cDNA Clone CNF3

• Plasmid: pCMV5D CNF3 R190-end C865S
• Parent Plasmid: pCMV5D
• Expressed: CNF3 R190-end C865S
• Species: Human

Sequence of Insert

GAATTCATGCGGGATGATAATATAGAGTTGTTTTCCTTTGATATATATGGGTATTACTCTTCTGATATTTTCGGTGCTAAAAATGATGACAGAATAATGCTTTTCATTCCGGGCGCAACCAATCCTTTTTTATTCTCAGAAAATATTTCTCATTTGCGGACTCATCTTAAAGAATTAATAAAAGAAAATGATAATAGAGAATTACTGTCACGTCATTTTTCTCTGTATGACTGTCAGGATGGTTCTACTTTTTATGGTGTAGATTCGGTTCTCAAAGAAATAGTAAATGGAAACTTTAATGAGTCTTACTTCATGTACACCTATAAAAAATTCAATGAGCGGGATGTTTTTGATGCTATATCATTTTCCGTTCAGAAACGTAGTTTTAGTGATGGCGACACCATTATTAAATCAAACAGTGAAGCACAACGGGATTATGCACTGACAATAATCCAGGCAATCGTATCAATGATACCTGTGTTTGACATTATACTTCCGGAAGTGTCTGTTCCGCTTAGCATGGGTATTATAGCCTCCAGTATGGGGATCAGTTTTGATCAGCTAATTAATGGCGATACTTATGAAGAACGTCGTTCGGCAATTCCTGGAGTAGCGACAAATGCCGTATTGTTGGGTATATCCTTTGCACTCCCGTACCTGATTAGTAAGGCCAGTGAAAATAAGGTGATACTTAGTCAGACTGTAAGTAATGAAGACAGTATATTGAATGAAACAAATATTGATAATTTTCTTGCTGAAAATGGTATTAATAAAGACGATATACCTGCAAATGGAATATTAGAAGTTGATATAAAAAAATCCGGGATCCCTGTCAATCTTGTAAAAATTAGTGATGAAGACAATCAAATTGTTGCTGTCAGGGGAAGTTCTCAGAGTGGCATCTACTATGAAGTGGATATTGAAACAGGGTATGAGATTTTATCCCGAAGAGTTTACCGTACAGAATATAATAATGAAATATTCTGGATACGAAATGGCGGACTGAAAGGGGGACAACCATTCGATTTTGAAAATCTCGATATCCCTACTTTTTTCGTAGATAAACCTTATTCCGAACTGGCATCGTCTCCGGAACTGTCCTTTATCAATGATGACAGCCCACTATTGTTTCCTTATGTGGACTCAAGATTACCGAAACCAACGTCAGAAATGGATATCAGTTATTACTCATCTAATTTTTCCAGCTTTGCTGAGAATACTGTAACCCTGATGCGGGGGGCTACTGAAGAAGAAGCATGGAATATTGCCTATTATAAAACAGCAGGAGGAAGCAACAAAGAACTGGAAGAGATTTTTATAGGTGGAGGACCACAGGCTAATTTAAGTTTCACGGAATACACATCAAACATCAGAAGTGCAGATGCAGCAAGCAGAAGACATTTTCTTGTAGTCATAAACGTAAAAATAAAATATATCAGCAATGATAATGTTTTATATGCTAATCACTGGGCAATTCCTGATGAAGCCCCTGTTGAAGTGCTGGCGGTGGTCGACCGTAGATTTATATTTCCTGAACCTCCGACCCCACCAAAATTATCACTTATACAAAAAATATCACAAAGATTCTTTACAGAAGATATAGATGAAACATCCAGAATTAATTTTCAGAGATTAAACAGCGGTAATATTAATGTACTAAAAGGCAGGGGGAGTTTATCATCGAAAAATCAACGTTCAATTTACCTGCGTTTCGATGCCGTTAATGCTGATGATCTCCGACCAGATGAGATCTACGTCAAAAAAGACCAGTTTGATGACTTGGGTTATGATCGGTATTTCTATAATAACGCTGTGGGATTAGATGGCTCTCCCACACTGAACACTTATACTGGTGAATTTCTGACAGACCCATCTTTATTCGGTTCACTTTACTGGAGTAAATATAATCTGACCAATAAAACAAGCATTATTCGTGTAGCAAATTCAGCCAGGGGGGCAAACGGTATCAGAATAGCGCTTAAAGAAGTTCAGGAAAATAAACCTGTAATCATCACGAATGGAAATCTAAGTGGTTCTACCACGATCGTCGCACGAAAGGGAGAGTATCTGTATGAAGTGCATACTGGCACATTAGAGCCTCTGTTAGGTTTTACCAGTACAACAGGAGTAAAAAAAGCAGTGGAAGTTTTGTCGACACTCGCAGAACAAGAAATCCCTTCACTCGCGGGAACAATAAATAATGATTTTCTGGTAGATTTTTTAGCAGAAAACTTTGATAAATCATTAGTAACGTATTCATCATCAACACTAAAACCAGACAGCATAATTACCATTAGTCGTGATAATGTATCTACTTTTCCTTACTATACTGACGATATTATCCATCCAGGATTTGGCACATCGGTAACTATATTGGTGAGAATAGATGATAATACTGTTGTAAAGTCTCTGTCAGAAAGTTATGTTACAAATGCAGATGGCTCCAGGATATCAGTATTTAAGGTATTATCAAAAGATTTTTGAGCGGCCGC

Amino Acid Sequence

MRDDNIELFSFDIYGYYSSDIFGAKNDDRIMLFIPGATNPFLFSENISHLRTHLKELIKENDNRELLSRHFSLYDCQDGSTFYGVDSVLKEIVNGNFNESYFMYTYKKFNERDVFDAISFSVQKRSFSDGDTIIKSNSEAQRDYALTIIQAIVSMIPVFDIILPEVSVPLSMGIIASSMGISFDQLINGDTYEERRSAIPGVATNAVLLGISFALPYLISKASENKVILSQTVSNEDSILNETNIDNFLAENGINKDDIPANGILEVDIKKSGIPVNLVKISDEDNQIVAVRGSSQSGIYYEVDIETGYEILSRRVYRTEYNNEIFWIRNGGLKGGQPFDFENLDIPTFFVDKPYSELASSPELSFINDDSPLLFPYVDSRLPKPTSEMDISYYSSNFSSFAENTVTLMRGATEEEAWNIAYYKTAGGSNKELEEIFIGGGPQANLSFTEYTSNIRSADAASRRHFLVVINVKIKYISNDNVLYANHWAIPDEAPVEVLAVVDRRFIFPEPPTPPKLSLIQKISQRFFTEDIDETSRINFQRLNSGNINVLKGRGSLSSKNQRSIYLRFDAVNADDLRPDEIYVKKDQFDDLGYDRYFYNNAVGLDGSPTLNTYTGEFLTDPSLFGSLYWSKYNLTNKTSIIRVANSARGANGIRIALKEVQENKPVIITNGNLSGSTTIVARKGEYLYEVHTGTLEPLLGFTSTTGVKKAVEVLSTLAEQEIPSLAGTINNDFLVDFLAENFDKSLVTYSSSTLKPDSIITISRDNVSTFPYYTDDIIHPGFGTSVTILVRIDDNTVVKSLSESYVTNADGSRISVFKVLSKDF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU62460
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation F757
• Low Temp: No