Plasmid
pEX StrepII 3FLAG Bos taurus CDAN1 2A2A 6His C150rf41
Parent Plasmid
pEX C-GST
Expressed
StrepII 3FLAG Bos Taurus CDAN1 2A2A 6His C150rf41
Genbank
Codanin-1 (XP_015320541.1) C15ORF41 (XP_005211557.1)
Tag
6His
3FLAG
StrepII
Species
Bovine
Sequence of Insert
View Sequence
GGATCCGCCACCATGTGGAGCCACCCGCAGTTCGAAAAAATGGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGATTACAAGGATGACGATGACAAGATGGCTGCTGTGCTGGAATCTCTGCTGCGCGAGGAACTGTCTGTGGCTGCTGCTGTTCGTTGGATCGCTCGTTCCGCTCAGTCCTCTGAAGATGACCCTCGTGAAGCTGCTGCTCTGTCCTCTTTGCGTCCCCTGCGTAAAGAGTTCGTCCCCTTCCTGCTGAACTTCCTGCGCGAGCAATCCTCCAGAGTGCTGCCTCAAGGTCCTCCTACTCCTGCTAAGGCTCCTGGTTCCTCTACCGGACTGCCTGGTAGATCTGGTGGTCCTCCTCGTGGTGGTCGTGGTGCTCGTAACCAGCTGTTCCCTCCAATCGAGGCTTCCTCCGCTACCACTGCTGACGCTCCATCTGCTAGACGTGGTGGAAGAAGGCGTGGTCCTGGTCCAGCTAGAGAACGTGGAAGAGGTCCCGGTGGTCCTGAAGAGGGTGTCAGCGGAGAATGCTTGCCTTGGGCTGGTGGACGTCGTCCTCGTTCCTCTGGTTCTCCTAGCTCTCCCTCTCTGGCTCGTTCTGACCCTCCTAACCTGTCCAACCTGGAAGAGTTCCCTCCTGTGGGTTCCGTGCCTTTGGGTTCCGCTGGTACTAAGCCCTCCAGGCGTATCAACCCCACTCCTGTGTCCGAGGAACGCTCCCTGTCTAAGCCCAAGACCTGCTTTACCTCTCCTCCAATCTCCTGCGTGCCCTCCTCTCAACCTTCCGTGCTGGACACTTCTCCCTGGGGTCACGGATTGCCTTCCGGTTGCCGTTCTCTGCAAGAGGAACGCGAGATGCTGCGCAAAGAGCGTTCCAAGCAGCTGCAGCAGTCCCCTACTCCAGCTTGCCCTACCTTGGAGTCCGGTTCTCCCCATCCTTCTCGTACCGGTCACCTGACTGCTGAGCCTGCTGATCCAGCTCGTGTGTCCTCTCACCACCGTCTGGAACTGGTGGCTCTGGTGTACTCCTCCTGCATCTCCGAGAACCTGGTGCCAAACCTGTTCCTCGAGCTGTTCTTCGTGCTCCAGCTGCTGACCGCTCGTCGTATGGTTGCTGCTAAGGACGGCGACCTCGAGACTACTCCTGGTGCTGTGGACTCCCTGGAATCCCCTCTGTTCCAGTCTGTCCACGACTGCGTGTTCTTCGCTGTGAAGGTGCTCGAGCGTCAGTTCCACATCCTGTCTCACCTGGACAAGGGCACCCTCAAGCTGCTGGCTGAGAACGAACGTCTGCTCTGCTTCTCACCCGCTCTGCAAGGTTGTCTGCGTGCTGCTTACGAGGGTTCCGTCGCTAAGGTGTCCCTGGCTATGCCACCTTCTGCTCAGGCTGTGTCCTTCCAGCCTGAGACTGACAACCGTGCTAACTTCTCCTCCGACCGTGCTTTCCACACCTTCAAGAAACAGCGTGACGTGTTCTACGAGGTGCTCCGCGAGTGGGAAGATCGTCACGAGGAACCTGGATGGGACTTCGAGAAAGGCCTGGGTTCCCGTATCCGTGCTATGATGGGTCAACTGTCCGCTGCTTGCTCCCACTCTCACTTCGTGCGCCTGTTCCAAAAGCAGCTCCTGCAGATGTGCCAGTCTCCTGGTGGTGCTGGTGGTACTGTGCTGGGAGAAGCTCCTGACGTGCTGAACATGCTGGGTGCTGACAAGCTGGGACGTCTGCGTCGTCTGCAAGAACGTTTGGTGGCTCCACAGTCCTCCGGTGGACCTTGTCCTCCTCCAACTTTCCCTGGTTGCCAGGGCTTCTTCCGTGACTTTATCCTGTCCGCTTCCAGCTTCCAGTTCAACCAGCACCTGATGGACTCACTGTCCCTGAAGATCCGCGAGCTGAACTCACTGGCTCTGCCTCAGCCTGAACCTTCTGACGAGGATGGCGAGTCTGACGTGGACTGGCAAGGCGAACGTCGTCAGTTCGCTGTGGTCCTGCTGTCTCTGCGTCTGCTGGCTAAGTTCCTGGGTTTCGTGGCTTTCCTGCCTTACCGCGGTCCTGAGCCTCCTCTGACTCGTGAACTGCAGGACTCCATCTTGAGCCTGCGTTCCCAAGTGCCTCCTGTCTTGGATGTGCGTGCTCTGCTGCAACAGGGACTGCGTGCTCGTCGTGCTGTGTTGACTGTCCCTTGGCTGGTCGAGTTCCTGTCTCTGGCTGACCACATCGTGCCCATGCTGGACTACTACCGTTCCGTGTTCACCCTGCTGCTGCACCTCCACCGTTCACTGATCCTGTCCAAAGAATCCGAGGGCGAGATGTGCTTCCTGAACAAGCTGTTGCTGCTGGCCGTGCTCGGCTGGCTGTTCCAGATTCCTACTGTGCCCGAGGACCTGTTCTTCTTGGAAGAGGGCCAGCTGGACGTGCTGGAAGTCGATACTGTGGCTTCTGAGCACGGACTGGACTCCATGCCTGTGGTGGACCAGCAACTGCTGTACACTTGCTGCCCCTACATCGGCGAGCTGCGCAAGTTGTTGGCTTCCTGGGTGTCCGGTTCCTCCGGTCGTTCTGGTGGTTTCGTGCGCAAGATCACCCCTACCACCACCACTGGACTGGGTGCTCAGCCTCCTCAGACCACTCAAGGACTGCAAGCTCAGCTGGCTCAGGCTTTCTTCCACAACCATCCACCTTCTCTGCGCCGTACCGTCGAGTTCGTGGCTGAGCGTATCGGTTCCAACTGCGTCAAGCACATCAAGGCTACCCTGGTGGCTGAACTCGTCAGGCAGGCTGAATCCCTGCTGCAAGAGCAGCTGGTCACCCAGGGTCAAGAAGGCGGAGATCCTGCTCAACTGCTCGAGATCCTGTGCTCCCAACTGTGCCCTCACGGTGCTCAAGCTCTGACTCAGGGTCGTGAATTCTGCCAGAAAAAGTCCCCTGGTGCCGTCCGTGCTTTGCTGCCTGAAGAAACTCCCGCTGCCGTGCTGTCCTCCGCTGAGAACATTGCTGTGTCCCTCGCTACCGAGAAGGCTTGCGCATGGCTGTCCGCTAACATCACCGCTCTGATCCGTCGTGAAGTGAAGGCTGCCGTGTCTCGTACTCTGAGAGCCCAGGGTCCTGAACCTGCTGCTAGAGGCGAGCGTCGTGGTTGTTCTCGTGCTTGCGAACACCACGCTCCTCTGCCTTCACACCTGATCAGCGAGATCAAGGACGTGCTCTCCCTGGCTGTGGGTCCACGTGATCCTGAGGAAGGTGTCGCTCCCGAGTACCTCGAACAACTGTTGGGTCACCTGGGCCAGATGCTGAGGTGCCGTCAATTCTTGTGCCCTCCAGCTGAACAGCACCTCGCTAAGTGCTCCGTGGAACTGGCTTCTCTGCTGGTGGCCGATCAGATCCCTATCCTGGGTCCTCCTGCTCAGCACCAGCTGGAAAGGGGTCAAGCTCGTCGTTTGCTGCTGATGCTGCTGTCCCTGTGGAAGGACGACTTCCAAGTGCCAGTGCCTCTGCAACTGCTCCTGCGTCCTCGTAATGTGGCTCTGCTCGCTGACACTCGTCCCAAAGAATGGGACCTGCTCCTGTTCCTGCTGCGTGAACTGGTCGAGAAGGACCTGATGAAGCGCATGGAAATCAAGGCTTGCCTGGACTCTCTGTACGAGGCTCAGTGGCCTGGTGTTCGTTCACTGCACGTGTTGTCCGGCGAGAACAGATGCCACGTGCCATTCATCCTGAAAGAGGGACAAGTCATCGGTCCCGGTATCCTGCGCATCTTCCAGTCCAGCGTGAACATCGTGCGTTGCCTGGAAGCTCACCCCGAGATGAAGTGGCTGCTCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAGAAGGGAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAATGCACCATCACCATCACCATATGATACTGACCAAAGCCCAGTACGACGAGATAGCCCAGTGCCTAGTGTCTGTGCCGCCTACCAGGCAGAGCCTGAGGAAGCTGAAGCAGAGGTTCCCCAGTCAATCGCAGGCCACTCTGCTGAGCATCTTCTCCCAGGAGTACCAGAAACATATTAAGAGAACACATGCCAAACATCATACTTCAGAAGCGATTGAAAGTTATTACCAGAGGTATCTGAATGGAGTGAGGAAAAATGGAGCTGCCCCAGTGCTCCTGGAGCTAGCCAATGAGGTGGACTATGCACCCTCATTAATGGCTCGGATTATCCTGGAAAGGTTTCTACAGAAGCATGAGGAAGCTCCACCCTCCAAGTCTGTTATAAATAGTATGCTGCGGGACCCTTCTCAGATTCCAGATGGAGTTCTAGCAAATCAGGTCTATCAGTGCATCGTGAATGACTGCTGTTATGGACCACTGGTGGACTGCATCAAACATGCCATTGGTCATGAGCATGAAGTCCTGCTGAGGGACTTGCTTCTGGAGAAAAACCTATCCTTCCTAGATGAAGATCAGCTTCGTGCAAGAGGTTATGACAAAACGCCAGACTTTATTTTACAAGTACCAGTTGCTGTAGAAGGGCACATAATTCACTGGATTGAAAGCAAAGCCTCATTTGGTGATGAATGTAGCCACCATGCCTACCTGCATGACCAGTTCTGGAGCTACTGGAATAGGTTTGGGCCAGGCTTAGTCATCTACTGGTACGGGTTCATCCAGGAGCTGGACTGTAACCGGGAGAGGGGCATCCTGCTCCATGCCTGCTTCCCCACTGACATCGTCACCTTGTGCCACAGTGTTGCTTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MWSHPQFEKMDYKDHDGDYKDHDIDYKDDDDKMAAVLESLLREELSVAAAVRWIARSAQSSEDDPREAAALSSLRPLRKEFVPFLLNFLREQSSRVLPQG
PPTPAKAPGSSTGLPGRSGGPPRGGRGARNQLFPPIEASSATTADAPSARRGGRRRGPGPARERGRGPGGPEEGVSGECLPWAGGRRPRSSGSPSSPSLA
RSDPPNLSNLEEFPPVGSVPLGSAGTKPSRRINPTPVSEERSLSKPKTCFTSPPISCVPSSQPSVLDTSPWGHGLPSGCRSLQEEREMLRKERSKQLQQS
PTPACPTLESGSPHPSRTGHLTAEPADPARVSSHHRLELVALVYSSCISENLVPNLFLELFFVLQLLTARRMVAAKDGDLETTPGAVDSLESPLFQSVHD
CVFFAVKVLERQFHILSHLDKGTLKLLAENERLLCFSPALQGCLRAAYEGSVAKVSLAMPPSAQAVSFQPETDNRANFSSDRAFHTFKKQRDVFYEVLRE
WEDRHEEPGWDFEKGLGSRIRAMMGQLSAACSHSHFVRLFQKQLLQMCQSPGGAGGTVLGEAPDVLNMLGADKLGRLRRLQERLVAPQSSGGPCPPPTFP
GCQGFFRDFILSASSFQFNQHLMDSLSLKIRELNSLALPQPEPSDEDGESDVDWQGERRQFAVVLLSLRLLAKFLGFVAFLPYRGPEPPLTRELQDSILS
LRSQVPPVLDVRALLQQGLRARRAVLTVPWLVEFLSLADHIVPMLDYYRSVFTLLLHLHRSLILSKESEGEMCFLNKLLLLAVLGWLFQIPTVPEDLFFL
EEGQLDVLEVDTVASEHGLDSMPVVDQQLLYTCCPYIGELRKLLASWVSGSSGRSGGFVRKITPTTTTGLGAQPPQTTQGLQAQLAQAFFHNHPPSLRRT
VEFVAERIGSNCVKHIKATLVAELVRQAESLLQEQLVTQGQEGGDPAQLLEILCSQLCPHGAQALTQGREFCQKKSPGAVRALLPEETPAAVLSSAENIA
VSLATEKACAWLSANITALIRREVKAAVSRTLRAQGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAVGPRDPEEGVAPEYLEQLLGHLGQMLR
CRQFLCPPAEQHLAKCSVELASLLVADQIPILGPPAQHQLERGQARRLLLMLLSLWKDDFQVPVPLQLLLRPRNVALLADTRPKEWDLLLFLLRELVEKD
LMKRMEIKACLDSLYEAQWPGVRSLHVLSGENRCHVPFILKEGQVIGPGILRIFQSSVNIVRCLEAHPEMKWLLEGRGSLLTCGDVEENPGPEGRGSLLT
