cDNA Clone NEK10

• Plasmid: pcDNA5D FRT/TO GFP Nek10 tv 2 (long) D673A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP NEK10 tv 2 (long) D673A
• Genbank: NM_152534.4 with 5 silent changes
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCTGATCAAGATAAAAAGGTGAAGACCACAGAAAAATCAACTGATAAACAGCAAGAAATCACCATCAGGGACTATTCAGATCTTAAAAGACTTCGGTGCCTTTTGAACGTCCAATCAAGCAAACAACAGCTTCCAGCCATTAACTTCGATAGTGCCCAAAATAGCATGACGAAGTCTGAGCCCGCCATCAGGGCGGGTGGACACAGAGCTCGGGGTCAGTGGCATGAATCCACAGAAGCTGTTGAACTTGAAAATTTTAGTATAAACTACAAGAATGAGAGAAATTTCAGCAAACATCCTCAGCGTAAACTATTTCAGGAGATCTTTACCGCCTTGGTGAAAAATAGACTCATAAGCAGAGAGTGGGTTAATCGAGCCCCATCTATTCATTTTCTGAGAGTGTTAATCTGTCTGAGGCTACTAATGAGGGACCCATGTTATCAGGAAATACTCCATAGCTTGGGTGGGATTGAAAACCTAGCTCAGTATATGGAGATTGTAGCCAATGAGTACCTCGGCTATGGAGAAGAGCAGCACACTGTGGACAAGCTGGTCAACATGACATATATTTTTCAAAAACTTGCTGCAGTCAAAGATCAAAGAGAATGGGTCACCACAAGTGGAGCCCACAAGACATTAGTAAATTTACTTGGTGCCCGAGATACTAATGTTCTATTGGGTTCCCTTCTGGCTCTGGCTAGTTTAGCAGAAAGTCAAGAATGTAGGGAGAAGATAAGTGAACTCAACATTGTAGAAAATCTGTTGATGATTTTACATGAATATGACTTGCTTTCTAAAAGACTAACAGCGGAGTTGCTGCGCCTACTTTGTGCAGAGCCCCAGGTGAAAGAGCAGGTGAAGCTCTATGAGGGGATACCGGTCCTCCTCAGTCTGCTCCACTCTGACCACTTGAAGCTCCTCTGGAGCATTGTCTGGATTCTGGTACAGGTTTGTGAGGACCCTGAGACCAGCGTGGAAATTCGCATTTGGGGAGGCATCAAACAGCTTCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGATCACTCCTCCATTGGAAGCCTGTCCAGTGCAAATGCTGCAGGCCGAATCCAGCAGCTTCATTTATCAGAAGACTTGAGCCCTAGGGAAATACAAGAAAATACTTTCTCACTTCAAGCAGCCTGCTGTGCTGCCCTCACTGAGCTGGTGCTCAATGACACCAATGCCCACCAGGTGGTTCAGGAAAATGGTGTATATACAATAGCAAAATTAATTTTACCAAATAAGCAAAAGAATGCAGCAAAAAGTAATCTATTACAGTGTTATGCTTTCAGAGCCTTGAGATTTCTCTTCAGTATGGAAAGAAACAGACCACTCTTTAAAAGACTTTTCCCCACAGACTTGTTTGAGATCTTCATTGACATAGGGCATTATGTACGTGATATCAGTGCTTATGAAGAATTGGTATCCAAGCTGAATTTATTAGTGGAGGATGAACTGAAGCAAATTGCTGAAAATATTGAAAGCATTAATCAGAACAAAGCTCCTTTGAAATATATAGGCAACTATGCAATTTTGGATCATCTTGGAAGTGGAGCTTTTGGCTGTGTTTACAAGGTTAGAAAGCATAGTGGTCAAAATCTTTTAGCAATGAAAGAGGTCAATTTACATAACCCAGCATTTGGGAAGGATAAGAAAGATCGAGACAGCAGCGTAAGGAATATTGTTTCTGAATTAACAATAATTAAAGAGCAGCTTTATCATCCCAACATTGTACGTTATTACAAAACATTTCTGGAAAATGATAGGTTGTACATAGTTATGGAGCTGATAGAAGGAGCCCCGCTTGGAGAGCATTTCAGTTCTTTGAAGGAAAAACATCACCATTTTACTGAAGAAAGACTATGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGATACTTACACAAGGAGAAGAGGATTGTCCATAGAGATCTGACACCAAACAACATTATGTTGGGGGATAAGGACAAAGTAACCGTTACTGCCTTTGGCCTGGCAAAGCAAAAACAAGAAAACAGTAAACTCACGTCTGTGGTTGGAACAATCCTGTATTCTTGCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATGTCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCTGTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTAACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGATGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCTAGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCATGAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCAGCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGACGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACATATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAGAATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGACTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCACCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCCTTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGGGCCACAGATGGGCACATTCTTGTGGCAAGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGTGCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTTCCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGCAGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCCCAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGACCCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTGAAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGACCAAGAATCACCCAACCAAAAGATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNS
MTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLG
GIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLL
MILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGS
LSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLF
KRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPA
FGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDL
TPNNIMLGDKDKVTVTAFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSE
KVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSE
LSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRP
ALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNL
KRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSN
RYHSYPWGTKNHPTKR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63147
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No