cDNA Clone GFP (plant opt)

• Plasmid: pBABED P 3FLAG GFPp 1-9
• Parent Plasmid: pBABED P 3FLAG
• Expressed: 3FLAG-GFPp 1-9
• Tag: FLAG

Sequence of Insert

ggatccgccatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctgattgaactggatggcgatgtgaacggccataaattttttgtgcgcggcgaaggcgaaggcgatgcgaccattggcaaactgagcctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccatttattttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataaagtgtatattaccgcggataaacagaacaacggcattaaagcgaactttaccattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccgtaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSAMRKGEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKLSLKFICTTGKLPVPWPTLVTTLTYGVQC
FSRYPDHMKRHDFFKSAMPEGYVQERTIYFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHKVYITADKQNNGIKANFTIRHN
VEDGSVQLADHYQQNTPIGDGPVLLP*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBABED P 3FLAG GFPp 1-925 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60479
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plant optimised GFP fragment for use in the construction of tripartite reporter and degron systems.
• Low Temp: No