATCTCGCCGGCACCTGCCAAGCAGAACTGACCGCCGCCGGCTCCGAAGAGCCCAGCAGCGCCGGCGGCCTCAGCAGCAGTTGCAGCGACCCGAGCCCTCCTGGGGAATCTCCGAGCCTGGACTCTCTGGAGTCGTTCTCTAACCTGCATTCTTTTCCCAGTAGCTGCGAGTTCAATAGTGAGGAGGGAGCGGAGAACAGGGTCCCTGAGGAGGAGGAGGGCGCGGCGGTGTTGCCCGGGGCTGTTCCTCTGTGCAAGGAGGAGGAGGGGGAGGAGACCGCTCAGGTGCTGGCGGCCTCCAAGGAACGCTTCCCGGGACAATCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCGCTCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAAGATTCACTGGTGTTCGAGTGTTTGAATATACACCAGAATGCATAGTATTTGATCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATACAAAGGACAACAAGATCAGATAGATCAGGATTATCTTATGGCATTATCTCTACAACAAGAACAGCAGAGCCAAGAGATCAATTGGGAACAAATCCCGGAAGGAATCAGTGATTTGGAACTAGCAAAGAAACTCCAAGAGGAAGAGGACAGACGGGCTTCTCAATACTATCAGGAACAGGAACAAGCAGCAGCTGCTGCTGCTGCTGCTTCTACACAGGCTCAGCAGGGCCAGCCAGCACAAGCCTCTCCATCAAGTGGAAGACAATCTGGGAATAGTGAACGTAAACGGAAGGAACCACGAGAAAAAGATAAAGAAAAAGAAAAGGAAAAAAATAGCTGTGTTATTTTGTAAATGGAGAGCAGCCCCGAGAGCCTGCAGCCGCTAGAACACGGGGTGGCGGCCGGGCCAGCGTCAGGGACAGGTTCTTCGCAGGAAGGGCTACAGGAGACCAGGCTCGCCGCTGGTGATGGTCCTGGGGTATGGGCGGCGGAGACCAGCGGCGGGAATGGGCTGGGGGCGGCGGCCGCCAGGAGGAGCCTCCCGGACTCGGCTTCTCCCGCGGGCTCTCCTGAGGTTCCCGGACCCTGCAGCTCCTCCGCGGGTTTGGACTTGAAGGACAGTGGTTTGGAGAGTCCTGCTGCCGCCGAGGCGCCTCTGAGAGGGCAGTACAAGGTGACCGCCTCCCCGGAGACAGCCGTGGCCGGAGTGGGTCATGAGTTGGGTACCGCCGGAGACGCGGGAGCCCGCCCGGATCTCGCCGGCACCTGCCAAGCAGAACTGACCGCCGCCGGCTCCGAAGAGCCCAGCAGCGCCGGCGGCCTCAGCAGCAGTTGCAGCGACCCGAGCCCTCCTGGGGAATCTCCGAGCCTGGACTCTCTGGAGTCGTTCTCTAACCTGCATTCTTTTCCCAGTAGCTGCGAGTTCAATAGTGAGGAGGGAGCGGAGAACAGGGTCCCTGAGGAGGAGGAGGGCGCGGCGGTGTTGCCCGGGGCTGTTCCTCTGTGCAAGGAGGAGGAGGGGGAGGAGACCGCTCAGGTGCTGGCGGCCTCCAAGGAACGCTTCCCGGGACAATCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCGCTCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAAGATTCACTGGTGTTCGAGTGTTTGAATATACACCAGAATGCATAGTATTTGATCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATACAAAGGACAACAAGATCAGATAGATCAGGATTATCTTATGGCATTATCTCTACAACAAGAACAGCAGAGCCAAGAGATCAATTGGGAACAAATCCCGGAAGGAATCAGTGATTTGGAACTAGCAAAGAAACTCCAAGAGGAAGAGGACAGACGGGCTTCTCAATACTATCAGGAACAGGAACAAGCAGCAGCTGCTGCTGCTGCTGCTTCTACACAGGCTCAGCAGGGCCAGCCAGCACAAGCCTCTCCATCAAGTGGAAGACAATCTGGGAATAGTGAACGTAAACGGAAGGAACCACGAGAAAAAGATAAAGAAAAAGAAAAGGAAAAAAATAGCTGTGTTATTTTGTAAGATCCCCGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESSPESLQPLEHGVAAGPASGTGSSQEGLQETRLAAGDGPGVWAAETSGGNGLGAAAARRSLPDSASPAGSPEVPGPCSSSAGLDLKDSGLESPAAAEAPLRGQYKVTASPETAVAGVGHELGTAGDAGARPDLAGTCQAELTAAGSEEPSSAGGLSSSCSDPSPPGESPSLDSLESFSNLHSFPSSCEFNSEEGAENRVPEEEEGAAVLPGAVPLCKEEEGEETAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPAPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAAAAAAAASTQAQQGQPAQASPSSGRQSGNSERKRKEPREKDKEKEKEKNSCVILZMESSPESLQPLEHGVAAGPASGTGSSQEGLQETRLAAGDGPGVWAAETSGGNGLGAAAARRSLPDSASPAGSPEVPGPCSSSAGLDLKDSGLESPAAAEAPLRGQYKVTASPETAVAGVGHELGTAGDAGARPDLAGTCQAELTAAGSEEPSSAGGLSSSCSDPSPPGESPSLDSLESFSNLHSFPSSCEFNSEEGAENRVPEEEEGAAVLPGAVPLCKEEEGEETAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPAPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAAAAAAAASTQAQQGQPAQASPSSGRQSGNSERKRKEPREKDKEKEKEKNSCVIL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55340
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate Cys 266 to Ala in 46765