cDNA Clone GFP ULK1 kinase domain siRNA resistant

• Plasmid: pcDNA5 FT TO GFP-ULK1 kinase domain (E15-F277) siRNA resistant
• Parent Plasmid: pcDNA5 FT TO GFP-
• Expressed: GFP-ULK1 kinase domain (E15-F277) siRNA resistant
• Genbank: NM_003565.2 GI:225637564
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAGTTCTCCCGCAAGGACCTGATCGGCCACGGCGCCTTCGC
GGTGGTCTTCAAGGGCCGCCACCGCGAGAAGCACGATTTGGAGGTCGCCG
TCAAGTGCATTAACAAGAAGAACCTCGCCAAGTCTCAGACGCTGCTGGGG
AAGGAAATCAAAATCCTGAAGGAACTGAAACATGAAAACATCGTGGCCCT
GTACGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGTTATGGAGTACT
GCAACGGTGGGGACCTGGCCGACTACCTGCACGCCATGCGCACGCTGAGC
GAGGACACCATCAGGCTCTTCCTGCAGCAGATCGCGGGCGCCATGCGGCT
TCTGCACAGCAAAGGCATCATCCACCGCGACCTGAAACCGCAGAACATCC
TGCTGTCCAACCCCGCCGGCCGCCGCGCCAACCCCAACAGCATCCGCGTC
AAGATCGCTGACTTCGGCTTCGCTAGATATTTGCAATCGAACATGATGGC
GGCCACACTCTGCGGCTCCCCCATGTACATGGCCCCCGAGGTCATCATGT
CCCAGCACTACGACGGGAAGGCGGACCTGTGGAGCATCGGCACCATCGTC
TACCAGTGCCTGACGGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCAGGA
CCTGCGCCTGTTCTACGAGAAGAACAAGACGTTGGTCCCCACCATCCCCC
GGGAGACCTCGGCCCCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAACGC
AACCACAAGGACCGCATGGACTTCGATGAGTTTTTTCATCACCCTTTCTG
AGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMEFSRK
DLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKE
LKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFL
QQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFA
RYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKA
PFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDF
DEFFHHPF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29891
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: ULK1 kinase domain (Glu15-Phe277) ; siRNA resistant nt505-524 ; WT GCGCGGTACCTCCAGAGCAA > siRNA resistant GCTAGATATTTGCAATCGAA; Parental 41455
• Low Temp: No