cDNA Clone C15ORF41/Codanin-1 (Alligator)

• Plasmid: pEX StrepII 3FLAG Alligator CDAN1 2A2A 6His C150rf41
• Parent Plasmid: pEX C-GST
• Expressed: StrepII 3FLAG Alligator CDAN1 2A2A 6His C150rf41
• Genbank: CDAN1 codon optimised XM_019496591.1; C15orf41 XM_006272326.3
• Tag: 6His
• Tag: 3FLAG
• Tag: StrepII
• Species: Alligator mississippiensis

Sequence of Insert

ggatccgccaccatgtggagccacccgcagttcgaaaaaATGGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGATTACAAGGATGACGATGACAAGATGGCagctGCGCTGGAGCTAGTGCTGCGAGAGGAGGTGGCGCCCGGCGCCCTGGTGCACTGGCTCAAGCACGACCCCGGGCGCCGCTCGGCCGAGGAGAACCATATCCATGAAAAGCTGGTATCTCTTAGTTTGCCTCAGAAAGACTTTGTGCCTTTCTTCTTGAATTTTTTAAGGGAGCAAACCGGTCAGATCCTGACCAATGGGCCTTCCACCCCTGCAAAAACACCGAATTCCAAGGCCCTTGGAAACTCTGCTGGCCGTGGGAGCCAAAGGAGAGGCTCAGAAAGGAGGGCAGGAAACACAACAGGGTCAAGCAGCAGCCGAATGCAGCTCTTTACCCAAAAAGGCTCTGCTAACTCCTGTTCCCCAGATACCAGCTTTGCCCCTTCTGCTGTGTCAGGTGGCTCTCCTGCATTTACTGGATCAAACCTGTCTAGCCCCAGCAGTGATTTCCCTAGTGTGGTCTCCAGTGTGAGCCCAAGCTTTGGTTGCAGCCCTGTATTTAACCACAGTGAGAGGCGCTCAGCTCAGAAGGCCAACTTGGGGAACTTTTTTGTGGCCATGCCAGAAGTTCAGCCGACAAGGCGAGGCAGAAGAAAGGGCAACAGCTCCACCAGTACCTCTAGCCGGCAGGTGGCTCGGGATCTTGGACGATGTCTTGCTGATGAGGAGGATGGGAAGAGTGATAACACTTCATGGAGTGGAGGGAGGAAGAAGCGCAATGAAGTGTTGGGTGTCTCTGCCAGTTCCCCACCTGATCAGCTGAACCTCAACAACTTGGAGGAGTTCCCACCCATGTGCGCTTCTTCTGGCTTCACAAACAAAAGTAAGCCATCAAGACGAATTAACCCCACTCCAGTGAGTATTGAGCGCCCGCTCTCTAAACCCAAGATGTGCTTCACTTCTACACCAGTCAGCCAATCTCCAGCTGCTCGGTTTCCATCTGGAGCAAGTCATGAAGCCTTTGTGACCATCCAGGAAGGAAATGTTGCTTCCACTGCAGGGAGCAGCCTTCAAGAGGAGAGAGAGATGCTGAAGAAAGAACGATGCAAGTTGCTGCACCAGGCTTCTTCTCCTGCAGGCCTGTCTCTGGAGCCCAGCACTCCTACCAAGCTTAGCTACACTCGAAGTGCAAGCCTCCCTCCTGAGGGCCATCTGATGACCTGTGCATATCTCAGTAAGGTCTCCTGCAAAAAGCAACTAGATCAGCTGGCCTTGCTTTATTCATCTTGTATAGCAGAAAACCTGGTTCCGAACATCTTCCTGGAGCTCTTTTTTGTTCTGCAGCTGCTAACATCCAGGGGAATATCTGCAGAAGATGGAGAGAGTGATCTGGAATTCAATCAAGGATGTAAAGGTGCAGTAGAGAGGCAGCATTTTCATAATGTGCACAACTGTGTCTATTTTGCTGTCCAGGTCTTGGATCGTCAGTATGAACTTATTTCCCACTTAGAGAAGGGGACCTTGAAACTCTTGGCTGAGAACGAGCGGATTGCGTCCTTTTCCCCTCACCTGCACAAGAGGCTGAGGCAGGCTTATGAAAGCAGCACAGCCAAGGTTTCTCTCCTGCTGCCTTCATCTGTACAATCTGTTTCTTTCCAGCCAGAAACTGATAATCGCTCTAACTTTCCCAGTGACAGGGCATTTCATATTTTTAAGAAGCAAAGGGACATCTTCTATGAGTTGCTGCGTGAATGGGAAGATAACCATGAAAAACCAGCTTGGGATTTTGAAAGAGTCCTGGGCAACAAGATCAGGGCTATGATGGCTCACCTTACAGCAACTTGTAACCACAGCCATTTTGCCAGACTCTTTCAAAAACAGATTATCCAGATGTGTAAAGGTCCCGTTGGTGGTGGCACAAGCTGGGGAGACACCCCAGACCAGGACGTCCTTAATATGTTGGGCTGTGATGATCTGAGTCGGCTGAAGAGGCTGCAGGAGAGATTTGTCGCTCCTCAGAGCATCAGAGGGCCTTGCCCACCCCCTTCCTTCCCAGGGTGCCAGCAATTCTTCAGGGACTTCATCCTCAGCGCAGGAAGCTATCAGTTCAATCAACACCTCATGGATAACCTGTGCCTAAAAATACTTGAGCTGGATGGCCTTACTTTGGTAGAGCATGAGCCTAGCGATGGTGAAGCAGACATGGAGGAACAGGATGAAAAGAAACATTTCACCGTTGTCCTGATGAGCCTCCGGCTCCTGGCAAAATTTCTTGGATTTCTTGTTTTCCTTCCGTATCGCACAGTGGAGCCACCAACAAGAGACTTGCACGAGTCTGCAGTGGCATTAAGAAATCAAAGCCTACCTGTACTGGATGTATTGAAACTCCTGCAGCGAGCTATCCAGGGCCGGCGTACTATCCTCACTATACCTTGGATTGTGGAGTTCCTCTCCCTCGTAGATCATATTGCTCCTTTTCTTGACTACTATGGGACAGTATTTTCCCTCTTGCTGCAAGTGTACAGGTCCATGGTACCCTCTGAAGATAAGGAGATGAGTTTCCTAAACAAGCTGTTGATCCTTGCAGTGTTGGGTTGGCTTTTTCAGTTTCTGTTCCAGATCCCCTCTGTCCCAGAGGAGCTGTTCTTTACCAGTGAAGTCAGACAGGATGGATTGATGATGGATACCATGTCATCTGCTCAGGCCTTGGATTCTGCAACTTTGGTGGATCAGCAGTTACTTTATACCTGCTGTCCATATCTTGGTGAGCTGCGGAAGCTGCTGGCCTCCTTTGTGGCAGGCAGTGGAGCAAAGAATGGTGGCTTTATAAGAAAAATAACCCCAACAGCTGCAGAGTCCTTGGCACCCAAACCTTCTGTCACACAGCAGAAACTGCAGGCAGAGCTGGAGCAGGCCTTCTTCCACAATCAGCCCCCATCACTGCGGAGAACAGTGGAATTTGTGGCAGAGAGGGTGGGtTCCAACTGTGTTAAGCATATCAAGGCCACTCTGGTAGCAGAGCTTGTCAAAAGGGCAGAGGTCATGCTGCAGGAAAAAGTGAAGGAGGACAATACTAATCATGACAAACTGCTAGATGAGGTGTGCACTCAGCTTTATGAAGAAGGGGCACAGGCTTTCCTCAAAGGCAGAGAATTTTGCAGGAAGAAAGGTCCTGAAGCTGTGAGAGTTCTTCTGCCAGAAGAGACGTCTGCAGCTGTCCTGAGCAGTGCAGAGGATATTGCAGTGAGGCTGGCTACTGAAAAGGCATGTACCTGGCTCTCCACCAACATCACAGCACTGATCAAAAGGGAAGTGAGGACCACATTCAGTCGAATGCTGAAAATCCAGGGTCCTTCCTTACCTAGTGAGGATACACAAGAGATGAAGAAGGACTGCCCTCCAGGATGCCAGCATCATGCTCCATTTCCCTCTGAAATCATCAATGAGATCAAAGATGTTCTCTGTATTGCTGTGGGACCAAGGGATGGAGATGAAGCGATTCATTATGCCCATCTGGAGTGCTTGTTGGAGAAGCTGAGTCAAACACTTCAATGCAGAAAGTTCATGTGTCCTACCTCGGAACAGCAGCTGGCTAAGTGTACCATAGAGTTGGCTTCTCTTTTAGTTTCAGACCGCATTCCGATCCAAGGTCTTGAGCTTCAGACCCAGAAATACCCTGAAAGACAGCGATCAAAGAATCATCCCATCTGCAACCTCCTCACATCGCTGCTCTCTGTCTGGAAGGAAGATTTTGTGATGCTTGTTCCAGTGCAGCTGATCTTCAGCAAGAAGAACGTTGGCTATGTTGCAGAAGCTAAGCAGCGAGAGTGGGACTTGTTCCTGTTCATGCTTCATGGCCTGGTTGAGTACAATCTGATGGGAAATGCGGAGATAGAGAGCTACTTGCAAAGTCTCCAGGAATTTGCCTGGCCCTCAGACTTCTTAAAAGAGCTGGAAACTCTGTCCAGAGTGTTTGAATCTGAGCTTCATATAGAAGAACTCAGGACTAATCCTTGTGAATTGGTGAGACAATCTCTAGGCGCTGTGACAGCACAAAGCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAGAAGGGAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAatgcaccatcaccatcaccatATGAAGTTGAGCAAGGCGCAGTACGAGGAGATCGCCCGGTTCCTGGGGCACGTGCAGCCCACCCGCCAGAGCCTGAGGCGGCTGAAGGAGCGATTCCCCAGTCAATCGCAGTCCACTCTGCTGAGCATCTTCTCCCAGGAGTACCAGAAACAAATCAAAAGAACACATGCCAAACATCACACTGCTGAGGCTATTGAAACTTACTATCAAAGGTACCGGAGTAGGGTGATGAAAAATGCAGCTGCCCCTGTATTATTGGAGCTAGCCAATGAGGTGGACTTTGCACCATCATTGATGGCTCGAATTGTGCTGGAAAGATTTTTACAAGAACAGGAGCAAGGTATCCCATCCAAGACTCTTATAAACAGTATGTTACGGGACCCTTCTCAGATTCCAGATGGAGTTCTAGCAAATCAGGTCTATCAGTGTACTGTGAATGACTGTTGTTATGGACCACTGGTGGACTGCATCAAACATGCTATTGGACATGAACATGAAGTCTTGCTACGAGAAATGCTTCTGAAGAAAAATCTCTCTTTCCTAGCTGAAGATCAGCTTCGTGCAAAGGGTTATGACAAAACACCAGACTTTATTTTGGAAGTGCCAGTAGCTGTTGAAGGCCACATAATTCACTGGATTGAAAGTAAAGCTTCATTTGGTGATGAAAGCAGCCACCAGTCATACCTTCAGGACCAATTCTGGAGCTACTGGAATAGATTTGGTCCTGGATTAGTCATCTATTGGTATGGTTTTATTGAAGAGCTAGACTGTCATCGGGAACGCGGTATCCTGCTAAAAGACTGTTTTCCTACTGACATTGTAACACTGCGACACAGCATGGCTCAACACTGAgcggccgc

