cDNA Clone GABARAPL2

• Plasmid: pBabeD puro FLAG-BFP-GABARAPL2 KO BamHI
• Parent Plasmid: pBabeD puro FLAG
• Expressed: FLAG-BFP-GABARAPL2
• Genbank: NP_009216.1 NM_007285.6 GI:27374999
• Tag: FLAG
• Tag: BFP
• Species: Human

Sequence of Insert

agatctATGAGCGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACAT
GGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAAG
GCAAGCCCTACGAGGGCACCCAGACCATGAGAATCAAGGTGGTCGAGGGC
GGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACTAGCTTCCTCTACGG
CAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTCAAGC
AGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATACGAAGAC
GGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCT
CATCTACAACGTCAAGATCAGAGGGGTGAACTTCACATCCAACGGCCCTG
TGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAGACGCTGTAC
CCCGCTGACGGCGGCCTGGAAGGCAGAAACGACATGGCCCTGAAGCTCGT
GGGCGGGAGCCATCTGATCGCAAACATCAAGACCACATATAGATCCAAGA
AACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATGTGGACTACAGA
CTGGAAAGAATCAAGGAGGCCAACAACGAGACCTACGTCGAGCAGCACGA
GGTGGCAGTGGCCAGATACTGCGACCTCCCTAGCAAACTGGGGCACAAGC
TTAATggatcc

Amino Acid Sequence

MDYKDDDDKGSMSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQ
TMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFT
WERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLG
WEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKM
PGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNGSMKWM
FKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSD
ITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGF
LYVAYSGENTFGF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Vector 65044 (Parental 37983). Internal BamHI site (at R67 of GABARAPL2 sequence) knocked out silently
• Low Temp: No