cDNA Clone NCK2

• Plasmid: pcDNA5-FRT/TO-FLAG-NCK2 W39K W149K
• Parent Plasmid: pcDNA5-FRT/TO-FLAG
• Expressed: FLAG-NCK2 W39K W149K
• Genbank: NM_003581.4 → NP_003572.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGC
CCAGCAGGACCAGGAGCTGGACATCAAGAAGAACGAGCGGCTGTGGTTGC
TGGACGACTCCAAGACGTGGaaGCGGGTGAGGAACGCGGCCAACAGGACG
GGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGG
CTCCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCA
GGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAG
TACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCC
GGCCTTCGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCC
TGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCGACGGTTGG
aaGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGT
CTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCC
TGCGCAAGGGCGCCTCGCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCAT
GTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACCGAGGAGGAGCTCAA
CTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACC
CCGAGTGGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCC
AAAAACTACGTGGTGGTCCTCAGTGACGGGCCTGCCCTGCACCCTGCGCA
CGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTCGCGG
GCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCC
CTCAACGAGCGGGGCGTGGAGGGCGACTTCCTCATTAGGGACAGCGAGTC
CTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAAC
ACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGC
TTCCACACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTT
CACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGCAGtgag
cggccgc

Amino Acid Sequence

MDYKDDDDKGSMTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWK
RVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDA
SPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVT
VMEKCSDGWKRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLS
NGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKN
ARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGN
VTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVD
NVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28939
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cytoplasmic protein NCK2 isoform A.
• Low Temp: No