Amino Acid Sequence

MWSHPQFEKMDYKDHDGDYKDHDIDYKDDDDKMAAALELVLREEVAPGALVHWLKHDPGRRSAEENHIHEKLVSLSLPQKDFVPFFLNFLREQTGQILTN
GPSTPAKTPNSKALGNSAGRGSQRRGSERRAGNTTGSSSSRMQLFTQKGSANSCSPDTSFAPSAVSGGSPAFTGSNLSSPSSDFPSVVSSVSPSFGCSPV
FNHSERRSAQKANLGNFFVAMPEVQPTRRGRRKGNSSTSTSSRQVARDLGRCLADEEDGKSDNTSWSGGRKKRNEVLGVSASSPPDQLNLNNLEEFPPMC
ASSGFTNKSKPSRRINPTPVSIERPLSKPKMCFTSTPVSQSPAARFPSGASHEAFVTIQEGNVASTAGSSLQEEREMLKKERCKLLHQASSPAGLSLEPS
TPTKLSYTRSASLPPEGHLMTCAYLSKVSCKKQLDQLALLYSSCIAENLVPNIFLELFFVLQLLTSRGISAEDGESDLEFNQGCKGAVERQHFHNVHNCV
YFAVQVLDRQYELISHLEKGTLKLLAENERIASFSPHLHKRLRQAYESSTAKVSLLLPSSVQSVSFQPETDNRSNFPSDRAFHIFKKQRDIFYELLREWE
DNHEKPAWDFERVLGNKIRAMMAHLTATCNHSHFARLFQKQIIQMCKGPVGGGTSWGDTPDQDVLNMLGCDDLSRLKRLQERFVAPQSIRGPCPPPSFPG
CQQFFRDFILSAGSYQFNQHLMDNLCLKILELDGLTLVEHEPSDGEADMEEQDEKKHFTVVLMSLRLLAKFLGFLVFLPYRTVEPPTRDLHESAVALRNQ
SLPVLDVLKLLQRAIQGRRTILTIPWIVEFLSLVDHIAPFLDYYGTVFSLLLQVYRSMVPSEDKEMSFLNKLLILAVLGWLFQFLFQIPSVPEELFFTSE
VRQDGLMMDTMSSAQALDSATLVDQQLLYTCCPYLGELRKLLASFVAGSGAKNGGFIRKITPTAAESLAPKPSVTQQKLQAELEQAFFHNQPPSLRRTVE
FVAERVGSNCVKHIKATLVAELVKRAEVMLQEKVKEDNTNHDKLLDEVCTQLYEEGAQAFLKGREFCRKKGPEAVRVLLPEETSAAVLSSAEDIAVRLAT
EKACTWLSTNITALIKREVRTTFSRMLKIQGPSLPSEDTQEMKKDCPPGCQHHAPFPSEIINEIKDVLCIAVGPRDGDEAIHYAHLECLLEKLSQTLQCR
KFMCPTSEQQLAKCTIELASLLVSDRIPIQGLELQTQKYPERQRSKNHPICNLLTSLLSVWKEDFVMLVPVQLIFSKKNVGYVAEAKQREWDLFLFMLHG
LVEYNLMGNAEIESYLQSLQEFAWPSDFLKELETLSRVFESELHIEELRTNPCELVRQSLGAVTAQSEGRGSLLTCGDVEENPGPEGRGSLLTCGDVEEN
PGPMHHHHHHMKLSKAQYEEIARFLGHVQPTRQSLRRLKERFPSQSQSTLLSIFSQEYQKQIKRTHAKHHTAEAIETYYQRYRSRVMKNAAAPVLLELAN
EVDFAPSLMARIVLERFLQEQEQGIPSKTLINSMLRDPSQIPDGVLANQVYQCTVNDCCYGPLVDCIKHAIGHEHEVLLREMLLKKNLSFLAEDQLRAKG
YDKTPDFILEVPVAVEGHIIHWIESKASFGDESSHQSYLQDQFWSYWNRFGPGLVIYWYGFIEELDCHRERGILLKDCFPTDIVTLRHSMAQH*

(self cleavage at 2A2A sequence)

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pEX StrepII 3FLAG Alligator CDAN1 2A2A 6His C150rf41 78.21 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU63108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No