cDNA Clone HRAS

• Synonyms: C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV1, RASH1, p21ras, VHL
• Plasmid: pRETROX VHL aHRAS nano
• Parent Plasmid: pRetroX tight puro
• Expressed: VHL-5xGLY-aHRAS nanobody
• Species: Human

Sequence of Insert

VHL
GGATCCGCCATGGACTACAAGGACGATGACGATAAGGGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT

aASC
GAATTCGCCATGGGCAGCGTGAGCAGCGTGCCCACCAAGCTGGAGGTGGTGGCCGCCACCCCCACCAGCCTGCTGATCAGCTGGGACGCCCCCGCCGTGACCGTGGACTACTACGTGATCACCTACGGCGAGACCGGCGGCAACAGCCCCGTGCAGAAGTTCGAGGTGCCCGGCAGCAAGAGCACCGCCACCATCAGCGGCCTGAAGCCCGGCGTGGACTACACCATCACCGTGTACGCCTGGGGCTGGCACGGCCAGGTGTACTACTACATGGGCAGCCCCATCAGCATCAACTACAGGACCTGAGCGGCCGC

Amino Acid Sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVW
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMGSVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGGNSPVQKFEVPGSKSTATISGLKP
GVDYTITVYAWGWHGQVYYYMGSPISINYRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU57338
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-HRAS nanobody for use in degron work.
• Low Temp: No