cDNA Clone NEK10

• Plasmid: pcDNA5D FRT/TO GFP NLS Nek10 tv 2 (long)
• Parent Plasmid: pcDNA5D FRT/TO GFP NLS
• Expressed: GFP NLS NEK10 tv 2 (long)
• Genbank: NM_152534.4 with 5 silent changes
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCTGATCAAGATAAAAAGGTGAAGACCACAGAAAAATCAACTGATAAACAGCAAGAAATCACCATCAGGGACTATTCAGATCTTAAAAGACTTCGGTGCCTTTTGAACGTCCAATCAAGCAAACAACAGCTTCCAGCCATTAACTTCGATAGTGCCCAAAATAGCATGACGAAGTCTGAGCCCGCCATCAGGGCGGGTGGACACAGAGCTCGGGGTCAGTGGCATGAATCCACAGAAGCTGTTGAACTTGAAAATTTTAGTATAAACTACAAGAATGAGAGAAATTTCAGCAAACATCCTCAGCGTAAACTATTTCAGGAGATCTTTACCGCCTTGGTGAAAAATAGACTCATAAGCAGAGAGTGGGTTAATCGAGCCCCATCTATTCATTTTCTGAGAGTGTTAATCTGTCTGAGGCTACTAATGAGGGACCCATGTTATCAGGAAATACTCCATAGCTTGGGTGGGATTGAAAACCTAGCTCAGTATATGGAGATTGTAGCCAATGAGTACCTCGGCTATGGAGAAGAGCAGCACACTGTGGACAAGCTGGTCAACATGACATATATTTTTCAAAAACTTGCTGCAGTCAAAGATCAAAGAGAATGGGTCACCACAAGTGGAGCCCACAAGACATTAGTAAATTTACTTGGTGCCCGAGATACTAATGTTCTATTGGGTTCCCTTCTGGCTCTGGCTAGTTTAGCAGAAAGTCAAGAATGTAGGGAGAAGATAAGTGAACTCAACATTGTAGAAAATCTGTTGATGATTTTACATGAATATGACTTGCTTTCTAAAAGACTAACAGCGGAGTTGCTGCGCCTACTTTGTGCAGAGCCCCAGGTGAAAGAGCAGGTGAAGCTCTATGAGGGGATACCGGTCCTCCTCAGTCTGCTCCACTCTGACCACTTGAAGCTCCTCTGGAGCATTGTCTGGATTCTGGTACAGGTTTGTGAGGACCCTGAGACCAGCGTGGAAATTCGCATTTGGGGAGGCATCAAACAGCTTCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGATCACTCCTCCATTGGAAGCCTGTCCAGTGCAAATGCTGCAGGCCGAATCCAGCAGCTTCATTTATCAGAAGACTTGAGCCCTAGGGAAATACAAGAAAATACTTTCTCACTTCAAGCAGCCTGCTGTGCTGCCCTCACTGAGCTGGTGCTCAATGACACCAATGCCCACCAGGTGGTTCAGGAAAATGGTGTATATACAATAGCAAAATTAATTTTACCAAATAAGCAAAAGAATGCAGCAAAAAGTAATCTATTACAGTGTTATGCTTTCAGAGCCTTGAGATTTCTCTTCAGTATGGAAAGAAACAGACCACTCTTTAAAAGACTTTTCCCCACAGACTTGTTTGAGATCTTCATTGACATAGGGCATTATGTACGTGATATCAGTGCTTATGAAGAATTGGTATCCAAGCTGAATTTATTAGTGGAGGATGAACTGAAGCAAATTGCTGAAAATATTGAAAGCATTAATCAGAACAAAGCTCCTTTGAAATATATAGGCAACTATGCAATTTTGGATCATCTTGGAAGTGGAGCTTTTGGCTGTGTTTACAAGGTTAGAAAGCATAGTGGTCAAAATCTTTTAGCAATGAAAGAGGTCAATTTACATAACCCAGCATTTGGGAAGGATAAGAAAGATCGAGACAGCAGCGTAAGGAATATTGTTTCTGAATTAACAATAATTAAAGAGCAGCTTTATCATCCCAACATTGTACGTTATTACAAAACATTTCTGGAAAATGATAGGTTGTACATAGTTATGGAGCTGATAGAAGGAGCCCCGCTTGGAGAGCATTTCAGTTCTTTGAAGGAAAAACATCACCATTTTACTGAAGAAAGACTATGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGATACTTACACAAGGAGAAGAGGATTGTCCATAGAGATCTGACACCAAACAACATTATGTTGGGGGATAAGGACAAAGTAACCGTTACTGACTTTGGCCTGGCAAAGCAAAAACAAGAAAACAGTAAACTCACGTCTGTGGTTGGAACAATCCTGTATTCTTGCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATGTCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCTGTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTAACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGATGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCTAGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCATGAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCAGCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGACGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACATATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAGAATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGACTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCACCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCCTTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGGGCCACAGATGGGCACATTCTTGTGGCAAGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGTGCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTTCCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGCAGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCCCAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGACCCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTGAAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGACCAAGAATCACCCAACCAAAAGATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAI
NFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCY
QEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISE
LNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFV
SDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFS
MERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMK
EVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKE
KRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEP
VPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSS
GAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTG
GTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQL
PPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEES
GYYNFTSNRYHSYPWGTKNHPTKR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58980
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No