cDNA Clone GFP ATG2A

• Plasmid: pBabe puro GFP ATG2A S1826D
• Parent Plasmid: pBABE puro GFP
• Expressed: GFP ATG2A S1826D
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCACGATGGCTGTGGCCATGGTCAAACTGTGTGAAAGAGCG
GGTCTGCCGCTACTTGCTGCACCACTACTTAGGTCACTTCTTCCAAGAGC
ACCTCAGCCTGGACCAGCTCAGCCTCGATCTGTACAAGGGCAGCGTTGCC
CTGCGAGACATCCACCTGGAAATCTGGTCTGTGAACGAGGTGCTGGAGTC
AATGGAGTCACCGCTGGAGCTGGTGGAAGGCTTCGTGGGCTCCATCGAGG
TGGCCGTGCCCTGGGCTGCTCTGCTCACCGACCACTGCACAGTGCGCGTG
TCCGGCCTCCAGCTCACCTTGCAGCCCCGCCGGGGTCCAGCGCCAGGGGC
TGCCGACTCACAGAGCTGGGCCTCATGCATGACCACAAGCCTGCAGCTGG
CCCAGGAGTGTCTGCGGGATGGGCTACCGGAGCCCTCTGAGCCACCACAG
CCCCTGGAGGGGCTGGAGATGTTTGCCCAGACCATTGAGACTGTGCTTCG
GAGGATCAAAGTGACCTTCCTGGACACTGTCGTGAGGGTGGAGCACTCTC
CGGGTGATGGGGAACGTGGTGTGGCCGTCGAGGTCCGTGTGCAGAGACTG
GAGTACTGTGATGAGGCAGTGCGGGACCCAAGCCAGGCGCCGCCGGTGGA
TGTGCATCAGCCGCCTGCCTTCCTGCACAAGCTGCTGCAGCTGGCAGGGG
TCCGCCTGCACTACGAGGAGCTCCCGGCACAGGAAGAGCCTCCAGAGCCC
CCCTTGCAGATCGGCAGCTGCTCAGGGTACATGGAGCTGATGGTGAAGTT
GAAGCAAAATGAGGCCTTCCCTGGCCCCAAGTTGGAGGTGGCGGGACAGC
TGGGCTCCCTGCACCTGCTCCTGACCCCGAGGCAGCTCCAGCAACTTCAG
GAACTGCTCAGCGCCGTGAGCCTTACAGACCACGAGGGCCTGGCTGACAA
GCTGAACAAGAGCCGCCCGCTAGGTGCCGAAGACCTGTGGCTGATTGAGC
AGGACCTGAACCAGCAGCTGCAGGCAGGGGCAGTGGCTGAGCCCCTCAGC
CCAGACCCCCTTACCAACCCCCTTCTCAACCTGGATAACACTGACCTCTT
CTTCTCCATGGCTGGCCTCACAAGCAGTGTGGCCTCAGCCCTCTCTGAGC
TCTCCCTCTCCGATGTAGACCTGGCCTCCTCTGTGCGCAGCGACATGGCC
TCCCGCCGGCTCTCTGCCCAGGCCCACCCAGCTGGCAAGATGGCCCCCAA
CCCCCTCCTGGACACCATGCGCCCTGACTCGCTGCTGAAGATGACCTTGG
GGGGTGTGACCCTGACCTTGCTTCAGACGTCTGCCCCATCTTCCGGACCA
CCTGACCTCGCCACGCACTTTTTCACCGAGTTTGATGCCACCAAGGATGG
GCCCTTCGGTTCCCGAGACTTCCATCACCTTCGACCACGCTTCCAGAGGG
CCTGTCCCTGTAGCCATGTTCGGCTAACGGGCACAGCCGTGCAGCTGTCC
TGGGAGCTGCGGACGGGCAGTCGGGGCCGGCGGACAACCAGCATGGAAGT
GCACTTCGGGCAGCTGGAGGTGCTGGAGTGTCTGTGGCCCCGGGGCACCT
CTGAGCCTGAGTACACGGAGATCCTGACCTTTCCTGGTACGCTGGGCTCC
CAGGCCTCAGCTCGGCCCTGCGCCCATCTGCGCCACACACAGATCCTGCG
CCGTGTGCCTAAGAGCCGACCCCGGCGCTCAGTTGCCTGCCATTGCCACT
CAGAACTGGCCCTGGACCTGGCCAACTTCCAGGCGGACGTGGAGCTGGGG
GCCCTGGACCGGCTGGCCGCCCTACTGCGCCTGGCCACCGTACCTGCTGA
