cDNA Clone NEK10

• Plasmid: pcDNA5D FRT/TO GFP Nek10 tv 2 (long)
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP NEK10 tv 2 (long)
• Genbank: NM_152534.4 with 5 silent changes
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCTGATCAAGATAAAAAGGTGAAGACCACAGAAAAATCAACTGATAAACAGCAAGAAATCACCATCAGGGACTATTCAGATCTTAAAAGACTTCGGTGCCTTTTGAACGTCCAATCAAGCAAACAACAGCTTCCAGCCATTAACTTCGATAGTGCCCAAAATAGCATGACGAAGTCTGAGCCCGCCATCAGGGCGGGTGGACACAGAGCTCGGGGTCAGTGGCATGAATCCACAGAAGCTGTTGAACTTGAAAATTTTAGTATAAACTACAAGAATGAGAGAAATTTCAGCAAACATCCTCAGCGTAAACTATTTCAGGAGATCTTTACCGCCTTGGTGAAAAATAGACTCATAAGCAGAGAGTGGGTTAATCGAGCCCCATCTATTCATTTTCTGAGAGTGTTAATCTGTCTGAGGCTACTAATGAGGGACCCATGTTATCAGGAAATACTCCATAGCTTGGGTGGGATTGAAAACCTAGCTCAGTATATGGAGATTGTAGCCAATGAGTACCTCGGCTATGGAGAAGAGCAGCACACTGTGGACAAGCTGGTCAACATGACATATATTTTTCAAAAACTTGCTGCAGTCAAAGATCAAAGAGAATGGGTCACCACAAGTGGAGCCCACAAGACATTAGTAAATTTACTTGGTGCCCGAGATACTAATGTTCTATTGGGTTCCCTTCTGGCTCTGGCTAGTTTAGCAGAAAGTCAAGAATGTAGGGAGAAGATAAGTGAACTCAACATTGTAGAAAATCTGTTGATGATTTTACATGAATATGACTTGCTTTCTAAAAGACTAACAGCGGAGTTGCTGCGCCTACTTTGTGCAGAGCCCCAGGTGAAAGAGCAGGTGAAGCTCTATGAGGGGATACCGGTCCTCCTCAGTCTGCTCCACTCTGACCACTTGAAGCTCCTCTGGAGCATTGTCTGGATTCTGGTACAGGTTTGTGAGGACCCTGAGACCAGCGTGGAAATTCGCATTTGGGGAGGCATCAAACAGCTTCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGATCACTCCTCCATTGGAAGCCTGTCCAGTGCAAATGCTGCAGGCCGAATCCAGCAGCTTCATTTATCAGAAGACTTGAGCCCTAGGGAAATACAAGAAAATACTTTCTCACTTCAAGCAGCCTGCTGTGCTGCCCTCACTGAGCTGGTGCTCAATGACACCAATGCCCACCAGGTGGTTCAGGAAAATGGTGTATATACAATAGCAAAATTAATTTTACCAAATAAGCAAAAGAATGCAGCAAAAAGTAATCTATTACAGTGTTATGCTTTCAGAGCCTTGAGATTTCTCTTCAGTATGGAAAGAAACAGACCACTCTTTAAAAGACTTTTCCCCACAGACTTGTTTGAGATCTTCATTGACATAGGGCATTATGTACGTGATATCAGTGCTTATGAAGAATTGGTATCCAAGCTGAATTTATTAGTGGAGGATGAACTGAAGCAAATTGCTGAAAATATTGAAAGCATTAATCAGAACAAAGCTCCTTTGAAATATATAGGCAACTATGCAATTTTGGATCATCTTGGAAGTGGAGCTTTTGGCTGTGTTTACAAGGTTAGAAAGCATAGTGGTCAAAATCTTTTAGCAATGAAAGAGGTCAATTTACATAACCCAGCATTTGGGAAGGATAAGAAAGATCGAGACAGCAGCGTAAGGAATATTGTTTCTGAATTAACAATAATTAAAGAGCAGCTTTATCATCCCAACATTGTACGTTATTACAAAACATTTCTGGAAAATGATAGGTTGTACATAGTTATGGAGCTGATAGAAGGAGCCCCGCTTGGAGAGCATTTCAGTTCTTTGAAGGAAAAACATCACCATTTTACTGAAGAAAGACTATGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGATACTTACACAAGGAGAAGAGGATTGTCCATAGAGATCTGACACCAAACAACATTATGTTGGGGGATAAGGACAAAGTAACCGTTACTGACTTTGGCCTGGCAAAGCAAAAACAAGAAAACAGTAAACTCACGTCTGTGGTTGGAACAATCCTGTATTCTTGCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATGTCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCTGTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTAACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGATGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCTAGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCATGAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCAGCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGACGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACATATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAGAATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGACTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCACCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCCTTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGGGCCACAGATGGGCACATTCTTGTGGCAAGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGTGCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTTCCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGCAGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCCCAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGACCCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTGAAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGACCAAGAATCACCCAACCAAAAGATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNS
MTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLG
GIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLL
MILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGS
LSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLF
KRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPA
FGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDL
TPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSE
KVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSE
LSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRP
ALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNL
KRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSN
RYHSYPWGTKNHPTKR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58925
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Nucleotides 1-2090 of tv 2 amplified from RNA using RT14929/14930. Internal BamHI site killed using RT14927/14928. Insert cloned into BamHI site of DU58430. Junction at 3' BamHI site corrected using RT14931/14932. Missing insertion added using RT15012/15013.
• Low Temp: No