cDNA Clone IRAK3

• Synonyms: Interleukin-1 receptor-associated kinase 3, ASRT5, IRAKM, IL-1 receptor-associated kinase M
• Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 WT
• Parent Plasmid: pNL3.2
• Expressed: FLAG-IRAK3
• Genbank: NM_007199.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGAL
GWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQV
LQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLI
PEHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTY
AVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCL
IYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSV
ICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLW
YMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRE
LMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEV
LNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNL
LPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSC
EESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDE
YEQYKKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 WT 182.65 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65157
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at Gly40 (c120t) and a SNP at I147V.; Parental 65115 Dual NanoLuc reporter; The co-expressed NlucP reporter consists of NanoLuc® luciferase with a C-terminal fusion to PEST (see 65115 Vector data sheet for more info)
• Low Temp: No