cDNA Clone GFP-ATG2A

• Plasmid: pBABE puro GFP ATG2A
• Parent Plasmid: pBABE puro GFP
• Expressed: GFP-ATG2A
• Tag: GFP

Sequence of Insert

GGATCCATGTCACGATGGCTGTGGCCATGGTCAAACTGTGTGAAAGAGCGGGTCTGCCGCTACTTGCTGCACCACTACTTAGGTCACTTCTTCCAAGAGCACCTCAGCCTGGACCAGCTCAGCCTCGATCTGTACAAGGGCAGCGTTGCCCTGCGAGACATCCACCTGGAAATCTGGTCTGTGAACGAGGTGCTGGAGTCAATGGAGTCACCGCTGGAGCTGGTGGAAGGCTTCGTGGGCTCCATCGAGGTGGCCGTGCCCTGGGCTGCTCTGCTCACCGACCACTGCACAGTGCGCGTGTCCGGCCTCCAGCTCACCTTGCAGCCCCGCCGGGGTCCAGCGCCAGGGGCTGCCGACTCACAGAGCTGGGCCTCATGCATGACCACAAGCCTGCAGCTGGCCCAGGAGTGTCTGCGGGATGGGCTACCGGAGCCCTCTGAGCCACCACAGCCCCTGGAGGGGCTGGAGATGTTTGCCCAGACCATTGAGACTGTGCTTCGGAGGATCAAAGTGACCTTCCTGGACACTGTCGTGAGGGTGGAGCACTCTCCGGGTGATGGGGAACGTGGTGTGGCCGTCGAGGTCCGTGTGCAGAGACTGGAGTACTGTGATGAGGCAGTGCGGGACCCAAGCCAGGCGCCGCCGGTGGATGTGCATCAGCCGCCTGCCTTCCTGCACAAGCTGCTGCAGCTGGCAGGGGTCCGCCTGCACTACGAGGAGCTCCCGGCACAGGAAGAGCCTCCAGAGCCCCCCTTGCAGATCGGCAGCTGCTCAGGGTACATGGAGCTGATGGTGAAGTTGAAGCAAAATGAGGCCTTCCCTGGCCCCAAGTTGGAGGTGGCGGGACAGCTGGGCTCCCTGCACCTGCTCCTGACCCCGAGGCAGCTCCAGCAACTTCAGGAACTGCTCAGCGCCGTGAGCCTTACAGACCACGAGGGCCTGGCTGACAAGCTGAACAAGAGCCGCCCGCTAGGTGCCGAAGACCTGTGGCTGATTGAGCAGGACCTGAACCAGCAGCTGCAGGCAGGGGCAGTGGCTGAGCCCCTCAGCCCAGACCCCCTTACCAACCCCCTTCTCAACCTGGATAACACTGACCTCTTCTTCTCCATGGCTGGCCTCACAAGCAGTGTGGCCTCAGCCCTCTCTGAGCTCTCCCTCTCCGATGTAGACCTGGCCTCCTCTGTGCGCAGCGACATGGCCTCCCGCCGGCTCTCTGCCCAGGCCCACCCAGCTGGCAAGATGGCCCCCAACCCCCTCCTGGACACCATGCGCCCTGACTCGCTGCTGAAGATGACCTTGGGGGGTGTGACCCTGACCTTGCTTCAGACGTCTGCCCCATCTTCCGGACCACCTGACCTCGCCACGCACTTTTTCACCGAGTTTGATGCCACCAAGGATGGGCCCTTCGGTTCCCGAGACTTCCATCACCTTCGACCACGCTTCCAGAGGGCCTGTCCCTGTAGCCATGTTCGGCTAACGGGCACAGCCGTGCAGCTGTCCTGGGAGCTGCGGACGGGCAGTCGGGGCCGGCGGACAACCAGCATGGAAGTGCACTTCGGGCAGCTGGAGGTGCTGGAGTGTCTGTGGCCCCGGGGCACCTCTGAGCCTGAGTACACGGAGATCCTGACCTTTCCTGGTACGCTGGGCTCCCAGGCCTCAGCTCGGCCCTGCGCCCATCTGCGCCACACACAGATCCTGCGCCGTGTGCCTAAGAGCCGACCCCGGCGCTCAGTTGCCTGCCATTGCCACTCAGAACTGGCCCTGGACCTGGCCAACTTCCAGGCGGACGTGGAGCTGGGGGCCCTGGACCGGCTGGCCGCCCTACTGCGCCTGGCCACCGTACCTGCTGAGCCTCCAGCCGGCCTGCTGACAGAGCCCCTGCCGGCGATGGAGCAGCAGACGGTATTTCGGCTCTCTGCACCCCGGGCCACGCTGCGGCTGCGCTTCCCCATTGCCGACCTGCGGCCTGAGCGGGACCCCTGGGCGGGCCAGGCCGTGCGGGCTGAGCAGCTTCGGCTGGAGCTGAGTGAGCCCCAGTTCCGGTCAGAGCTTAGCAGTGGGCCTGGTCCCCCAGTCCCCACCCACCTGGAACTCACCTGCTCCGACCTACATGGTATCTATGAAGATGGAGGGAAGCCACCTGTCCCTTGCCTGCGTGTCTCCAAAGCCCTGGACCCCAAGAGCACTGGGCGCAAGTACTTCCTGCCCCAGGTAGTGGTGACTGTGAACCCCCAGTCCAGCAGCACACAGTGGGAGGTGGCCCCGGAGAAGGGAGAGGAACTGGAGCTGTCAGTGGAGAGTCCCTGTGAGCTGCGGGAACCTGAGCCCTCGCCCTTCTCCTCTAAGAGGACCATGTATGAGACAGAGGAGATGGTGATCCCTGGAGACCCTGAGGAGATGAGGACGTTCCAGAGCCGGACCCTGGCACTGTCCCGCTGCAGCCTGGAAGTGATCCTGCCCAGTGTCCACATCTTTCTGCCCAGCAAGGAGGTCTACGAGAGCATCTACAACAGGATCAACAACGACCTGCTCATGTGGGAGCCTGCAGATCTGCTTCCCACCCCCGACCCCGCCGCCCAGCCCTCGGGCTTCCCCGGCCCCTCAGGCTTCTGGCACGACAGCTTTAAGATGTGCAAGTCAGCCTTCAAGCTGGCCAACTGCTTTGATCTCACCCCAGACTCGGACTCGGATGACGAGGATGCCCACTTCTTCTCAGTGGGGGCATCAGGTGGCCCACAGGCCGCTGCCCCTGAGGCCCCAAGTCTTCACTTGCAGAGCACCTTCTCTACACTGGTGACAGTGCTGAAGGGGCGGATCACAGCCCTCTGTGAGACCAAGGATGAGGGTGGGAAGCGGCTGGAGGCTGTGCACGGGGAGCTGGTGCTGGACATGGAGCACGGTACCCTCTTCAGCGTCTCCCAGTACTGTGGCCAGCCAGGACTTGGCTACTTCTGTCTGGAAGCTGAAAAGGCAACACTCTACCACCGAGCGGCCGTGGATGACTACCCGCTGCCCAGTCACCTGGACCTTCCCAGTTTCGCTCCCCCGGCTCAGCTGGCCCCAACCATCTACCCATCGGAGGAAGGGGTGACCGAGCGGGGAGCCTCGGGCCGCAAGGGCCAGGGCCGGGGACCCCACATGTTGTCCACTGCTGTGCGCATCCACCTGGACCCCCACAAGAATGTGAAGGAGTTCCTGGTGACACTGCGGTTGCACAAAGCCACCTTGCGCCACTACATGGCCCTGCCCGAGCAGAGCTGGCATTCCCAGTTGTTGGAGTTCCTAGACGTGCTGGATGACCCTGTGCTGGGCTACCTGCCCCCGACGGTCATCACCATCCTGCACACACACCTGTTCTCCTGCTCTGTGGACTATAGGCCACTCTACCTCCCAGTGCGTGTCCTCATCACCGCGGAGACCTTCACTCTCTCCAGCAACATCATCATGGACACCTCCACCTTCCTGCTCAGGTTCATCCTCGATGACTCCGCCTTGTACCTGTCCGACAAGTGTGAGGTGGAGACCCTGGACCTGCGGCGAGATTATGTCTGTGTTTTGGATGTTGACCTCTTGGAACTTGTGATTAAAACCTGGAAAGGGAGCACCGAGGGCAAACTGAGCCAGCCACTATTCGAGCTGCGCTGCTCCAACAATGTGGTACACGTGCACAGCTGTGCCGACTCCTGTGCCCTGCTGGTCAACCTGCTCCAGTACGTAATGAGCACAGGCGATCTGCACCCCCCACCCCGGCCCCCCAGCCCCACGGAGATCGCCGGCCAGAAGCTCTCGGAGAGTCCTGCCTCTCTGCCCTCGTGCCCCCCAGTGGAGACGGCCCTCATCAACCAGCGTGACCTGGCCGACGCCCTCCTGGACACCGAGCGCAGCCTACGGGAGCTGGCCCAGCCTTCAGGTGGCCACCTCCCTCAGGCGTCGCCCATCTCCGTCTACCTATTCCCAGGTGAACGGAGTGGGGCCCCACCCCCTTCACCACCTGTCGGGGGCCCTGCTGGCAGCTTAGGGTCATGCTCAGAGGAGAAGGAAGATGAAAGGGAAGAGGAGGGCGATGGAGACACCCTGGACAGTGATGAGTTCTGCATCCTTGATGCTCCCGGCCTGGGCATCCCGCCCCGAGATGGGGAGCCTGTGGTGACACAGCTGCATCCCGGCCCCATCGTTGTGAGGGACGGTTACTTCTCACGGCCGATCGGCAGCACGGACTTGCTGCGGGCACCTGCCCATTTCCCAGTGCCCAGCACTCGGGTGGTGCTACGTGAGGTCTCCCTCGTCTGGCACCTCTATGGGGGCCGAGACTTTGGCCCCCACCCCGGCCACAGGGCAAGAACTGGCCTCTCAGGTCCCAGGAGCTCCCCTTCCCGCTGCTCTGGCCCCAACCGGCCCCAGAACTCATGGCGCACGCAGGGGGGCAGCGGGCGGCAGCACCATGTCCTCATGGAGATCCAGCTGAGCAAGGTAAGCTTCCAGCACGAGGTGTACCCAGCGGAGCCAGCCACAGGCCCTGCGGCCCCCAGCCAGGAGCTGGAGGAGCGACCGCTGTCCCGTCAGGTGTTCATCGTGCAGGAGCTGGAGGTCCGAGACCGGCTCGCCTCCTCCCAGATCAACAAGTTCCTGTACCTACACACGAGTGAGCGGATGCCGCGACGTGCCCACTCTAACATGCTCACCATCAAAGCGCTGCATGTGGCCCCCACTACCAACCTGGGTGGGCCTGAGTGCTGTCTCCGCGTCTCGCTGATGCCCCTGCGGCTCAATGTGGACCAGGATGCCCTCTTCTTCCTCAAGGACTTCTTCACTAGTCTGGTGGCCGGCATCAACCCCGTGGTCCCAGGGGAGACCTCCGCTGAGGCTCGCCCCGAGACTCGAGCCCAGCCCAGCAGCCCCCTGGAAGGGCAGGCCGAAGGCGTAGAGACCACTGGTTCGCAGGAGGCCCCAGGAGGTGGACACAGCCCCTCCCCTCCTGACCAGCAGCCCATCTACTTCAGAGAGTTCCGCTTCACGTCTGAGGTCCCCATCTGGCTGGATTACCATGGCAAGCACGTCACGATGGACCAGGTGGGCACTTTTGCTGGCCTCCTCATCGGCCTGGCCCAACTCAACTGCTCCGAGCTGAAGCTAAAGCGGCTCTGTTGCAGGCACGGGCTCCTGGGTGTGGACAAGGTGCTGGGCTATGCCCTCAACGAGTGGCTGCAGGACATCCGCAAGAACCAGCTGCCCGGCCTGCTGGGAGGCGTGGGCCCCATGCACTCGGTTGTCCAGCTCTTCCAAGGGTTCCGGGACCTGCTGTGGCTGCCCATTGAGCAGTACAGGAAGGATGGCCGCCTCATGCGGGGGCTGCAGCGAGGGGCTGCCTCCTTTGGCTCATCCACAGCCTCTGCCGCCCTGGAACTCAGCAACCGGTTGGTACAGGCTATCCAGGCCACAGCTGAGACCGTGTATGACATCCTGTCCCCGGCAGCCCCCGTCTCCCGCTCCCTGCAGGATAAGCGCTCTGCGCGGAGGCTGCGCAGGGGCCAGCAGCCTGCCGACCTGCGGGAGGGTGTGGCCAAGGCCTACGACACAGTGCGAGAGGGCATCTTGGATACAGCTCAGACCATCTGTGACGTGGCATCGCGGGGCCATGAGCAGAAGGGGCTGACGGGCGCCGTGGGGGGCGTGATCCGCCAGCTGCCCCCGACTGTGGTGAAGCCGCTCATCCTGGCCACGGAGGCCACGTCCAGCCTGCTCGGGGGCATGCGCAACCAGATTGTCCCCGACGCCCACAAGGACCACGCCCTCAAGTGGCGCTCGGACAGTGCCCAAGACTGAGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSRWLWPWSNCVKERVCRYLLHHYLGHFFQEHLSLDQLSLDLYKGSVALRDIHLEIWSVNEVLESMESPLELVEGFVGSIEVAVPWAALLTDHCTVRVSGLQLTLQPRRGPAPGAADSQSWASCMTTSLQLAQECLRDGLPEPSEPPQPLEGLEMFAQTIETVLRRIKVTFLDTVVRVEHSPGDGERGVAVEVRVQRLEYCDEAVRDPSQAPPVDVHQPPAFLHKLLQLAGVRLHYEELPAQEEPPEPPLQIGSCSGYMELMVKLKQNEAFPGPKLEVAGQLGSLHLLLTPRQLQQLQELLSAVSLTDHEGLADKLNKSRPLGAEDLWLIEQDLNQQLQAGAVAEPLSPDPLTNPLLNLDNTDLFFSMAGLTSSVASALSELSLSDVDLASSVRSDMASRRLSAQAHPAGKMAPNPLLDTMRPDSLLKMTLGGVTLTLLQTSAPSSGPPDLATHFFTEFDATKDGPFGSRDFHHLRPRFQRACPCSHVRLTGTAVQLSWELRTGSRGRRTTSMEVHFGQLEVLECLWPRGTSEPEYTEILTFPGTLGSQASARPCAHLRHTQILRRVPKSRPRRSVACHCHSELALDLANFQADVELGALDRLAALLRLATVPAEPPAGLLTEPLPAMEQQTVFRLSAPRATLRLRFPIADLRPERDPWAGQAVRAEQLRLELSEPQFRSELSSGPGPPVPTHLELTCSDLHGIYEDGGKPPVPCLRVSKALDPKSTGRKYFLPQVVVTVNPQSSSTQWEVAPEKGEELELSVESPCELREPEPSPFSSKRTMYETEEMVIPGDPEEMRTFQSRTLALSRCSLEVILPSVHIFLPSKEVYESIYNRINNDLLMWEPADLLPTPDPAAQPSGFPGPSGFWHDSFKMCKSAFKLANCFDLTPDSDSDDEDAHFFSVGASGGPQAAAPEAPSLHLQSTFSTLVTVLKGRITALCETKDEGGKRLEAVHGELVLDMEHGTLFSVSQYCGQPGLGYFCLEAEKATLYHRAAVDDYPLPSHLDLPSFAPPAQLAPTIYPSEEGVTERGASGRKGQGRGPHMLSTAVRIHLDPHKNVKEFLVTLRLHKATLRHYMALPEQSWHSQLLEFLDVLDDPVLGYLPPTVITILHTHLFSCSVDYRPLYLPVRVLITAETFTLSSNIIMDTSTFLLRFILDDSALYLSDKCEVETLDLRRDYVCVLDVDLLELVIKTWKGSTEGKLSQPLFELRCSNNVVHVHSCADSCALLVNLLQYVMSTGDLHPPPRPPSPTEIAGQKLSESPASLPSCPPVETALINQRDLADALLDTERSLRELAQPSGGHLPQASPISVYLFPGERSGAPPPSPPVGGPAGSLGSCSEEKEDEREEEGDGDTLDSDEFCILDAPGLGIPPRDGEPVVTQLHPGPIVVRDGYFSRPIGSTDLLRAPAHFPVPSTRVVLREVSLVWHLYGGRDFGPHPGHRARTGLSGPRSSPSRCSGPNRPQNSWRTQGGSGRQHHVLMEIQLSKVSFQHEVYPAEPATGPAAPSQELEERPLSRQVFIVQELEVRDRLASSQINKFLYLHTSERMPRRAHSNMLTIKALHVAPTTNLGGPECCLRVSLMPLRLNVDQDALFFLKDFFTSLVAGINPVVPGETSAEARPETRAQPSSPLEGQAEGVETTGSQEAPGGGHSPSPPDQQPIYFREFRFTSEVPIWLDYHGKHVTMDQVGTFAGLLIGLAQLNCSELKLKRLCCRHGLLGVDKVLGYALNEWLQDIRKNQLPGLLGGVGPMHSVVQLFQGFRDLLWLPIEQYRKDGRLMRGLQRGAASFGSSTASAALELSNRLVQAIQATAETVYDILSPAAPVSRSLQDKRSARRLRRGQQPADLREGVAKAYDTVREGILDTAQTICDVASRGHEQKGLTGAVGGVIRQLPPTVVKPLILATEATSSLLGGMRNQIVPDAHKDHALKWRSDSAQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59537
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent C645T, SNP P656R
• Low Temp: No