cDNA Clone CLASPIN (Worm: Caenorhabditis elegans)

• Plasmid: pGEX6P1 Claspin C (elegans) 1-222
• Parent Plasmid: pGEX6P1
• Expressed: GST Claspin C (elegans) 1-222
• Genbank: NP_001251008.1
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAATTCTGATTCCTGTTCAACAAAAACCGTGTCAGCAGACGATACAGATTCTCCATCTTCTCTAGTTGCCGAACACACCATGTCCGATTCCC
AGGAGCAGCTGATATCTAGTGAAAACACGGAAGTCGATAAGCAAGCTGAAGAAGACCGAAAACTTCGTCGTCTTAATCGTCGTAAAGCACTGGCTTTAGA
ACGAAGTCCTCTTCCTGTTCCGAAGCTTGGGAGAGTCCTATTATCTGCCAATAGTACGATTGACCTGACAAATGAAGATGATGACCCAAGTAACAAATGG
TTCAAAAAGACGTTTAATGCTGAAAAAATTCCGAAATTGGCAAAAAGTCCGGAAGAAGCTAAATTATTCGCCGTTCCTCGAAAAATATCGAAGAACGCAC
CCAAGAGCCTTCTGACCAGCGAGACTTTAAAAAAGAAGCTTCAAGCTTCGATGTCAGCGAAGCGACGGAAAGAACAAGAGCAACGTCGGAAAATGTATGA
AGAGGATAATGAACATCTGAAGGCTGGCAGTGATGAAGAAGAGGAAGAAGATGTCGTTTTTAAAAAAAAGAAGGGAAAGAAACCTGTGAATGAAGAAGAA
TATAAAAGCGAAGATGATGAAGACTATGAACCTGAAAATAGCGGAGAAGAAGATTCAGATGAGAAGAATAATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSDSCSTKTVSADDTDSPSSLVAEHTMSDSQEQLISSENTEVDKQAEEDRKLRRLNRRKALALERSPL
PVPKLGRVLLSANSTIDLTNEDDDPSNKWFKKTFNAEKIPKLAKSPEEAKLFAVPRKISKNAPKSLLTSETLKKKLQASMSAKRRKEQEQRRKMYEEDNE
HLKAGSDEEEEEDVVFKKKKGKKPVNEEEYKSEDDEDYEPENSGEEDSDEKNN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56360
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of N-terminus of C.elegans CLASPIN (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.
• Low Temp: No