cDNA Clone BUL1 (YMR275C)

• Plasmid: pRS413-ADH Pr -800-BUL1-(+)300
• Parent Plasmid: pRS413-ADH Pr
• Expressed: yeast BUL1 (YMR275C) including 800 bp promoter and 300 bp 3'UTR

Sequence of Insert

agatctCTGCAATTGCGGGTTGGACTGGATCATAAAGTCCAACATCTGAGGGTTACTCAACATCTCGTTCATTTGCGATTGGAAGATGGGGTTTTCCATCATCCTCAACAGCTCGTCTTGGTTATTCGAGTCGTTGTTTAATGCACCACCGTCCGGGCCAAACATGTCTGCAGATGGCATATTCAAATATCCAGCGTATCTGGCACTGGTCAAGTCGGCCAGCGGATTGAAGCCTGCACTTTGACCTGACGACATGTTCGGGGTGGCGCCAGTGCCAGCTGCTGCACCGGTGGCGGTGGCGTTATTTGCGCCAGCGGCACTGGCGGTTTGTGGTTTGGGTTGTGACTTGACCAGATGGACGCTGTGACCGTCTTGAATGTGGTATGACTCCACGGTTTGGTCGTCCTTTAAAATCTTACCCGAGTAGATCAATCTCTGATTTGCCACCGGGATACCGTTCGCTTTGTTTATCGCCTCCTTGAATTGCAACACTGTGCTTTCAGGTGCCACATTTACTTCCCACTTGTCTTGTCCTGACTTGATATGTATATTCAACGACATTATCGTGCTAGAATGATTCGGTGTCTTTGATCCGTCTTATTTGCCTCTCGTCCATCGGTGGATTGAACTTAAAACTGGCTTTAAATTGTTCTTTCCCTTCAGCGGGCCGTGACCCGCTCTCAGAGGCTATTTTAATAATGATAAAAGTATTGCAATGAAGAGCCGCATAGTGATTATGGAAAGAAGCTCTTAGCAAGGGCGAAAAGAGACTGTTCGTGTGTGTCAACAGGTATATCGTACGCTAAATGGCCAAAGATTTGAACGATTCGGGGTTTCCACCGAAGAGGAAGCCTTTGCTGCGTCCTCAACGATCTGATTTTACCGCAAATAGTTCGACAACTATGAACGTTAATGCAAACACAAGGGGGCGTGGTAGGCAGAAACAAGAGGGTGGTAAAGGCAGTTCGAGGTCACCGTCGTTACACTCTCCAAAATCATGGATAAGAAGCGCGTCTGCTACGGGGATCCTTGGACTAAGACGTCCCGAACTAGCACATTCTCATTCGCATGCTCCTTCCACCGGAACGCCTGCTGGAGGTAATCGTTCTCCTTTGAGAAGATCAACTGCAAATGCGACCCCCGTCGAGACCGGAAGGTCCTTGACCGACGGAGATATCAACAATGTTGTTGATGTACTGCCCTCATTTGAGATGTACAATACCCTGCATAGGCACATTCCGCAGGGCAACGTCGATCCAGATAGGCATGATTTCCCACCTTCATACCAAGAGGCCAATAATTCTACTGCAACAGGTGCTGCGGGCTCGAGCGCTGATCTCTCACATCAATCATTGTCCACTGACGCATTGGGTGCCACACGTTCTTCGTCAACATCAAATTTAGAAAACTTAATTCCCCTTCGAACCGAACATCACAGTATTGCAGCACATCAATCAACCGCTGTCGATGAAGATTCACTGGATATACCTCCCATACTTGATGACTTGAACGATACAGACAACATTTTCATCGACAAATTGTACACTTTACCAAAAATGTCCACACCCATCGAAATCACCATCAAGACGACGAAGCATGCACCTATACCACACGTGAAGCCGGAGGAGGAGTCCATTTTGAAAGAGTATACGTCGGGGGATTTGATTCATGGTTTTATCACTATTGAAAACAAATCTCAAGCAAACCTAAAGTTTGAAATGTTCTATGTCACTTTAGAGTCTTACATTTCCATTATTGATAAAGTAAAGAGTAAAAGAACGATTAAACGGTTTTTAAGGATGGTAGACTTGAGCGCTTCTTGGTCATACTCGAAAATAGCACTGGGGTCCGGTGTGGACTTCATTCCCGCAGATGTTGACTACGATGGTTCTGTATTTGGGCTAAACAATAGCCGGGTTCTGGAACCCGGAGTCAAGTACAAGAAATTCTTCATTTTCAAATTGCCACTGCAATTGCTAGATGTCACTTGTAAGCAGGAGCATTTCTCTCATTGTTTGTTACCTCCCAGTTTTGGTATTGATAAATATAGGAACAATTGCAAATATTCCGGTATCAAAGTCAATAGGGTACTTGGGTGCGGTCATTTAGGTACAAAGGGCTCCCCCATCTTAACTAACGATATGTCTGATGACAACCTTTCGATCAATTACACTATTGATGCAAGGATTGTCGGTAAAGATCAAAAGGCCTCTAAACTGTATATTATGAAGGAAAGAGAATATAATCTAAGAGTAATCCCTTTTGGTTTTGACGCCAATGTCGTCGGAGAAAGAACCACTATGAGTCAGCTGAATGATATCACCAAACTAGTGCAGGAAAGGTTGGATGCTCTTAGAAAAATCTTTCAGAGATTAGAGAAAAAAGAACCCATAACGAACCGCGACATTCACGGTGCAGACTTGAGTGGTACCATTGATGATTCTATTGAATCAGACTCCCAAGAAATTTTGCAGAGGAAATTGGACCAACTGCACATTAAGAACAGAAATAACTATTTAGTCAACTATAACGATTTGAAGTTGGGCCACGATTTGGACAATGGCCGCAGTGGAAATAGTGGTCATAATACCGATACTTCCAGAGCTTGGGGTCCCTTTGTTGAAAGTGAACTAAAATATAAACTGAAAAACAAATCCAATTCCTCCTCATTTCTGAACTTCTCTCATTTTTTAAACAGCAGTTCCAGCTCAATGTCCTCTTCCTCAAATGCGGGAAAGAATAATCATGATTTAACGGGAAATAAAGAAAGGACAGGGCTAATACTAGTAAAGGCGAAAATTCCAAAACAGGGCCTACCATATTGGGCTCCCTCATTATTGAGAAAGACCAATGTTTTTGAATCTAAGAGTAAACACGACCAAGAAAATTGGGTGAGATTGTCTGAGTTGATTCCGGAAGACGTAAAAAAACCATTGGAAAAACTTGATTTACAATTGACTTGCATAGAATCCGATAATAGCTTACCTCATGATCCGCCAGAAATTCAATCGATTACCACAGAACTGATATGTATAACTGCTAAATCTGATAATTCTATCCCAATAAAACTCAATTCTGAACTGTTGATGAACAAAGAGAAGCTGACAAGCATCAAAGCTTTGTACGATGATTTCCATTCAAAAATTTGTGAATATGAAACCAAGTTCAACAAGAATTTTCTTGAATTAAATGAGTTATATAATATGAATAGGGGAGACCGTAGGCCAAAGGAACTGAAATTTACAGATTTTATTACTTCACAGCTGTTTAACGATATCGAAAGCATTTGCAACTTGAAAGTTAGTGTTCACAACTTATCCAACATTTTTAAAAAACAGGTCAGTACCCTAAAACAACACTCAAAGCACGCATTATCTGAGGATTCAATATCGCACACAGGTAACGGTAGTTCATCGTCGCCCAGTTCAGCGTCATTAACGCCAGTAACTTCTTCATCCAAGAGTAGTTTATTTTTACCTAGCGGTAGCTCGTCTACTTCCCTGAAATTTACAGACCAGATTGTTCATAAATGGGTTAGGATTGCTCCTTTACAGTACAAACGAGACATTAATGTGAACTTGGAATTTAATAAGGACATTAAGGAAACTTTAATTCCAAGTTTTGAAAGCTGCCTATGTTGTAGGTTTTATTGCGTTCGAGTAATGATTAAATTTGAAAACCATCTTGGCGTAGCGAAGATTGATATCCCTATTTCTGTTAGGCAAGTGACAAAATAAAAAAACATTAGAAAAAATTCTCGTTACTTTTCTTATAGATATAGATATATGTATGGTTTGCTTATAGATGAAGGTATTTATCGCGTCCTTTGTATTCCCTATTATTAATAAAATTCTTTTAAAATGCATTTTCTGGTGCTCTTTTGTTGCTTCTGTATTTTTTTTTTTTTGGACCACTGGATGGAAAACCTTTGATGATTTTATTACCTTTATTTTAAGTTACTAAAATATCGAGATTTCAGGAACAAAACATAGAATTTTCTTTGTCAAGAAAAATAAAACGAAATAAATTGATGCTTTgaattc

