cDNA Clone Gip3 (ypl137c)

• Plasmid: pRS413-ADH Pr yeast -800-Gip3+300
• Parent Plasmid: pRS413-ADH Pr
• Expressed: yeast GIP3 including 800 bp promoter and 300 bp 3'UTR

Sequence of Insert

GAATTCAAGAGCGAAGGAAGAGAGCAGAAAAGGCTACAACGAAACCCTTTCATTTACTTATATAAATAGCCTGAGTCATTTTTCCTCTCAATTTTTGCATCATCATGATCATACATGCTTTTTTTTTCCTTCTCCATGGCTAATTGGGGTGCAACGCACCTTATCGCCTTTGACGGCCTTGGCTAAGCGGAGATAAAGCCTCCGAACTTCGGCCCCGAAGGGATTGGCGCGGATATACACGGAAATAGCTTGTCATTTTTCCTTCGGTTGATGTCCGCGGACCGAGAAGTGGTGTTTCCACATTACGTCTCAGTGATGCAATCATCATGGTACGGGCATTAATTAAGGTTAAGGAACCACTTCTCTACGCCATACTCCAAACAATAACAATATTAGACCTAGCCAATTGATACCCATGAGCGTGCAGTCATTGCCATGGATTATTCATCTCTTTTCGCTGTTCTCTTTCTTTTCAAGTTTGTCATCATCATGCCTTCACTTTTGCCTTTCCATCTTTCTTTTTGCTGCAAAATAAAGGGAAGAGGGGTAAAAACGCAAGGAAGAACAAGAAGAAGAGGGTAGTGCAAGAAAAAGAAAAGAAGAAAAAAAAAAAAAAGTAATCTTGATACCGTGAGCAAATAAGCTAACGGAAAGCGTAAGAAAGAAGAGCGTGTTTTGGGAAATAACACCACAGCATAAAGCTAAAATTCAGTTTATATAATCTATAGTAGTCCTATAGAAATTGCGAATAACGGAAACAATAGTCCACCAAAGCAAGCATAGGGAGTGGAGATAGCATCTAGGTTATGATAACTAACACCGAGTTCGATGTGCCCGTAGATTGGTTGTATAAAGGTAAGAGTAGACGGAAGACAAATACGAAGCCATCGAGGCCTTCTACGTCGCCAGCCTCCTCTTCATCTACGTCCTCTTCGAAAAACGGAGACAATAGCACGAGTGGTAACAGGTCTAGCAATGATAAGCCTCGTGCGAGGTCGTCATCTGTATCCAATGCAGCACTTTGTAATACTGAGAAACCAGATTTGAAGAGGAACGATGGTAATACCTCTGCATCAGACACTGATAACATACCGCTACTTACTCCTATTAATAGCGGTAACCGGAGCGATTCCGCTGACATTGATAATCCGGCTACCGTAGACGCCATAGACCTTATAGATAATGATGACAACGGCTCAAGTACTCAATTTGTAAGGAAAAAACGTTCCACTTCTATATCCAATGCTGTGGTCTCGTCCAAACCAAGACTGGCCAGTTCTGCCATAAACGCCACCGCATCTTCTTCTGTTGGCAAGGGAAAGCACCCGCCAATTTCTTCACCATCTAATGCTACTCTCAAGAGAAGCAACTCGACTAGTGGAGAAAAGACTAAAAGGTCGATTTTTGGATCTTTGTTTAGCAAACGGTCTACCTCTTCTTCAGCTTCCACTGCCAAGAAACCATTACCTGTTGTTAATACTAGCACGACTGAGAATGAGTCTGGTGGCATTAAAGCAGTTGCGACTCCTGATCCACGGGTGAAGGAGATATCATCACCAATGAGAGGAGTGGCACCAACAGCTAGCAAGCCACAGACACCTATACTCCCCTCCCCTGCACTTGCAGTAAAAGACCTATCTACAGTTTCATTGAAAAGGGTCTCGTTTGCCGTCGATAAATTCGAATCCGATCCTCCCCAACAACTTCCTTCAAGAACCCCAAAAAAGGGAAACATCCTTATCCCTGATGACATGATAAGCGAGGTTCCTTCTATTTCCGTGGGTATTTCCAGTAGCAACCAATCGGCCAAGTCAACCAATTCCAACATTAAGGGACCGTTGTACACTAAGAAGTCTAAAGAATATATACTCGCCCTGGAAAATCAAAAGTTGGCTTTAAGGGAGGCTGCTAAGCATCAGCAAGAAGCTCATTTTGCTGCTAATAGAATAGCCTTTGAAGTAGCCAATTTTAAGACTGCTTCGGATGCAGGCGGTAAGCTTACTGAAAAGTCATCAGAAGGCACCATTACGAAGCAAAGAGAAGAGGTGTCACCTCCAAATGTTGAAGCTGATCGGGAGCTTGAGAATAATAAACTTGCAGAAAACCTCTCAAAAGCCGGCATTGATAAGCCCATTCATATGCATGAGCACTATTTCAAGGAACCTGATCAAGATAAGTACCAAGACGGTCATTCTATTGAAAATAACGAAGTTACGCTGGATGTTATTTACACAAGATGCTGCCATTTAAGGGAAATTTTACCCATTCCGTCTACCCTAAGACAAGTAAAGGACAAGACAGCTCCCTTGCAAATATTGAAGTTTTTGAACCCTAAGCCTACCTTGATCGATATTCTTTCCTTTTGTGATTTCATCACCATTGCTCCCATTCACACGATTGTTTTCGACAATGTAGCTTTAAACCAGGATATGTTCAGAATAATTATTTCTGCTTTGGTAAACTCCACAGTTTTGGACAAATTGAGTTTAAGAAATGTACGAATCGACCAAGATGGATGGAAATTACTATGTAAGTTCCTTTTGCTAAACAAATCACTGAACAAGCTCGACATTTCCCAAACGAAAATCAAATCTGACCTTGCTGAATCACTATATCGCCATAACATGGATTGGAACTTGTTTACTGACGTCTTGTCTCAGAGATCTCACAAACCTATAGAAGAATTGTTATTCAATGGTATTCAATTTAGCAAAATTCCTTACTCATGCTTTGCACGTTTACTGACATCCTTTGCTACCCAAAAAAACTTTCCAGAATCGGGCATCAGGCTTGGTTTAGCAGGTGCCACTACTTCCAATATCTCTCAGGATTGTCTGAAATTTATCTTCAATTGGATGTCTCAATATAACGTCCAGGGTGTGGATCTAGCGTTTAATGATCTGTCCACTATGATTAAGCCAATGGTTGGTAAACTATCTGCTCTATCATATGATAACTTAAGATATTTCATTTTGAACAGCACTAATATTTCGACTTCTTATGATTTAGCTTTGCTCTTGAAATATCTTTCTAAGTTGCCTAACTTGATTTTTTTGGATTTGAGTAATTTGTCGCAATGCTTTCCTGACATTTTACCCTACATGTACAAGTATTTACCAAGATTTCCGAACCTGAAGAGGATACATTTGGATAGCAATAATTTGACATTAAAGGAACTAGCTGTCGTTTGTAATATTTTGATTAAGTGCAAATCGCTCTCGCATGTGTCAATGACTAATCAAAACGTTGAAAACTTCTATCTAATGAACGGCACAGATTCTCCCGTTCAACAAACTAACACAGACGGCGACTTGGATAGTTCGAGCACATTGGACGTTAAGGGCCAATTTGCTAAAAATAGTTTTTCATCCACACTTTACGCATTTGCTAGAGACTCTCCAAACTTGATTGGTTTAGATTTTGACTATGATTTGATATCAGAAGAGATTCAATCAAGAATAGCACTGTGCTTGATGAGAAATATGAAACGGACCATGGATTCGACTTTCCAGTTAGATGAATTGGATTCGCAAGATGATTTGCTATTCGATGGCTCTTTAGTAACTATGACCGCTGAAAGTGTTTTAGAAAAACTGAACTTGTTAAGCGATAAGAGTACAAAGGTCAAAAAAGACACCACGAAGAGATATTTGCTGAAAAAATATATTGAAAAGTTCCATATCTTGCACCATAATGTTCAGCATACAATAGATACTATGTTCGAAAAAAGGAAATCAGGTGAATTGCCATTGCAAGAAAAAGAAAACCTTGTAAGATTACTCCTTTTGGAACAAAACTTATGCAATATACTAGAATTGTTTTCGCATAATCCGAATCTGAATGATGTCCTTGGATCCAGCAGGGATGATTCGAAGGAAAGTGTTGACTCCAGCGAAGATTCAAAATTACCAGCATTGAAGCACGTTGAATCCGGATACCATGTTCCCGAAGAAAAAATACAACCTGAGAATGATGTAATTACAGCAAGACCACATTTAATGGCCACCGATTCTGGTAAAACTATTGACGTTTTCACAGGCAAACCATTGGTGTTCAAGCATACATCATCTAGTACTTCTGTAGGTTGTAAAAAGCAGGAAGAAGAAGAAGGTGAACTACATAAATGGGGTTTCTTCGTTCAGCAGCAGAGGTCTTTATACCCTGAAAATGAATCAACAAGGCAGACACCTTTTGCATCAGGTGATACGCCTATCAACACAGAAACAGCTGGTAAGTCAACTTCATCTCCTTCAGTTTCAACTTCTAACAATGAAACTGCTACCACTAGTTTATTTAGCCCAGCTAATCCTAAGATTTTACCAAAAATTCCATCCGGTGCTGTCTTAAGATCGGCAATCATGAAGGCTAAAGGTATTGATTCTATCGATGATTTGATTCAGAACGTTAACTCCAACAACATAGAATTGGAGAACATTTATGGTGAATCCATTCAGAATAGCGCTTCGACATTTACACCAGGCGTGGATTCTGATGTATCTGCGCCCAATACCGATAAAGGATCCGTAGAAACATTGCCTGCAGTCTCAACTGACGACCCAAATTGTGAAGTCAAAGTCACTGCTACCTATGACAAACTATTAAATAATTTGTCGATGGAGAGGTCAATCAGACTTTGAAGATACATGAATCCTTCTCTTCACTGATGGATCCATTTTCTTCTTCCTTTTTTTCACTGCATAAAACTTTTTTTATCAACCTTTCATATTTTCCATATTTATTTATATATACCGCCAGTAAATTTGGCGATATTCGATAGACTTAACAGATAAACTTTTTAAGATGAATTAAAGCACAGAGGATTAAGCTTACCTCTTTAGAATCAATTGATATGTTCGGCTACATATTAGACACTGCATATAACCCAAGGCGGGTAAAATATGAACAATTTCAATTGGGCTTTGTAAATGCTTACTTTTCTCGAG

