cDNA Clone ITSN1

• Synonyms: ITSN, SH3D1A, SH3P17
• Plasmid: pGEX6P-1-ITSN1
• Parent Plasmid: pGEX6P-1
• Expressed: GST-ITSN1
• Genbank: NM_001001132.1
• Tag: GST
• Species: Human

Sequence of Insert

CTCGAGATGGCTCAGTTTCCAACACCTTTTGGTGGCAGCCTGGATATCTGGGCCATAACTGTAGAGGAAAGAGCGAAGCATGATCAGCAGTTCCATAGTTTAAAGCCAATATCTGGATTCATTACTGGTGATCAAGCTAGAAACTTTTTTTTTCAATCTGGGTTACCTCAACCTGTTTTAGCACAGATATGGGCACTAGCTGACATGAATAATGATGGAAGAATGGATCAAGTGGAGTTTTCCATAGCTATGAAACTTATCAAACTGAAGCTACAAGGATATCAGCTACCCTCTGCACTTCCCCCTGTCATGAAACAGCAACCAGTTGCTATTTCTAGCGCACCAGCATTTGGTATGGGAGGTATCGCCAGCATGCCACCGCTTACAGCTGTTGCTCCAGTGCCAATGGGATCCATTCCAGTTGTTGGAATGTCTCCAACCCTAGTATCTTCTGTTCCCACAGCAGCTGTGCCCCCCCTGGCTAACGGGGCTCCCCCTGTTATACAACCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTTAGTAGATCTGGTCCAGGGTCACAACTAAACACTAAATTACAAAAGGCACAGTCATTTGATGTGGCCAGTGTCCCACCAGTGGCAGAGTGGGCTGTTCCTCAGTCATCAAGACTGAAATACAGGCAATTATTCAATAGTCATGACAAAACTATGAGTGGACACTTAACAGGTCCCCAAGCAAGAACTATTCTTATGCAGTCAAGTTTACCACAGGCTCAGCTGGCTTCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTTACAGCAGAGGAATTTATCCTGGCAATGCACCTCATTGATGTAGCTATGTCTGGCCAACCACTGCCACCTGTCCTGCCTCCAGAATACATTCCACCTTCTTTTAGAAGAGTTCGATCTGGCAGTGGTATATCTGTCATAAGCTCAACATCTGTAGATCAGAGGCTACCAGAGGAACCAGTTTTAGAAGATGAACAACAACAATTAGAAAAGAAATTACCTGTAACGTTTGAAGATAAGAAGCGGGAGAACTTTGAACGTGGCAACCTGGAACTGGAGAAACGAAGGCAAGCTCTCCTGGAACAGCAGCGCAAGGAGCAGGAGCGCCTGGCCCAGCTGGAGCGGGCGGAGCAGGAGAGGAAGGAGCGTGAGCGCCAGGAGCAAGAGCGCAAAAGACAACTGGAACTGGAGAAGCAACTGGAAAAGCAGCGGGAGCTAGAACGGCAGAGAGAGGAGGAGAGGAGGAAAGAAATTGAGAGGCGAGAGGCTGCAAAACGGGAACTTGAAAGGCAACGACAACTTGAGTGGGAACGGAATCGAAGGCAAGAACTACTAAATCAAAGAAACAAAGAACAAGAGGACATAGTTGTACTGAAAGCAAAGAAAAAGACTTTGGAATTTGAATTAGAAGCTCTAAATGATAAAAAGCATCAACTAGAAGGGAAACTTCAAGATATCAGATGTCGATTGACCACCCAAAGGCAAGAAATTGAGAGCACAAACAAATCTAGAGAGTTGAGAATTGCCGAAATCACCCATCTACAGCAACAATTACAGGAATCTCAGCAAATGCTTGGAAGACTTATTCCAGAAAAACAGATACTCAATGACCAATTAAAACAAGTTCAGCAGAACAGTTTGCACAGAGATTCACTTGTTACACTTAAAAGAGCCTTAGAAGCAAAAGAACTAGCTCGGCAGCACCTACGAGACCAACTGGATGAAGTGGAGAAAGAAACTAGATCAAAACTACAGGAGATTGATATTTTCAATAATCAGCTGAAGGAACTAAGAGAAATACACAATAAGCAACAACTCCAGAAGCAAAAGTCCATGGAGGCTGAACGACTGAAACAGAAAGAACAAGAACGAAAGATCATAGAATTAGAAAAACAAAAAGAAGAAGCCCAAAGACGAGCTCAGGAAAGGGACAAGCAGTGGCTGGAGCATGTGCAGCAGGAGGACGAGCATCAGAGACCAAGAAAACTCCACGAAGAGGAAAAACTGAAAAGGGAGGAGAGTGTCAAAAAGAAGGATGGCGAGGAAAAAGGCAAACAGGAAGCACAAGACAAGCTGGGTCGGCTTTTCCATCAACACCAAGAACCAGCTAAGCCAGCTGTCCAGGCACCCTGGTCCACTGCAGAAAAAGGTCCACTTACCATTTCTGCACAGGAAAATGTAAAAGTGGTGTATTACCGGGCACTGTACCCCTTTGAATCCAGAAGCCATGATGAAATCACTATCCAGCCAGGAGACATAGTCATGGTTAAAGGGGAATGGGTGGATGAAAGCCAAACTGGAGAACCCGGCTGGCTTGGAGGAGAATTAAAAGGAAAGACAGGGTGGTTCCCTGCAAACTATGCAGAGAAAATCCCAGAAAATGAGGTTCCCGCTCCAGTGAAACCAGTGACTGATTCAACATCTGCCCCTGCCCCCAAACTGGCCTTGCGTGAGACCCCCGCCCCTTTGGCAGTAACCTCTTCAGAGCCCTCCACGACCCCTAATAACTGGGCCGACTTCAGCTCCACGTGGCCCACCAGCACGAATGAGAAACCAGAAACGGATAACTGGGATGCATGGGCAGCCCAGCCCTCTCTCACCGTTCCAAGTGCCGGCCAGTTAAGGCAGAGGTCCGCCTTTACTCCAGCCACGGCCACTGGCTCCTCCCCGTCTCCTGTGCTAGGCCAGGGTGAAAAGGTGGAGGGGCTACAAGCTCAAGCCCTATATCCTTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAATGATGTCATCACCGTCCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGGTTCCCCAAGTCTTACGTGAAACTCATTTCAGGGCCCATAAGGAAGTCTACAAGCATGGATTCTGGTTCTTCAGAGAGTCCTGCTAGTCTAAAGCGAGTAGCCTCTCCAGCAGCCAAGCCGGTCGTTTCGGGAGAAGAATTTATTGCCATGTACACTTACGAGAGTTCTGAGCAAGGAGATTTAACCTTTCAGCAAGGGGATGTGATTTTGGTTACCAAGAAAGATGGTGACTGGTGGACAGGAACAGTGGGCGACAAGGCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAAGATTCAGAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAGGTTATTGCCTCATACACCGCCACCGGCCCCGAGCAGCTCACTCTCGCCCCTGGTCAGCTGATTTTGATCCGAAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAGCTGCAAGCACGTGGGAAAAAGCGCCAGATAGGCTGGTTCCCAGCTAATTATGTAAAGCTTCTAAGCCCTGGGACGAGCAAAATCACTCCAACAGAGCCACCTAAGTCAACAGCATTAGCGGCAGTGTGCCAGGTGATTGGGATGTACGACTACACCGCGCAGAATGACGATGAGCTGGCCTTCAACAAGGGCCAGATCATCAACGTCCTCAACAAGGAGGACCCTGACTGGTGGAAAGGAGAAGTCAATGGACAAGTGGGGCTCTTCCCATCCAATTATGTGAAGCTGACCACAGACATGGACCCAAGCCAGCAATGGTGTTCAGACTTACATCTCTTGGATATGTTGACCCCAACTGAAAGAAAGCGACAAGGATACATCCACGAGCTCATTGTCACCGAGGAGAACTATGTGAATGACCTGCAGCTGGTCACAGAGATTTTTCAAAAACCCCTGATGGAGTCTGAGCTGCTGACAGAAAAAGAGGTTGCTATGATTTTTGTGAACTGGAAGGAGCTGATTATGTGTAATATCAAACTACTAAAAGCGCTGAGAGTCCGCAAGAAGATGTCCGGGGAGAAGATGCCTGTGAAGATGATTGGAGACATCCTGAGCGCACAGCTGCCGCACATGCAGCCCTACATCCGCTTCTGCAGCCGCCAGCTCAACGGGGCTGCCCTGATCCAGCAGAAGACGGACGAGGCCCCAGACTTCAAGGAGTTCGTCAAAAGATTGGCAATGGATCCTCGGTGTAGAGGGATGCCACTCTCTAGTTTTATACTGAAGCCTATGCAACGGGTAACAAGATACCCACTGATCATTAAAAATATCCTGGAAAACACCCCTGAAAACCACCCGGACCACAGCCACTTGAAGCACGCCCTGGAGAAGGCGGAAGAGCTCTGTTCCCAGGTGAACGAAGGGGTGCGGGAGAAGGAGAACTCTGACCGGCTGGAGTGGATCCAGGCCCACGTGCAGTGTGAAGGCCTGTCTGAGCAACTTGTGTTCAATTCAGTGACCAATTGCTTGGGGCCGCGCAAATTTCTGCACAGTGGGAAGCTCTACAAGGCCAAGAGCAACAAGGAGCTGTATGGCTTCCTTTTCAACGACTTCCTCCTGCTGACTCAGATCACGAAGCCTTTGGGGTCTTCTGGCACCGACAAAGTCTTCAGCCCCAAATCAAACCTGCAGTATAAAATGTATAAAACACCTATTTTCCTAAATGAGGTTCTAGTAAAATTACCCACCGACCCTTCTGGAGACGAGCCCATCTTCCACATCTCCCACATTGACCGCGTCTATACTCTCCGAGCAGAAAGCATAAATGAAAGGACTGCCTGGGTGCAGAAAATCAAAGCTGCTTCTGAACTCTACATAGAGACTGAGAAAAAGAAGCGCGAGAAAGCGTACCTGGTCCGTTCCCAAAGGGCAACAGGCATTGGAAGGTTGATGGTGAACGTGGTTGAAGGCATCGAGTTGAAACCCTGTCGGTCACATGGAAAGAGCAACCCGTACTGTGAGGTGACCATGGGTTCCCAGTGCCACATCACCAAGACGATCCAGGACACTCTGAACCCCAAGTGGAATTCCAACTGCCAGTTCTTCATCCGAGACCTGGAGCAGGAAGTCCTCTGCATCACTGTGTTCGAGAGGGACCAGTTCTCACCAGATGATTTTTTGGGTCGGACGGAGATCCGTGTGGCGGACATCAAGAAAGACCAGGGCTCCAAAGGTCCAGTTACGAAGTGTCTTCTGCTGCACGAAGTCCCCACGGGAGAGATTGTGGTCCGCTTGGACCTGCAGTTGTTTGATGAGCCGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVEMAQFPTPFGGSLD
IWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWA
LADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAP
AFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAP
PVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVA
EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIW
NLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRS
GSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNL
ELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQL
EKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNK
EQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIEST
NKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRD
SLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREI
HNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEH
VQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQ
EPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQP
GDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPV
KPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNE
KPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKV
EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPK
SYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTY
ESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGS
GTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWE
GELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGM
YDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTT
DMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIF
QKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVK
MIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMD
PRCRGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKA
EELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKF
LHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQY
KMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWV
QKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRS
HGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITV
FERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRL
DLQLFDEP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28838
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: THis clone has 1 silent mutation D1348 and 1 naturally occuring SNP K1367R
• Low Temp: No