cDNA Clone NEK10

• Plasmid: pBabeD puro Nek10 (long) K548R D673A 3FLAG
• Parent Plasmid: pBabeD puro C-3FLAG
• Expressed: NEK10 tv 2 (long) K548R D673A 3FLAG
• Genbank: NM_152534.4 with 6 silent changes
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGCCTGATCAAGATAAAAAGGTGAAGACCACAGAAAAATCAACTGATAAACAGCAAGAAATCACCATCAGGGACTATTCAGATCTTAAAAGACTTCGGTGCCTTTTGAACGTCCAATCAAGCAAACAACAGCTTCCAGCCATTAACTTCGATAGTGCCCAAAATAGCATGACGAAGTCTGAGCCCGCCATCAGGGCGGGTGGACACAGAGCTCGGGGTCAGTGGCATGAATCCACAGAAGCTGTTGAACTTGAAAATTTTAGTATAAACTACAAGAATGAGAGAAATTTCAGCAAACATCCTCAGCGTAAACTATTTCAGGAGATCTTTACCGCCTTGGTGAAAAATAGACTCATAAGCAGAGAGTGGGTTAATCGAGCCCCATCTATTCATTTTCTGAGAGTGTTAATCTGTCTGAGGCTACTAATGAGGGACCCATGTTATCAGGAAATACTCCATAGCTTGGGTGGGATTGAAAACCTAGCTCAGTATATGGAGATTGTAGCCAATGAGTACCTCGGCTATGGAGAAGAGCAGCACACTGTGGACAAGCTGGTCAACATGACATATATTTTTCAAAAACTTGCTGCAGTCAAAGATCAAAGAGAATGGGTCACCACAAGTGGAGCCCACAAGACATTAGTAAATTTACTTGGTGCCCGAGATACTAATGTTCTATTGGGTTCCCTTCTGGCTCTGGCTAGTTTAGCAGAAAGTCAAGAATGTAGGGAGAAGATAAGTGAACTCAACATTGTAGAAAATCTGTTGATGATTTTACATGAATATGACTTGCTTTCTAAAAGACTAACAGCGGAGTTGCTGCGCCTACTTTGTGCAGAGCCCCAGGTGAAAGAGCAGGTGAAGCTCTATGAGGGGATACCGGTCCTCCTCAGTCTGCTCCACTCTGACCACTTGAAGCTCCTCTGGAGCATTGTCTGGATTCTGGTACAGGTTTGTGAGGACCCTGAGACCAGCGTGGAAATTCGCATTTGGGGAGGCATCAAACAGCTTCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGATCACTCCTCCATTGGAAGCCTGTCCAGTGCAAATGCTGCAGGCCGAATCCAGCAGCTTCATTTATCAGAAGACTTGAGCCCTAGGGAAATACAAGAAAATACTTTCTCACTTCAAGCAGCCTGCTGTGCTGCCCTCACTGAGCTGGTGCTCAATGACACCAATGCCCACCAGGTGGTTCAGGAAAATGGTGTATATACAATAGCAAAATTAATTTTACCAAATAAGCAAAAGAATGCAGCAAAAAGTAATCTATTACAGTGTTATGCTTTCAGAGCCTTGAGATTTCTCTTCAGTATGGAAAGAAACAGACCACTCTTTAAAAGACTTTTCCCCACAGACTTGTTTGAGATCTTCATTGACATAGGGCATTATGTACGTGATATCAGTGCTTATGAAGAATTGGTATCCAAGCTGAATTTATTAGTGGAGGATGAACTGAAGCAAATTGCTGAAAATATTGAAAGCATTAATCAGAACAAAGCTCCTTTGAAATATATAGGCAACTATGCAATTTTGGATCATCTTGGAAGTGGAGCTTTTGGCTGTGTTTACAAGGTTAGAAAGCATAGTGGTCAAAATCTTTTAGCAATGAGAGAGGTCAATTTACATAACCCAGCATTTGGGAAGGATAAGAAAGATCGAGACAGCAGCGTAAGGAATATTGTTTCTGAATTAACAATAATTAAAGAGCAGCTTTATCATCCCAACATTGTACGTTATTACAAAACATTTCTGGAAAATGATAGGTTGTACATAGTTATGGAGCTGATAGAAGGAGCCCCGCTTGGAGAGCATTTCAGTTCTTTGAAGGAAAAACATCACCATTTTACTGAAGAAAGACTATGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGATACTTACACAAGGAGAAGAGGATTGTCCATAGAGATCTGACACCAAACAACATTATGTTGGGGGATAAGGACAAAGTAACCGTTACTGCCTTTGGCCTGGCAAAGCAAAAACAAGAAAACAGTAAACTCACGTCTGTGGTTGGAACAATCCTGTATTCTTGCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATGTCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCTGTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTAACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGATGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCTAGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCATGAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCAGCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGACGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACATATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAGAATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGACTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCACCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCCTTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGGGCCACAGATGGGCACATTCTTGTGGCAAGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGTGCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTTCCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGCAGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCCCAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGACCCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTGAAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGACCAAGAATCACCCAACCAAAAGAGCGGCCGC

Amino Acid Sequence

MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKH
PQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKD
QREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLL
HSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVL
NDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIA
ENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMREVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRL
YIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTAFGLAKQKQENSKLTSVVGTILYSCPEV
LKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLS
LEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYS
EVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNL
KDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTA
DYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKRAAADYKDHDGDYKDHDIDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63785
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No