CGDVEENPGPMHHHHHHMILTKAQYDEIAQCLVSVPPTRQSLRKLKQRFPSQSQATLLSIFSQEYQKHIKRTHAKHHTSEAIESYYQRYLNGVRKNGAAP
VLLELANEVDYAPSLMARIILERFLQKHEEAPPSKSVINSMLRDPSQIPDGVLANQVYQCIVNDCCYGPLVDCIKHAIGHEHEVLLRDLLLEKNLSFLDE
DQLRARGYDKTPDFILQVPVAVEGHIIHWIESKASFGDECSHHAYLHDQFWSYWNRFGPGLVIYWYGFIQELDCNRERGILLHACFPTDIVTLCHSVA*
(self cleavage at 2A2A sequence)
PPTPAKAPGSSTGLPGRSGGPPRGGRGARNQLFPPIEASSATTADAPSARRGGRRRGPGPARERGRGPGGPEEGVSGECLPWAGGRRPRSSGSPSSPSLA
RSDPPNLSNLEEFPPVGSVPLGSAGTKPSRRINPTPVSEERSLSKPKTCFTSPPISCVPSSQPSVLDTSPWGHGLPSGCRSLQEEREMLRKERSKQLQQS
PTPACPTLESGSPHPSRTGHLTAEPADPARVSSHHRLELVALVYSSCISENLVPNLFLELFFVLQLLTARRMVAAKDGDLETTPGAVDSLESPLFQSVHD
CVFFAVKVLERQFHILSHLDKGTLKLLAENERLLCFSPALQGCLRAAYEGSVAKVSLAMPPSAQAVSFQPETDNRANFSSDRAFHTFKKQRDVFYEVLRE
WEDRHEEPGWDFEKGLGSRIRAMMGQLSAACSHSHFVRLFQKQLLQMCQSPGGAGGTVLGEAPDVLNMLGADKLGRLRRLQERLVAPQSSGGPCPPPTFP
GCQGFFRDFILSASSFQFNQHLMDSLSLKIRELNSLALPQPEPSDEDGESDVDWQGERRQFAVVLLSLRLLAKFLGFVAFLPYRGPEPPLTRELQDSILS
LRSQVPPVLDVRALLQQGLRARRAVLTVPWLVEFLSLADHIVPMLDYYRSVFTLLLHLHRSLILSKESEGEMCFLNKLLLLAVLGWLFQIPTVPEDLFFL
EEGQLDVLEVDTVASEHGLDSMPVVDQQLLYTCCPYIGELRKLLASWVSGSSGRSGGFVRKITPTTTTGLGAQPPQTTQGLQAQLAQAFFHNHPPSLRRT
VEFVAERIGSNCVKHIKATLVAELVRQAESLLQEQLVTQGQEGGDPAQLLEILCSQLCPHGAQALTQGREFCQKKSPGAVRALLPEETPAAVLSSAENIA
VSLATEKACAWLSANITALIRREVKAAVSRTLRAQGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAVGPRDPEEGVAPEYLEQLLGHLGQMLR
CRQFLCPPAEQHLAKCSVELASLLVADQIPILGPPAQHQLERGQARRLLLMLLSLWKDDFQVPVPLQLLLRPRNVALLADTRPKEWDLLLFLLRELVEKD
LMKRMEIKACLDSLYEAQWPGVRSLHVLSGENRCHVPFILKEGQVIGPGILRIFQSSVNIVRCLEAHPEMKWLLEGRGSLLTCGDVEENPGPEGRGSLLT
CGDVEENPGPMHHHHHHMILTKAQYDEIAQCLVSVPPTRQSLRKLKQRFPSQSQATLLSIFSQEYQKHIKRTHAKHHTSEAIESYYQRYLNGVRKNGAAP
VLLELANEVDYAPSLMARIILERFLQKHEEAPPSKSVINSMLRDPSQIPDGVLANQVYQCIVNDCCYGPLVDCIKHAIGHEHEVLLRDLLLEKNLSFLDE
DQLRARGYDKTPDFILQVPVAVEGHIIHWIESKASFGDECSHHAYLHDQFWSYWNRFGPGLVIYWYGFIQELDCNRERGILLHACFPTDIVTLCHSVA*
(self cleavage at 2A2A sequence)
Vector Type
Bacterial
Antibiotic
Amp
Full Snapgene Map
pEX StrepII 3FLAG Bos taurus CDAN1 2A2A 6His C150rf41155.11 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU63085
Unit Source
Location
PPU
MTA
Low Temp
No
Price
£125.00
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