cDNA Clone diubiquitin

• Plasmid: pET24a cod op Ubiquitin T66V L67N-Ubiquitin
• Parent Plasmid: pET24a
• Expressed: Ubiquitin T66V L67N-ubiquitin
• Genbank: P62987.2 GI:302393718
• Tag: untagged
• Species: Human

Sequence of Insert

catATGCAGATCTTTGTTAAAACCCTGACCGGTAAAACCATTACACTGGAAGTTGAACCGAGCGATACCATTGAAAATGTGAAAGCCAAAATCCAGGACAAAGAAGGTATTCCGCCTGATCAGCAGCGTCTGATTTTTGCAGGTAAACAGCTGGAAGATGGTCGTACCCTGAGCGATTATAACATTCAGAAAGAAAGCgtgaacCATCTGGTTCTGCGTCTGCGTGGTGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGAgcggccgc

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESVNHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET24a cod op Ubiquitin T66V L67N-Ubiquitin221.63 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67053
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: First copy of Ubiquitin is codon-optimised.
• Low Temp: No