GCCTCCAGCCGGCCTGCTGACAGAGCCCCTGCCGGCGATGGAGCAGCAGA
CGGTATTTCGGCTCTCTGCACCCCGGGCCACGCTGCGGCTGCGCTTCCCC
ATTGCCGACCTGCGGCCTGAGCGGGACCCCTGGGCGGGCCAGGCCGTGCG
GGCTGAGCAGCTTCGGCTGGAGCTGAGTGAGCCCCAGTTCCGGTCAGAGC
TTAGCAGTGGGCCTGGTCCCCCAGTCCCCACCCACCTGGAACTCACCTGC
TCCGACCTACATGGTATCTATGAAGATGGAGGGAAGCCACCTGTCCCTTG
CCTGCGTGTCTCCAAAGCCCTGGACCCCAAGAGCACTGGGCGCAAGTACT
TCCTGCCCCAGGTAGTGGTGACTGTGAACCCCCAGTCCAGCAGCACACAG
TGGGAGGTGGCCCCGGAGAAGGGAGAGGAACTGGAGCTGTCAGTGGAGAG
TCCCTGTGAGCTGCGGGAACCTGAGCCCTCGCCCTTCTCCTCTAAGAGGA
CCATGTATGAGACAGAGGAGATGGTGATCCCTGGAGACCCTGAGGAGATG
AGGACGTTCCAGAGCCGGACCCTGGCACTGTCCCGCTGCAGCCTGGAAGT
GATCCTGCCCAGTGTCCACATCTTTCTGCCCAGCAAGGAGGTCTACGAGA
GCATCTACAACAGGATCAACAACGACCTGCTCATGTGGGAGCCTGCAGAT
CTGCTTCCCACCCCCGACCCCGCCGCCCAGCCCTCGGGCTTCCCCGGCCC
CTCAGGCTTCTGGCACGACAGCTTTAAGATGTGCAAGTCAGCCTTCAAGC
TGGCCAACTGCTTTGATCTCACCCCAGACTCGGACTCGGATGACGAGGAT
GCCCACTTCTTCTCAGTGGGGGCATCAGGTGGCCCACAGGCCGCTGCCCC
TGAGGCCCCAAGTCTTCACTTGCAGAGCACCTTCTCTACACTGGTGACAG
TGCTGAAGGGGCGGATCACAGCCCTCTGTGAGACCAAGGATGAGGGTGGG
AAGCGGCTGGAGGCTGTGCACGGGGAGCTGGTGCTGGACATGGAGCACGG
TACCCTCTTCAGCGTCTCCCAGTACTGTGGCCAGCCAGGACTTGGCTACT
TCTGTCTGGAAGCTGAAAAGGCAACACTCTACCACCGAGCGGCCGTGGAT
GACTACCCGCTGCCCAGTCACCTGGACCTTCCCAGTTTCGCTCCCCCGGC
TCAGCTGGCCCCAACCATCTACCCATCGGAGGAAGGGGTGACCGAGCGGG
GAGCCTCGGGCCGCAAGGGCCAGGGCCGGGGACCCCACATGTTGTCCACT
GCTGTGCGCATCCACCTGGACCCCCACAAGAATGTGAAGGAGTTCCTGGT
GACACTGCGGTTGCACAAAGCCACCTTGCGCCACTACATGGCCCTGCCCG
AGCAGAGCTGGCATTCCCAGTTGTTGGAGTTCCTAGACGTGCTGGATGAC
CCTGTGCTGGGCTACCTGCCCCCGACGGTCATCACCATCCTGCACACACA
CCTGTTCTCCTGCTCTGTGGACTATAGGCCACTCTACCTCCCAGTGCGTG
TCCTCATCACCGCGGAGACCTTCACTCTCTCCAGCAACATCATCATGGAC
ACCTCCACCTTCCTGCTCAGGTTCATCCTCGATGACTCCGCCTTGTACCT
GTCCGACAAGTGTGAGGTGGAGACCCTGGACCTGCGGCGAGATTATGTCT
GTGTTTTGGATGTTGACCTCTTGGAACTTGTGATTAAAACCTGGAAAGGG
AGCACCGAGGGCAAACTGAGCCAGCCACTATTCGAGCTGCGCTGCTCCAA
CAATGTGGTACACGTGCACAGCTGTGCCGACTCCTGTGCCCTGCTGGTCA
ACCTGCTCCAGTACGTAATGAGCACAGGCGATCTGCACCCCCCACCCCGG
CCCCCCAGCCCCACGGAGATCGCCGGCCAGAAGCTCTCGGAGAGTCCTGC
CTCTCTGCCCTCGTGCCCCCCAGTGGAGACGGCCCTCATCAACCAGCGTG
