cDNA Clone Mitofusin 1

• Synonyms: hfzo1; hfzo2; MFN1
• Plasmid: pET15b 6His 3C Mitofusin 1 G537-S632/SG linker subsitution
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C Mitofusin 1 G537-S632/SG linker subsitution
• Genbank: NM_033540.3
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCTGGCTAAGAAGGCGATTACTGCAATCTTTGACCAGTTACTGGAGTTTGTTACTGAAGGATCACATTTTGTTGAAGCAACATATAAGAATCCGGAACTTGATCGAATAGCCACTGAAGATGATCTGGTAGAAATGCAAGGATATAAAGACAAGCTTTCCATCATTGGTGAGGTGCTATCTCGGAGACACATGAAGGTGGCATTTTTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAATGCAATGTTGTGGGATAAAGTTCTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCTAAGTGTTGAAGGAACTGATGGAGATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAAAAAGAGTGTGAAGACAGTTAATCAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAAAGCTGGCTGTCTTGTACGTGTGTTTTGGCCAAAAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTGTTAGTAGACAGTCCAGGCACAGATGTCACTACAGAGCTGGATAGCTGGATTGATAAGTTTTGCCTAGATGCTGATGTCTTTGTTTTGGTCGCAAACTCTGAATCAACACTAATGAATACGGAAAAACACTTTTTTCACAAGGTGAATGAGCGGCTTTCCAAGCCTAATATTTTCATTCTCAATAATCGTTGGGATGCCTCTGCATCAGAGCCAGAATATATGGAAGACGTACGCAGACAGCACATGGAAAGATGCCTGCATTTCTTGGTGGAGGAGCTCAAAGTTGTAAATGCTTTAGAAGCACAGAATCGTATCTTCTTTGTTTCAGCAAAGGAAGTTCTTAGTGCTAGAAAGCAAAAAGCACAGGGGATGCCAGAAAGTGGTGTGGCACTTGCTGAAGGATTTCATGCAAGATTACAGGAATTTCAGAATTTTGAACAAATCTTTGAGGAGTGTATCTCGCAGTCAGCAGTGAAAACAAAGTTCGAACAGCACACTATCAGAGCTAAACAGATACTAGCTACTGTGAAAAACATAATGGATTCAGTAAACCTGGCAGCTGAAGATAAAAGGCATTATTCAGTGGAAGAGAGGGAAGACCAAATTGATAGACTGGACTTTATTCGAAACCAGATGAACCTTTTAACACTGGATGTTAAGAAAAAAATCAAGGAGGTTACCGAGGAGGTGGCAAACAAAGTTTCATGTGCAATGACAGATGAAATTTGTCGACTGTCTGTTTTGGTTGATGAATTTTGTTCAGAGTTTCATCCTAATCCAGATGTATTAAAAATATATAAAAGTGAATTAAATAAGCACATAGAGGATGGTATGGGAAGAAATTTGGCTGATCGATGCACCGATGAAGTAAACGCCTTAGTGCTTCAGACCCAGCAAGAAATTATTGAAAATTTGAAGCCATTACTTCCAGCTGGTATACAGGATAAACTACATACACTGATCCCTTGCAAGAAATTTGATCTCAGTTATAATCTAAATTACCACAAGTTATGTTCAGATTTTCAAGAGGATATTGTATTTCGTTTTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTTTTGtcaggtagtggatcgggcgggTGGACCACCCATGCCAAGGAGCGAGCCTTTAAACAGCAGTTTGTAAACTATGCAACTGAAAAACTGAGGATGATTGTTAGCTCCACGAGTGCAAACTGCAGTCACCAAGTAAAACAACAAATAGCTACCACTTTTGCTCGCCTGTGCCAACAAGTTGATATTACTCAAAAACAGCTGGAAGAAGAAATTGCTAGATTACCCAAAGAAATAGATCAGTTGGAGAAAATACAAAACAATTCAAAGCTCTTAAGAAATAAAGCTGTTCAACTTGAAAATGAGCTGGAGAATTTTACTAAGCAGTTTCTACCTTCAAGCAATGAAGAATCCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMK
VAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLV
DSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQ
NRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQI
DRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEII
ENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLSGSGSGGWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCS
HQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6His 3C Mitofusin 1 G537-S632/SG linker subsitution350.76 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67040
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No