cDNA Clone IRAK3

• Synonyms: Interleukin-1 receptor-associated kinase 3, ASRT5, IRAKM, IL-1 receptor-associated kinase M
• Plasmid: pCMV5 IRAK3
• Parent Plasmid: pCMV5 FLAG 1
• Expressed: IRAK3
• Genbank: NM_007199.2
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAAGCGGCCGC

Amino Acid Sequence

MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGLAERLSS
SWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQVLQEMGHRRAIH
LITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIPEHNEKGVLLK
SSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKM
QCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLF
DRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSVICGSISSANIL
LDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGK
LSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCL
SFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASL
YFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDR
MTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVP
SQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29757
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at G40 (c120t) and a SNP at I147V.; FLAG tag deleted from parent clone
• Low Temp: No