Amino Acid Sequence

MITNTEFDVPVDWLYKGKSRRKTNTKPSRPSTSPASSSSTSSSKNGDNSTSGNRSSNDKPRARSSSVSNAALCNTEKPDLKRNDGNTSASDTDNIPLLTPINSGNRSDSADIDNPATVDAIDLIDNDDNGSSTQFVRKKRSTSISNAVVSSKPRLASSAINATASSSVGKGKHPPISSPSNATLKRSNSTSGEKTKRSIFGSLFSKRSTSSSASTAKKPLPVVNTSTTENESGGIKAVATPDPRVKEISSPMRGVAPTASKPQTPILPSPALAVKDLSTVSLKRVSFAVDKFESDPPQQLPSRTPKKGNILIPDDMISEVPSISVGISSSNQSAKSTNSNIKGPLYTKKSKEYILALENQKLALREAAKHQQEAHFAANRIAFEVANFKTASDAGGKLTEKSSEGTITKQREEVSPPNVEADRELENNKLAENLSKAGIDKPIHMHEHYFKEPDQDKYQDGHSIENNEVTLDVIYTRCCHLREILPIPSTLRQVKDKTAPLQILKFLNPKPTLIDILSFCDFITIAPIHTIVFDNVALNQDMFRIIISALVNSTVLDKLSLRNVRIDQDGWKLLCKFLLLNKSLNKLDISQTKIKSDLAESLYRHNMDWNLFTDVLSQRSHKPIEELLFNGIQFSKIPYSCFARLLTSFATQKNFPESGIRLGLAGATTSNISQDCLKFIFNWMSQYNVQGVDLAFNDLSTMIKPMVGKLSALSYDNLRYFILNSTNISTSYDLALLLKYLSKLPNLIFLDLSNLSQCFPDILPYMYKYLPRFPNLKRIHLDSNNLTLKELAVVCNILIKCKSLSHVSMTNQNVENFYLMNGTDSPVQQTNTDGDLDSSSTLDVKGQFAKNSFSSTLYAFARDSPNLIGLDFDYDLISEEIQSRIALCLMRNMKRTMDSTFQLDELDSQDDLLFDGSLVTMTAESVLEKLNLLSDKSTKVKKDTTKRYLLKKYIEKFHILHHNVQHTIDTMFEKRKSGELPLQEKENLVRLLLLEQNLCNILELFSHNPNLNDVLGSSRDDSKESVDSSEDSKLPALKHVESGYHVPEEKIQPENDVITARPHLMATDSGKTIDVFTGKPLVFKHTSSSTSVGCKKQEEEEGELHKWGFFVQQQRSLYPENESTRQTPFASGDTPINTETAGKSTSSPSVSTSNNETATTSLFSPANPKILPKIPSGAVLRSAIMKAKGIDSIDDLIQNVNSNNIELENIYGESIQNSASTFTPGVDSDVSAPNTDKGSVETLPAVSTDDPNCEVKVTATYDKLLNNLSMERSIRL*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast Gip3 (ypl137c) including 1000 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No