cDNA Clone DCAF14

• Synonyms: BRWD2; DCAF14; DIDOD; ndrp; WDR11
• Plasmid: pcDNA5D FRT/TO puro mScarlet PHIP (DCAF14)
• Parent Plasmid: pcDNA5D FRT/TO puro mScarlet
• Expressed: mScarlet PHIP (DCAF14)
• Genbank: NM_017934.6 (with changes - see notes)
• Tag: mScarlet
• Species: Human

Sequence of Insert

GGATCCATGTCTTGTGAGAGGAAAGGCCTCTCGGAGCTGCGATCGGAGCTCTACTTCCTCATCGCCCGGTTCCTGGAAGATGGACCCTGTCAGCAGGCGGCTCAGGTGCTGATCCGCGAGGTGGCCGAGAAGGAGCTGCTGCCCCGGCGCACCGACTGGACCGGGAAGGAGCATCCCAGGACCTACCAGAATCTGGTGAAGTATTACAGACACTTAGCACCTGATCACTTGCTGCAAATATGTCATCGACTAGGACCTCTTCTTGAACAAGAAATTCCTCAAAGTGTTCCTGGAGTACAAACTTTATTAGGAGCTGGAAGACAGTCTTTACTACGCACAAATAAAAGCTGCAAGCATGTTGTGTGGAAAGGATCTGCTCTGGCTGCGTTGCACTGTGGAAGACCACCTGAGTCACCAGTTAACTATGGTAGCCCACCCAGCATTGCGGATACTCTGTTTTCAAGGAAGCTGAATGGGAAATACAGACTTGAGCGACTTGTTCCAACTGCAGTGTATCAGCACATGAAAATGCATAAACGAATTCTTGGACACTTGTCATCTGTGTACTGTGTAACTTTTGATCGAACTGGCAGACGGATATTTACTGGTTCTGATGACTGTCTTGTGAAAATATGGGCAACAGATGATGGGAGGTTGTTAGCTACCTTAAGAGGACATGCTGCTGAAATATCAGACATGGCTGTAAACTATGAGAATACCATGATAGCAGCTGGAAGTTGTGATAAAATGATCCGAGTCTGGTGTCTTCGAACCTGTGCACCTTTGGCTGTTCTTCAGGGCCATAGTGCATCTATTACATCACTACAGTTCTCACCATTGTGCAGTGGCTCAAAGAGATATCTATCTTCTACTGGGGCAGATGGCACTATTTGTTTTTGGCTCTGGGATGCTGGAACCCTTAAAATAAACCCAAGACCTGCAAAATTTACAGAGCGCCCTCGGCCTGGAGTTCAAATGATCTGTTCTTCTTTTAGTGCTGGTGGAATGTTTCTGGCGACGGGAAGCACAGATCATATTATTCGGGTTTATTTTTTTGGATCAGGTCAGCCAGAGAAAATATCAGAATTGGAGTTTCATACTGACAAAGTTGACAGTATCCAGTTTTCCAACACTAGTAACAGGTTTGTAAGTGGCAGTCGTGATGGGACAGCACGTATTTGGCAATTTAAACGAAGAGAGTGGAAGAGCATTTTGTTGGATATGGCTACTCGTCCAGCAGGCCAAAACCTTCAAGGAATAGAAGATAAAATCACAAAAATGAAGGTTACTATGGTAGCTTGGGATCGACATGACAATACAGTTATAACTGCAGTTAATAACATGACTCTGAAAGTTTGGAATTCTTACACTGGTCAACTAATTCATGTCCTGATGGGTCATGAAGATGAGGTATTTGTTCTTGAACCACACCCGTTCGATCCTAGAGTTCTCTTTTCTGCTGGTCATGATGGAAACGTGATAGTGTGGGATCTGGCAAGAGGAGTCAAAATACGATCTTATTTCAATATGATTGAAGGCCAAGGACATGGCGCAGTATTTGACTGCAAATGCTCTCCTGATGGTCAGCATTTTGCATGCACAGACTCTCATGGACATCTTTTAATTTTTGGCTTTGGGTCCAGTAGCAAATATGACAAGATAGCAGATCAGATGTTCTTTCATAGTGATTATCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGAACAGACTCAGCAAGCACCTCATCTTATGCCTCCCCCTTTTTTGGTTGATGTTGATGGTAACCCTCATCCATCAAGATATCAAAGATTAGTTCCTGGCCGTGAAAATTGCAGGGAGGAGCAACTCATCCCTCAGATGGGAGTAACTTCCTCAGGACTGAATCAAGTTTTAAGTCAGCAAGCAAACCAGGAGATCAGCCCACTGGACAGCATGATTCAAAGACTACAACAGGAGCAAGACCTGAGACGTTCTGGTGAAGCAGGTATCAGTAATACCAGCCGTTTAAGTAGAGGCTCCATAAGTTCTACCTCAGAGGTTCATTCACCACCAAACGTAGGACTAAGACGTAGTGGACAAATTGAAGGTGTACGGCAAATGCACAGCAACGCACCAAGAAGTGAAATAGCCACAGAGCGGGATCTGGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCCAGTAGGCAAGAAGAATGGAGAACTGCAAAGGGAGAAGAAGAAATAAAGACTTACAGGTCAGAAGAGAAAAGAAAACACTTAACTGTTCCAAAAGAGAATAAAATACCCACTGTCTCAAAGAATCATGCTCATGAGCATTTCCTGGATCTTGGAGAATCCAAAAAGCAACAGACAAATCAACACAATTATCGTACAAGATCTGCATTGGAAGAGACTCCTAGACCCTCAGAAGAGATAGAAAATGGCAGTAGTTCTTCAGATGAAGGCGAAGTAGTTGCTGTCAGTGGTGGAACATCCGAAGAAGAAGAGAGAGCATGGCACAGTGATGGCAGTTCTAGTGACTACTCCAGTGATTACTCTGACTGGACAGCAGATGCAGGAATTAATCTGCAGCCACCAAAGAAAGTTCCTAAGAATAAAACCAAGAAAGCAGAAAGCAGTTCAGATGAAGAAGAAGAATCTGAAAAACAGAAGCAAAAACAGATTAAAAAGGAAAAGAAAAAAGTAAATGAAGAAAAAGATGGACCAATATCACCAAAGAAAAAGAAGCCCAAAGAAAGAAAACAAAAGAGATTGGCTGTGGGAGAACTAACTGAAAATGGTTTGACATTAGAAGAATGGTTGCCATCAACGTGGATTACAGATACCATTCCCCGAAGATGTCCATTTGTGCCACAGATGGGTGATGAGGTTTATTATTTCCGACAAGGACATGAAGCCTATGTCGAAATGGCCCGGAAAAATAAAATATATAGTATCAATCCCAAAAAACAACCATGGCATAAAATGGAGCTACGGGAACAAGAACTTATGAAAATAGTTGGCATAAAGTATGAAGTGGGATTACCTACCCTTTGCTGCCTTAAACTTGCTTTTCTAGATCCTGATACTGGTAAACTGACTGGCGGATCATTTACCATGAAATACCATGATATGCCTGACGTTATAGATTTCCTAGTCTTGAGACAACAATTTGATGATGCAAAATACAGGCGATGGAATATAGGTGACCGCTTCAGGTCTGTCATAGATGATGCCTGGTGGTTTGGAACAATCGAAAGCCAGGAACCTCTTCAACCTGAGTACCCTGATAGTCTGTTTCAATGCTACAATGTTTGCTGGGACAATGGAGATACAGAAAAGATGAGTCCTTGGGATATGGAGCTTATACCTAATAATGCTGTATTTCCTGAAGAACTAGGTACCAGTGTTCCTTTAACTGATGGTGAGTGCAGATCACTAATCTATAAACCTCTTGATGGAGAATGGGGTACCAATCCCAGGGATGAAGAATGTGAAAGAATTGTGGCAGGAATAAACCAGTTGATGACCCTAGATATTGCCTCAGCATTTGTGGCCCCCGTGGATCTGCAAGCCTATCCCATGTATTGCACAGTAGTGGCATATCCAACGGATCTAAGTACAATTAAACAAAGACTGGAAAACAGGTTTTACAGGCGGGTTTCTTCCCTAATGTGGGAAGTTCGATATATAGAGCATAATACACGAACATTTAATGAGCCTGGAAGCCCTATTGTGAAATCTGCTAAATTCGTGACTGATCTTCTTCTACATTTTATAAAGGATCAGACTTGTTATAACATAATTCCACTTTATAATTCAATGAAGAAGAAAGTTTTGTCTGATTCTGAGGATGAAGAGAAAGATGCTGATGTGCCAGGAACTTCTACTCGAAAAAGGAAGGACCATCAGCCTAGAAGAAGATTACGTAATAGAGCCCAGTCTTACGATATTCAAGCATGGAAGAAACAGTGTGAAGAATTGTTAAATCTCATATTTCAATGTGAAGATTCAGAGCCTTTCCGTCAGCCGGTAGATCTCCTTGAATATCCAGACTACAGAGACATCATTGACACTCCAATGGATTTTGCTACCGTTAGAGAAACTTTAGAGGCTGGGAATTATGAGTCACCAATGGAGTTATGTAAAGATGTCAGACTTATTTTCAGTAATTCCAAAGCATATACACCAAGCAAAAGATCAAGGATTTACAGCATGAGTTTGCGCTTGTCTGCATTCTTTGAAGAACACATTAGTTCAGTTTTATCAGATTATAAATCTGCTCTTCGTTTTCATAAAAGAAATACCATAACCAAAAGGAGGAAGAAAAGAAACAGAAGCAGCTCTGTTTCCAGTAGTGCTGCATCAAGCCCTGAAAGGAAAAAAAGGATCTTAAAACCCCAGCTAAAATCAGAAAGCTCTACCTCTGCATTCTCTACACCTACACGATCAATACCGCCAAGACACAATGCTGCTCAGATAAACGGTAAAACAGAATCTAGTTCTGTGGTTCGAACCAGAAGCAACCGAGTGGTTGTAGATCCAGTTGTCACTGAGCAACCATCTACTTCTTCAGCTGCAAAGACTTTTATTACAAAAGCTAATGCATCTGCAATACCAGGGAAAACAATACTAGAGAATTCTGTGAAACATTCCAAAGCTTTGAATACTCTTTCCAGTCCTGGTCAATCCAGTTTTAGTCATGGCACTAGGAATAATTCTGCAAAAGAAAACATGGAAAAGGAAAAGCCAGTCAAACGTAAAATGAAGTCATCTGTACTCCCAAAGGCGTCCACTCTTTCAAAGTCATCAGCTGTCATTGAGCAAGGAGATTGTAAGAACAACGCTCTTGTACCAGGAACCATTCAAGTAAATGGCCATGGAGGACAGCCATCAAAACTTGTGAAGAGGGGACCTGGAAGGAAACCTAAAGTAGAAGTTAATACCAATAGTGGTGAAATTATACACAAGAAAAGGGGTAGAAAGCCCAAAAAGCTACAGTATGCAAAGCCAGAAGATTTAGAGCAAAATAATGTGCATCCCATCAGAGATGAAGTACTTCCTTCTTCAACATGCAATTTTCTTTCTGAAACTAATAATGTAAAGGAAGATTTGTTACAGAAAAAGAATCGTGGAGGTAGGAAGCCCAAAAGGAAGATGAAGACACAAAAATTAGATGCAGATCTCCTAGTCCCTGCAAGTGTCAAAGTGTTAAGGAGAAGTAACCGAAAAAAGATAGATGATCCTATAGATGAGGAAGAAGAGTTTGAAGAACTCAAAGGCTCTGAACCCCACATGAGAACTAGAAATCAAGGTCGAAGGACAGCTTTCTATAATGAGGATGACTCTGAAGAGGAGCAAAGGCAGCTGTTGTTCGAAGACACCTCTTTAACTTTTGGAACTTCTAGTAGAGGACGAGTCCGAAAGTTGACTGAAAAAGCAAAAGCTAATTTAATTGGTTGGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNF
EDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKL
DITSHNEDYTVVEQYERSEGRHSTGGMDELYKGSMSCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLPRRTDWTGKEHPRTYQNLVKY
YRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGRQSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERLVP
TAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGH
SASITSLQFSPLCSGSKRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGMFLATGSTDHIIRVYFFGSGQPEKISELEFHTD
KVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWKSILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLIHVLMGHE
DEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNMIEGQGHGAVFDCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLI
RDANNFVLDEQTQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQANQEISPLDSMIQRLQQEQDLRRSGEAGISN
TSRLSRGSISSTSEVHSPPNVGLRRSGQIEGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWRTAKGEEEIKTYRSEEKRKHLTVPKEN
KIPTVSKNHAHEHFLDLGESKKQQTNQHNYRTRSALEETPRPSEEIENGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWTADAGINLQPP
KKVPKNKTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKKKKPKERKQKRLAVGELTENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYY
FRQGHEAYVEMARKNKIYSINPKKQPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDTGKLTGGSFTMKYHDMPDVIDFLVLRQQFDDAKYRRW
NIGDRFRSVIDDAWWFGTIESQEPLQPEYPDSLFQCYNVCWDNGDTEKMSPWDMELIPNNAVFPEELGTSVPLTDGECRSLIYKPLDGEWGTNPRDEECE
RIVAGINQLMTLDIASAFVAPVDLQAYPMYCTVVAYPTDLSTIKQRLENRFYRRVSSLMWEVRYIEHNTRTFNEPGSPIVKSAKFVTDLLLHFIKDQTCY
NIIPLYNSMKKKVLSDSEDEEKDADVPGTSTRKRKDHQPRRRLRNRAQSYDIQAWKKQCEELLNLIFQCEDSEPFRQPVDLLEYPDYRDIIDTPMDFATV
RETLEAGNYESPMELCKDVRLIFSNSKAYTPSKRSRIYSMSLRLSAFFEEHISSVLSDYKSALRFHKRNTITKRRKKRNRSSSVSSSAASSPERKKRILK
PQLKSESSTSAFSTPTRSIPPRHNAAQINGKTESSSVVRTRSNRVVVDPVVTEQPSTSSAAKTFITKANASAIPGKTILENSVKHSKALNTLSSPGQSSF
SHGTRNNSAKENMEKEKPVKRKMKSSVLPKASTLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKL
QYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLDADLLVPASVKVLRRSNRKKIDDPIDEEEEFEELKGSEPHM
RTRNQGRRTAFYNEDDSEEEQRQLLFEDTSLTFGTSSRGRVRKLTEKAKANLIGW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63931
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone contains silent changes at:; Thr944 ACA > ACG; Leu1410 CTG > TTG; Ala1412 GCT > GCA; ; plus listed SNPs V663G and L1093P
• Low Temp: No