cDNA Clone HRAS

• Synonyms: C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV1, RASH1, p21ras
• Plasmid: pBABED P 3FLAG HALO aHRAS nanobody
• Parent Plasmid: pBabeD puro 3FLAG
• Expressed: 3FLAG-HALO-5xGLY-aHRAS nanobody
• Genbank: -
• Tag: FLAG
• Species: Human

Sequence of Insert

HALO
ggatccgccATGGCAGAAATCGGTACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGTCGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGACCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGTCCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCACGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGGTATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGCCCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCAGAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGAGATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTTCCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATACATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGTTCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGTGGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGAGATCGCGCGCTGGCTGTCTACGCTAGAGATTTCCGGCggtggaggcggaggt
aASC
GAATTCGCCATGGGCAGCGTGAGCAGCGTGCCCACCAAGCTGGAGGTGGTGGCCGCCACCCCCACCAGCCTGCTGATCAGCTGGGACGCCCCCGCCGTGACCGTGGACTACTACGTGATCACCTACGGCGAGACCGGCGGCAACAGCCCCGTGCAGAAGTTCGAGGTGCCCGGCAGCAAGAGCACCGCCACCATCAGCGGCCTGAAGCCCGGCGTGGACTACACCATCACCGTGTACGCCTGGGGCTGGCACGGCCAGGTGTACTACTACATGGGCAGCCCCATCAGCATCAACTACAGGACCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSAMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGGGGGGEFMGSVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGGNSPVQKFEVPGSKSTATISGLK
PGVDYTITVYAWGWHGQVYYYMGSPISINYRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU57462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-HRAS nanobody for use in degron work.
• Low Temp: No