ACCTGGCCGACGCCCTCCTGGACACCGAGCGCAGCCTACGGGAGCTGGCC
CAGCCTTCAGGTGGCCACCTCCCTCAGGCGTCGCCCATCTCCGTCTACCT
ATTCCCAGGTGAACGGAGTGGGGCCCCACCCCCTTCACCACCTGTCGGGG
GCCCTGCTGGCAGCTTAGGGTCATGCTCAGAGGAGAAGGAAGATGAAAGG
GAAGAGGAGGGCGATGGAGACACCCTGGACAGTGATGAGTTCTGCATCCT
TGATGCTCCCGGCCTGGGCATCCCGCCCCGAGATGGGGAGCCTGTGGTGA
CACAGCTGCATCCCGGCCCCATCGTTGTGAGGGACGGTTACTTCTCACGG
CCGATCGGCAGCACGGACTTGCTGCGGGCACCTGCCCATTTCCCAGTGCC
CAGCACTCGGGTGGTGCTACGTGAGGTCTCCCTCGTCTGGCACCTCTATG
GGGGCCGAGACTTTGGCCCCCACCCCGGCCACAGGGCAAGAACTGGCCTC
TCAGGTCCCAGGAGCTCCCCTTCCCGCTGCTCTGGCCCCAACCGGCCCCA
GAACTCATGGCGCACGCAGGGGGGCAGCGGGCGGCAGCACCATGTCCTCA
TGGAGATCCAGCTGAGCAAGGTAAGCTTCCAGCACGAGGTGTACCCAGCG
GAGCCAGCCACAGGCCCTGCGGCCCCCAGCCAGGAGCTGGAGGAGCGACC
GCTGTCCCGTCAGGTGTTCATCGTGCAGGAGCTGGAGGTCCGAGACCGGC
TCGCCTCCTCCCAGATCAACAAGTTCCTGTACCTACACACGAGTGAGCGG
ATGCCGCGACGTGCCCACTCTAACATGCTCACCATCAAAGCGCTGCATGT
GGCCCCCACTACCAACCTGGGTGGGCCTGAGTGCTGTCTCCGCGTCTCGC
TGATGCCCCTGCGGCTCAATGTGGACCAGGATGCCCTCTTCTTCCTCAAG
GACTTCTTCACTAGTCTGGTGGCCGGCATCAACCCCGTGGTCCCAGGGGA
GACCTCCGCTGAGGCTCGCCCCGAGACTCGAGCCCAGCCCAGCAGCCCCC
TGGAAGGGCAGGCCGAAGGCGTAGAGACCACTGGTTCGCAGGAGGCCCCA
GGAGGTGGACACAGCCCCTCCCCTCCTGACCAGCAGCCCATCTACTTCAG
AGAGTTCCGCTTCACGTCTGAGGTCCCCATCTGGCTGGATTACCATGGCA
AGCACGTCACGATGGACCAGGTGGGCACTTTTGCTGGCCTCCTCATCGGC
CTGGCCCAACTCAACTGCTCCGAGCTGAAGCTAAAGCGGCTCTGTTGCAG
GCACGGGCTCCTGGGTGTGGACAAGGTGCTGGGCTATGCCCTCAACGAGT
GGCTGCAGGACATCCGCAAGAACCAGCTGCCCGGCCTGCTGGGAGGCGTG
GGCCCCATGCACTCGGTTGTCCAGCTCTTCCAAGGGTTCCGGGACCTGCT
GTGGCTGCCCATTGAGCAGTACAGGAAGGATGGCCGCCTCATGCGGGGGC
TGCAGCGAGGGGCTGCCTCCTTTGGCTCATCCACAGCCTCTGCCGCCCTG
GAACTCAGCAACCGGTTGGTACAGGCTATCCAGGCCACAGCTGAGACCGT
GTATGACATCCTGTCCCCGGCAGCCCCCGTCgaCCGCTCCCTGCAGGATA
AGCGCTCTGCGCGGAGGCTGCGCAGGGGCCAGCAGCCTGCCGACCTGCGG
GAGGGTGTGGCCAAGGCCTACGACACAGTGCGAGAGGGCATCTTGGATAC
AGCTCAGACCATCTGTGACGTGGCATCGCGGGGCCATGAGCAGAAGGGGC
TGACGGGCGCCGTGGGGGGCGTGATCCGCCAGCTGCCCCCGACTGTGGTG
AAGCCGCTCATCCTGGCCACGGAGGCCACGTCCAGCCTGCTCGGGGGCAT
GCGCAACCAGATTGTCCCCGACGCCCACAAGGACCACGCCCTCAAGTGGC
GCTCGGACAGTGCCCAAGACTGAGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSRWLW
PWSNCVKERVCRYLLHHYLGHFFQEHLSLDQLSLDLYKGSVALRDIHLEI
WSVNEVLESMESPLELVEGFVGSIEVAVPWAALLTDHCTVRVSGLQLTLQ
PRRGPAPGAADSQSWASCMTTSLQLAQECLRDGLPEPSEPPQPLEGLEMF
AQTIETVLRRIKVTFLDTVVRVEHSPGDGERGVAVEVRVQRLEYCDEAVR
DPSQAPPVDVHQPPAFLHKLLQLAGVRLHYEELPAQEEPPEPPLQIGSCS
GYMELMVKLKQNEAFPGPKLEVAGQLGSLHLLLTPRQLQQLQELLSAVSL
TDHEGLADKLNKSRPLGAEDLWLIEQDLNQQLQAGAVAEPLSPDPLTNPL
LNLDNTDLFFSMAGLTSSVASALSELSLSDVDLASSVRSDMASRRLSAQA
HPAGKMAPNPLLDTMRPDSLLKMTLGGVTLTLLQTSAPSSGPPDLATHFF
TEFDATKDGPFGSRDFHHLRPRFQRACPCSHVRLTGTAVQLSWELRTGSR
GRRTTSMEVHFGQLEVLECLWPRGTSEPEYTEILTFPGTLGSQASARPCA
HLRHTQILRRVPKSRPRRSVACHCHSELALDLANFQADVELGALDRLAAL
LRLATVPAEPPAGLLTEPLPAMEQQTVFRLSAPRATLRLRFPIADLRPER
DPWAGQAVRAEQLRLELSEPQFRSELSSGPGPPVPTHLELTCSDLHGIYE
DGGKPPVPCLRVSKALDPKSTGRKYFLPQVVVTVNPQSSSTQWEVAPEKG
EELELSVESPCELREPEPSPFSSKRTMYETEEMVIPGDPEEMRTFQSRTL
ALSRCSLEVILPSVHIFLPSKEVYESIYNRINNDLLMWEPADLLPTPDPA
AQPSGFPGPSGFWHDSFKMCKSAFKLANCFDLTPDSDSDDEDAHFFSVGA
SGGPQAAAPEAPSLHLQSTFSTLVTVLKGRITALCETKDEGGKRLEAVHG
ELVLDMEHGTLFSVSQYCGQPGLGYFCLEAEKATLYHRAAVDDYPLPSHL
DLPSFAPPAQLAPTIYPSEEGVTERGASGRKGQGRGPHMLSTAVRIHLDP
HKNVKEFLVTLRLHKATLRHYMALPEQSWHSQLLEFLDVLDDPVLGYLPP
TVITILHTHLFSCSVDYRPLYLPVRVLITAETFTLSSNIIMDTSTFLLRF
ILDDSALYLSDKCEVETLDLRRDYVCVLDVDLLELVIKTWKGSTEGKLSQ
PLFELRCSNNVVHVHSCADSCALLVNLLQYVMSTGDLHPPPRPPSPTEIA
GQKLSESPASLPSCPPVETALINQRDLADALLDTERSLRELAQPSGGHLP
QASPISVYLFPGERSGAPPPSPPVGGPAGSLGSCSEEKEDEREEEGDGDT
LDSDEFCILDAPGLGIPPRDGEPVVTQLHPGPIVVRDGYFSRPIGSTDLL
RAPAHFPVPSTRVVLREVSLVWHLYGGRDFGPHPGHRARTGLSGPRSSPS
RCSGPNRPQNSWRTQGGSGRQHHVLMEIQLSKVSFQHEVYPAEPATGPAA
PSQELEERPLSRQVFIVQELEVRDRLASSQINKFLYLHTSERMPRRAHSN
MLTIKALHVAPTTNLGGPECCLRVSLMPLRLNVDQDALFFLKDFFTSLVA
GINPVVPGETSAEARPETRAQPSSPLEGQAEGVETTGSQEAPGGGHSPSP
PDQQPIYFREFRFTSEVPIWLDYHGKHVTMDQVGTFAGLLIGLAQLNCSE
LKLKRLCCRHGLLGVDKVLGYALNEWLQDIRKNQLPGLLGGVGPMHSVVQ
LFQGFRDLLWLPIEQYRKDGRLMRGLQRGAASFGSSTASAALELSNRLVQ
AIQATAETVYDILSPAAPVDRSLQDKRSARRLRRGQQPADLREGVAKAYD
TVREGILDTAQTICDVASRGHEQKGLTGAVGGVIRQLPPTVVKPLILATE
ATSSLLGGMRNQIVPDAHKDHALKWRSDSAQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains SNP P656R
• Low Temp: No