Amino Acid Sequence

MAKDLNDSGFPPKRKPLLRPQRSDFTANSSTTMNVNANTRGRGRQKQEGGKGSSRSPSLHSPKSWIRSASATGILGLRRPELAHSHSHAPSTGTPAGGNRSPLRRSTANATPVETGRSLTDGDINNVVDVLPSFEMYNTLHRHIPQGNVDPDRHDFPPSYQEANNSTATGAAGSSADLSHQSLSTDALGATRSSSTSNLENLIPLRTEHHSIAAHQSTAVDEDSLDIPPILDDLNDTDNIFIDKLYTLPKMSTPIEITIKTTKHAPIPHVKPEEESILKEYTSGDLIHGFITIENKSQANLKFEMFYVTLESYISIIDKVKSKRTIKRFLRMVDLSASWSYSKIALGSGVDFIPADVDYDGSVFGLNNSRVLEPGVKYKKFFIFKLPLQLLDVTCKQEHFSHCLLPPSFGIDKYRNNCKYSGIKVNRVLGCGHLGTKGSPILTNDMSDDNLSINYTIDARIVGKDQKASKLYIMKEREYNLRVIPFGFDANVVGERTTMSQLNDITKLVQERLDALRKIFQRLEKKEPITNRDIHGADLSGTIDDSIESDSQEILQRKLDQLHIKNRNNYLVNYNDLKLGHDLDNGRSGNSGHNTDTSRAWGPFVESELKYKLKNKSNSSSFLNFSHFLNSSSSSMSSSSNAGKNNHDLTGNKERTGLILVKAKIPKQGLPYWAPSLLRKTNVFESKSKHDQENWVRLSELIPEDVKKPLEKLDLQLTCIESDNSLPHDPPEIQSITTELICITAKSDNSIPIKLNSELLMNKEKLTSIKALYDDFHSKICEYETKFNKNFLELNELYNMNRGDRRPKELKFTDFITSQLFNDIESICNLKVSVHNLSNIFKKQVSTLKQHSKHALSEDSISHTGNGSSSSPSSASLTPVTSSSKSSLFLPSGSSSTSLKFTDQIVHKWVRIAPLQYKRDINVNLEFNKDIKETLIPSFESCLCCRFYCVRVMIKFENHLGVAKIDIPISVRQVTK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59579
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast BUL1 (YMR275C) including 1000 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No