cDNA Clone NDFIP2

• Plasmid: pBabe puro NDFIP2-GFP
• Parent Plasmid: pBabe puro C-GFP
• Expressed: NDFIP2-GFP
• Genbank: NC_000013.11
• Tag: GFP

Sequence of Insert

GGATCTATGGCACGCCGGCGGAGCCAGCGAGTCTGCGCGAGCGGTCCGAGCATGCTCAATAGCGCGCGCGGCGCCCCGGAGCTTCTCCGCGGAACCGCGACCAACGCGGAGGTCTCGGCGGCCGCTGCGGGAGCCACAGGAAGTGAAGAGCTTCCGCCGGGAGACCGCGGCTGCAGGAACGGAGGCGGAAGGGGCCCTGCGGCGACGACGTCGTCGACGGGGGTGGCCGTGGGAGCTGAGCACGGAGAAGACTCCCTCTCTCGGAAGCCGGATCCCGAGCCGGGCAGGATGGATCACCACCAGCCGGGGACTGGGCGCTACCAGGTGCTTCTTAATGAAGAGGATAACTCAGAATCATCGGCTATAGAGCAGCCACCTACTTCAAACCCAGCACCGCAGATTGTGCAGGCTGCGTCTTCAGCACCAGCACTTGAAACTGACTCTTCCCCTCCACCATATAGTAGTATTACTGTGGAAGTACCTACAACTTCAGATACAGAAGTTTACGGTGAGTTTTATCCCGTGCCACCTCCCTATAGCGTTGCTACCTCTCTTCCTACATACGATGAAGCTGAGAAGGCTAAAGCTGCTGCAATGGCAGCTGCAGCAGCAGAAACATCTCAAAGAATTCAGGAGGAAGAGTGTCCACCAAGAGATGACTTCAGTGATGCAGACCAGCTCAGAGTGGGGAATGATGGCATTTTCATGCTGGCATTTTTCATGGCATTTATTTTCAACTGGCTTGGATTTTGTTTATCCTTCTGTATCACCAATACCATAGCTGGAAGGTATGGTGCTATCTGCGGATTTGGCCTTTCCTTGATCAAATGGATCCTTATTGTCAGGTTTTCTGATTATTTTACTGGATATTTCAATGGACAGTATTGGCTTTGGTGGATATTTCTTGTACTTGGCCTGCTCCTTTTCTTCAGAGGATTTGTTAATTATCTAAAAGTCAGAAACATGTCTGAAAGTATGGCAGCTGCTCATAGAACAAGGTATTTCTTCTTATTGGCGGCCGC

Amino Acid Sequence

MARRRSQRVCASGPSMLNSARGAPELLRGTATNAEVSAAAAGATGSEELPPGDRGCRNGGGRGPAATTSSTGVAVGAEHGEDSLSRKPDPEPGRMDHHQPGTGRYQVLLNEEDNSESSAIEQPPTSNPAPQIVQAASSAPALETDSSPPPYSSITVEVPTTSDTEVYGEFYPVPPPYSVATSLPTYDEAEKAKAAAMAAAAAETSQRIQEEECPPRDDFSDADQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLLAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59541
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: after RTPCR found insertion of 61 nt after nt 715 compared to requested NM_001161407.1, results in this being variant 1 NM_019080.2 (Has internal Not site)
• Low Temp: No