cDNA Clone NCK2

• Plasmid: pcDNA5-FRT/TO-mCherry-NCK2
• Parent Plasmid: pcDNA5-FRT/TO-mCherry
• Expressed: mCherry-NCK2
• Genbank: NM_003581.4 → NP_003572.2
• Tag: mCherry
• Species: Human

Sequence of Insert

ggatccATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGC
CCAGCAGGACCAGGAGCTGGACATCAAGAAGAACGAGCGGCTGTGGTTGC
TGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAGGACG
GGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGG
CTCCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCA
GGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAG
TACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCC
GGCCTTCGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCC
TGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCGACGGTTGG
TGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGT
CTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCC
TGCGCAAGGGCGCCTCGCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCAT
GTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACCGAGGAGGAGCTCAA
CTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACC
CCGAGTGGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCC
AAAAACTACGTGGTGGTCCTCAGTGACGGGCCTGCCCTGCACCCTGCGCA
CGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTCGCGG
GCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCC
CTCAACGAGCGGGGCGTGGAGGGCGACTTCCTCATTAGGGACAGCGAGTC
CTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAAC
ACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGC
TTCCACACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTT
CACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGCAGtgag
cggccgc

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMTEEVIVIA
KWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERK
NSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRI
YDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGW
FPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSV
TEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGP
ALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFL
IRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHY
KKAPIFTSEHGEKLYLVRALQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28691
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cytoplasmic protein NCK2 isoform A. Parental 31558
• Low Temp: No