cDNA Clone HIF1A (rat)

• Synonyms: MOP1
• Plasmid: pBabeD hygro FLAG rat HIF1A siRNA-resistant
• Parent Plasmid: pBabeD hygro FLAG
• Expressed: FLAG rat HIF1A siRNA-resistant
• Genbank: NM_024359.1 (with changes - see notes)
• Tag: FLAG
• Species: Rat

Sequence of Insert

GGATCCATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAATAGGATGAGTTCCGAACGTCGAAAAGAAAAGTCTAGGGATGCAGCACGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCCCACAACGTGAGCTCCCATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTACTTACGTGTGAGGAAACTTCTAGATGCTGGTGATCTTGACATTGAAGATGAAATGAAAGCACAGATGAACTGCTTTTATCTGAAAGCCCTGGATGGCTTTGTTATGGTGCTAACAGATGATGGTGACATGATTTACATTTCTGATAACGTGAACAAATACATGGGGTTGACTCAGTTTGAATTGACCGGTCATTCCGTCTTCGATTTTACCCATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAGGGGAAAGAACAAAACACGCAGCGAAGCTTTTTTCTCAGAATGAAATGTACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTCAGCAACGTGGAAGGTGCTGCACTGCACAGGCCACATTCATGTGTATGATACCAGCAGTAACCAGCCGCAGTGTGGCTACAAGAAACCGCCTATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCATCAAACATTGAAATTCCTTTAGACAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGATGAAAGGATTACTGAGTTGATGGGTTATGAGCCAGAAGAACTTTTGGGCCGTTCAATTTATGAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGACATGTTTACTAAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCAAAAAGAGGTGGATATGTCTGGGTTGAGACTCAAGCAACTGTTATATATAATACGAAGAACTCTCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATCTGTCCTCAAACCAGTTGAATCTTCAGATATGAAAATGACCCAGCTGTTCACTAAAGTGGAATCTGAGGACACGAGCTGCCTCTTCGACAAGCTTAAGAAAGAGCCCGATGCCCTGACTCTGCTAGCTCCAGCGGCTGGGGACACGATCATATCACTGGACTTCGGCAGCGATGACACGGAAACTGAAGACCAACAACTTGAAGATGTCCCGTTGTACAATGATGTAATGTTCCCCTCTTCTAATGAGAAATTAAATATAAATCTGGCAATGTCTCCATTACCTGCCTCTGAAACTCCAAAGCCACTTCGAAGTAGTGCTGATCCTGCACTGAATCAAGAGGTTGCATTGAAGTTAGAGTCAAGCCCAGAGTCACTGGGACTTTCTTTTACCATGCCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGCTCACCTGAGCCTAACAGTCCCAGTGAGTACTGCTTTGATGTGGACAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGAAAAACTGTTTGCTGAAGACACAGAAGCGAAGAATCCATTTTCAGCTCAGGACACTGATTTAGACTTGGAAATGCTGGCTCCCTATATCCCAATGGATGATGATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAGAGAGCAATTCTCCAAGCCCTCCGAGTGTGAGCACAGTTACAGGATTCCAGCAGACCCAGTTACAGAAACCTACCATCACTGTCACTGCCGCAACTGCCACCACCGCCACCACCACTGATGAATCAAAAGCAGTGACGAAGGACAATATAGAAGACATTAAAATACTGATTGCATCTCCACCTTCTACCCAAGTACCTCAAGAAATGACCACTGCTAAGGCATCAGCATACAGTGGTACTCACAGTCGGACAGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAAAAAACAGACAAAGCTCATCCAAGGAGCCTTAACCTATCTGTCACTTTGAATCAAAGAAATACTGTTCCTGAAGAAGAATTAAACCCAAAGACAATAGCTTTGCAGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCTCCCTTTTTCAAGCAGCAGGAATTGGAACGTTACTGCAGCAACCAGGTGACCGTGCCCCTACTATGTCGCTTTCTTGGAAACGAGTGAAAGGATACATATCTAGTGAACAGGATGGAATGGAGCAGAAGACAATTTTTTTAATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCAGTTACGATTGTGAAGTTAATGCTCCCATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQM
NCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSAT
WKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLT
KTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFD
KLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSF
TMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPS
PPSVSTVTGFQQTQLQKPTITVTAATATTATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTASPDRAGKRVIEKTDKAHPRSL
NLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDE
SGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63686
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: shRNA resistant to CTAACTGGACACAGTGTGTTT.; N.B. Changes compared with reference:; residue change G75D; Additional A residue after A613; Additional T residue after T616; Additional T residue after A617; (Derived from RNA template. All common variants seen in other rat HIF1A sequences)
• Low Temp: No