cDNA Clone AFADIN

• Synonyms: AF6; MLL-AF6; MLLT4; AFDN
• Plasmid: pcDNA5 FRT/TO 3FLAG Afadin (MLLT4) S216A
• Parent Plasmid: pcDNA5 FRT/TO 3FLAG
• Expressed: 3FLAG Afadin (MLLT4) S216A
• Genbank: Codon optimised XM_005266996.3
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCTGCTGGCGGAAGAGATGAGGAACGCAGAAAGCTGGCCGACATCATCCACCACTGGAACGCCAACAGACTGGACCTGTTCGAGATCAGCCAGCCTACCGAGGACCTGGAATTTCACGGCGTGATGCGGTTCTACTTCCAAGACAAGGCCGCTGGCAACTTCGCCACCAAGTGCATCAGAGTGTCCAGCACCGCCACCACACAGGACGTGATCGAAACACTGGCCGAGAAGTTCAGACCCGACATGCGGATGCTGAGCAGCCCCAAGTACAGCCTGTATGAGGTGCACGTGTCCGGCGAGAGAAGGCTGGACATCGATGAGAAGCCTCTGGTGGTGCAGCTGAACTGGAACAAGGACGACCGCGAGGGCAGATTCGTGCTGAAGAACGAGAACGACGCCATTCCTCCTAAGAAGGCCCAGAGCAACGGCCCCGAGAAGCAAGAGAAAGAAGGCGTGATCCAGAACTTCAAGCGGACCCTGTCCAAGAAAGAGAAAAAAGAGAAGAAGAAGCGCGAGAAAGAGGCCCTGAGACAGGCCAGCGACAAGGATGACAGACCCTTCCAGGGCGAAGATGTGGAAAACAGCAGACTGGCCGCCGAGGTGTACAAGGACATGCCCGAGACAAGCTTCACCCGGACCATCGCCAATCCCGAGGTGGTCATGAAGCGGCGGAGACAGCAGAAACTGGAAAAGCGGATGCAAGAGTTCAGAAGCAGCGACGGCAGACCTGATTCTGGCGGCACCCTGAGAATCTACGCCGACAGCCTGAAGCCTAACATCCCCTACAAGACCATCCTGCTGAGCACCACCGATCCTGCCGATTTTGCCGTGGCCGAGGCTCTCGAGAAGTACGGCCTGGAAAAAGAAAACCCCAAGGACTACTGTATCGCCCGCGTGATGTTGCCTCCTGGCGCTCAGCATTCTGATGAGAAGGGCGCCAAAGAGATCATCCTGGACGACGACGAGTGCCCTCTGCAGATCTTTAGAGAGTGGCCCTCCGACAAGGGCATCCTGGTGTTCCAGCTGAAGAGGCGGCCTCCTGATCACATCCCTAAAAAGACCAAGAAGCACCTCGAGGGCAAGACCCCTAAGGGCAAAGAAAGAGCCGACGGCAGCGGCTACGGAAGCACACTGCCTCCAGAGAAGCTGCCCTACCTGGTGGAACTGAGCCCCGGCAGAAGAAACCACTTCGCCTACTACAACTACCACACCTACGAGGACGGCTCCGACAGCAGAGACAAGCCCAAGCTGTACAGACTGCAGCTGAGCGTGACCGAAGTGGGCACCGAGAAGCTGGACGACAACAGCATCCAGCTGTTCGGCCCTGGAATCCAGCCTCACCACTGCGACCTGACCAACATGGATGGCGTGGTCACAGTGACCCCTAGATCCATGGACGCCGAGACATACGTGGAAGGCCAGCGGATCAGCGAGACAACCATGCTGCAGAGCGGCATGAAGGTGCAGTTTGGCGCCAGCCACGTGTTCAAGTTCGTGGACCCTAGCCAGGATCATGCCCTGGCCAAGAGATCTGTGGATGGCGGCCTGATGGTCAAGGGCCCTAGACACAAGCCTGGCATCGTGCAAGAGACAACCTTCGATCTCGGCGGCGATATCCACAGCGGAACAGCCCTGCCTACCAGCAAGAGCACCACAAGACTGGACAGCGACAGAGTGTCTAGCGCCAGCTCTACAGCCGAGAGAGGCATGGTCAAGCCCATGATCAGAGTGGAACAGCAGCCCGACTACCGGCGCCAAGAGTCTAGAACTCAGGATGCCTCTGGACCCGAGCTGATCCTGCCTGCCTCCATCGAGTTTAGAGAGAGCAGCGAGGACAGCTTCCTGTCCGCCATCATCAACTACACCAACAGCAGCACCGTGCACTTCAAGCTGAGCCCCACCTACGTGCTGTACATGGCCTGTAGATACGTGCTGAGCAACCAGTACAGGCCCGACATCAGCCCTACCGAGAGAACCCACAAAGTGATCGCCGTGGTCAACAAGATGGTGTCCATGATGGAAGGGGTCATCCAGAAGCAGAAGAATATCGCTGGCGCCCTGGCCTTTTGGATGGCCAATGCAAGCGAGCTGCTGAACTTCATCAAGCAGGACCGGGACCTGAGCCGGATCACACTGGATGCTCAGGATGTGCTGGCCCACCTGGTGCAGATGGCCTTCAAGTATCTGGTGCACTGCCTGCAGTCCGAGCTGAACAACTACATGCCCGCCTTTCTGGACGACCCTGAAGAGAACTCACTGCAGCGGCCCAAGATCGACGATGTGCTGCATACACTGACAGGCGCCATGAGCCTGCTGCGGAGATGCAGAGTTAATGCTGCCCTGACCATTCAGCTGTTCAGCCAGCTGTTTCACTTTATCAACATGTGGCTGTTCAACCGGCTGGTCACAGACCCTGATAGCGGCCTGTGTAGCCACTATTGGGGAGCCATCATTCGGCAGCAGCTGGGACACATTGAAGCCTGGGCCGAGAAACAGGGACTCGAGCTGGCTGCCGATTGCCACCTGTCTAGAATCGTGCAGGCCACCACTCTGCTGACCATGGATAAGTACGCCCCTGACGACATCCCCAACATCAACAGCACCTGTTTTAAGCTGAATAGCCTCCAGCTGCAGGCCCTGCTGCAGAACTATCACTGTGCCCCAGACGAGCCTTTCATCCCCACCGACCTGATCGAGAACGTCGTGACCGTGGCTGAGAACACCGCCGATGAACTGGCCAGATCCGATGGCAGAGAGGTGCAGCTCGAGGAAGATCCCGATCTGCAGCTGCCTTTCCTGCTGCCTGAGGATGGCTACAGCTGTGACGTCGTGCGGAATATCCCCAACGGCCTGCAAGAATTCCTGGACCCTCTGTGCCAGCGGGGCTTCTGCAGACTGATCCCTCACACAAGAAGCCCTGGCACCTGGACCATCTACTTCGAGGGCGCCGATTACGAGAGCCATCTGCTGAGAGAGAACACAGAGCTGGCCCAGCCTCTGAGAAAAGAGCCCGAGATCATCACCGTGACACTGAAGAAACAGAACGGCATGGGCCTGTCCATCGTGGCCGCTAAAGGTGCCGGACAGGATAAGCTGGGCATCTACGTGAAGTCCGTCGTGAAAGGCGGAGCCGCCGATGTTGATGGTAGACTGGCTGCTGGCGATCAGCTGCTTTCCGTGGATGGCAGATCTCTCGTGGGCCTCAGCCAAGAAAGGGCCGCAGAGCTGATGACCAGAACCAGCAGCGTTGTGACCCTGGAAGTGGCCAAACAGGGCGCAATCTATCACGGCCTGGCCACACTGCTGAACCAGCCATCTCCTATGATGCAGAGAATCAGCGATCGGAGAGGCAGCGGCAAGCCCAGACCTAAGTCTGAGGGCTTCGAGCTGTACAACAATAGCACCCAGAACGGCTCCCCAGAGAGCCCTCAACTTCCTTGGGCCGAGTACAGCGAGCCCAAAAAACTGCCCGGCGACGACCGGCTGATGAAGAACAGAGCTGACCACCGCAGCAGCCCTAACGTGGCAAATCAGCCTCCATCTCCTGGCGGCAAAAGCGCCTATGCCTCCGGAACAACCGCCAAGATCACCAGCGTGTCCACCGGCAATCTGTGCACCGAGGAACAGACCCCTCCACCTAGACCTGAGGCTTACCCCATTCCTACACAGACCTACACCAGAGAGTACTTCACATTCCCCGCCAGCAAGTCCCAGGACAGAATGGCCCCTCCACAGAACCAGTGGCCTAACTACGAAGAGAAGCCCCACATGCACACCGACAGCAACCACAGCTCTATCGCCATCCAGAGAGTGACCAGAAGCCAAGAGGAACTGAGAGAGGACAAGGCTTACCAGCTGGAAAGACACAGAATCGAAGCCGCCATGGACCGGAAGTCCGACTCCGACATGTGGATCAACCAGAGCAGCAGCCTGGACAGCTCCACCAGCTCTCAAGAACACCTGAACCACTCCTCCAAGAGCGTGACACCAGCCAGCACACTGACCAAGTCTGGACCTGGCAGATGGAAAACCCCTGCCGCCATTCCAGCTACACCTGTGGCTGTGTCTCAGCCCATCAGAACCGACCTTCCACCTCCTCCGCCTCCACCTCCAGTTCATTACGCCGGCGATTTCGACGGCATGAGCATGGATTTGCCCCTGCCACCTCCACCAAGCGCCAATCAGATTGGACTGCCTAGCGCTCAGGTGGCCGCAGCCGAACGAAGAAAGAGGGAAGAACACCAGCGGTGGTACGAGAAAGAAAAGGCCCGGCTGGAAGAGGAACGCGAGAGAAAGCGCAGAGAGCAAGAGCGGAAACTGGGCCAGATGCGGACCCAGTCTCTGAACCCAGCTCCTTTCAGCCCTCTGACAGCCCAGCAGATGAAGCCTGAGAAGCCCTCCACACTGCAAAGACCCCAAGAGACAGTGATCCGCGAGCTGCAGCCACAGCAGCAACCTAGAACCATCGAGCGGAGGGACCTGCAGTACATTACCGTGTCCAAAGAAGAACTGAGCAGCGGCGACTCTCTGAGCCCTGATCCTTGGAAGCGGGACGCCAAAGAAAAGCTCGAGAAGCAGCAGCAGATGCACATCGTGGACATGCTGTCTAAAGAGATCCAAGAACTGCAGAGCAAGCCTGACAGAAGCGCCGAGGAAAGCGACAGACTGCGGAAGCTGATGCTCGAGTGGCAGTTCCAGAAGAGACTGCAAGAGTCCAAGCAGAAGGACGAGGACGACGAGGAAGAAGAGGACGACGACGTCGACACCATGCTGATCATGCAGAGACTCGAGGCTGAGCGGAGAGCCAGACTCCAAGACGAAGAACGTCGCAGACAGCAGCAGCTTGAGGAAATGCGCAAGAGAGAGGCCGAGGACCGGGCCAGACAAGAGGAAGAAAGACGGCGGCAAGAAGAAGAGAGGACCAAGAGGGACGCCGAAGAGAAGCGCCGACAAGAAGAGGGCTACTACAGCAGGCTGGAAGCCGAGCGTAGAAGGCAGCATGATGAGGCCGCTCGGAGACTGCTGGAACCTGAAGCACCAGGACTGTGCAGACCTCCTCTGCCTAGAGACTACGAGCCACCTAGTCCTTCTCCAGCACCTGGCGCACCACCACCACCTCCACAGAGAAATGCCAGCTACCTGAAAACTCAGGTGCTGAGCCCAGATAGCCTGTTCACCGCCAAGTTTGTGGCCTACAACGAGGAAGAGGAAGAAGAAGATTGCAGCCTGGCTGGCCCCAACAGCTATCCTGGATCTACTGGTGCTGCCGTGGGAGCCCACGATGCTTGCAGAGATGCTAAAGAGAAACGGTCCAAGAGCCAGGACGCCGACTCTCCAGGATCTTCTGGCGCCCCAGAGAACCTGACCTTTAAAGAGCGGCAGAGACTGTTCTCCCAGGGCCAAGACGTGTCCAACAAAGTGAAGGCCTCTAGGAAGCTGACCGAGCTGGAAAACGAGCTGAATACCAAGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDV
IETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKK
REKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTIANPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTT
DPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERAD
GSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETY
VEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGM
VKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMV
SMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMS
LLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCF
KLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHT
RSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQ
ERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHR
SSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQR
VTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPP
PVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQ
RPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQ
ESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEA
ARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKR
SKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No