cDNA Clone DNAJC13

• Synonyms: RME8; PARK21
• Plasmid: pcDNA5D FRT/TO HA DNAJC13
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA DNAJC13
• Genbank: NM_001329126.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGAACATAATTAGGGAAAATAAGGATCTGGCATGTTTCTACACAACAAAACATTCATGGAGGGGGAAGTATAAGCGTGTCTTTTCAGTTGGAACTCATGCGATTACTACATATAATCCCAATACCTTAGAAGTAACAAATCAGTGGCCTTATGGAGACATTTGCAGCATCAGCCCTGTTGGAAAAGGACAAGGAACGGAGTTCAACCTCACATTTCGTAAAGGCAGTGGAAAAAAGTCAGAAACTTTAAAATTTTCTACAGAGCACAGAACAGAACTTCTTACAGAAGCATTGAGATTTAGAACTGATTTTTCAGAGGGAAAAATCACAGGAAGGAGATACAACTGCTATAAGCATCACTGGAGTGACTCAAGAAAACCTGTAATTTTGGAAGTAACTCCAGGAGGCTTTGACCAAATTAATCCTGCAACCAACAGAGTACTCTGTTCCTATGACTATAGAAATATTGAAGGATTTGTAGATCTCTCAGATTATCAAGGAGGATTTTGTATACTTTATGGAGGATTTAGTAGATTGCATTTATTTGCGTCAGAGCAAAGAGAAGAGATTATTAAAAGTGCAATAGACCATGCTGGTAACTACATAGGTATTTCATTGCGGATCAGGAAAGAGCCTTTAGAATTTGAGCAATATTTGAATCTTCGCTTTGGAAAATACAGCACTGATGAATCCATCACATCTTTAGCAGAGTTTGTAGTCCAAAAAATATCACCTAGACATTCGGAGCCTGTTAAAAGAGTTCTAGCACTTACAGAAACATGTTTAGTAGAACGTGATCCGGCAACCTATAATATTGCAACATTGAAGCCTTTAGGAGAAGTATTTGCGTTGGTCTGTGACTCAGAAAATCCACAACTTTTTACCATTGAATTTATAAAAGGGCAAGTACGGAAATATTCTTCAACAGAGAGAGATTCCTTATTAGCAAGTTTGCTGGATGGAGTAAGAGCCTCTGGTAATAGAGATGTTTGTGTAAAAATGACACCAACCCATAAAGGTCAGCGATGGGGGTTACTCAGCATGCCTGTTGATGAGGAAGTAGAGAGCCTTCACCTCAGGTTCTTAGCTACGCCTCCAAATGGCAACTTTGCAGATGCTGTATTCAGGTTCAATGCTAATATTTCATACAGTGGAGTCCTACATGCAGTAACACAAGATGGTCTCTTCTCAGAAAACAAAGAAAAACTGATCAATAATGCCATAACAGCATTACTGTCCCAAGAAGGGGATGTCGTTGCTTCAAATGCGGAACTTGAGAGTCAGTTCCAGGCTGTGAGGAGGCTTGTGGCATCCAAAGCTGGTTTCCTGGCTTTCACTCAGCTTCCAAAGTTTCGCGAGCGTCTAGGGGTGAAGGTAGTAAAAGCACTCAAAAGAAGCAACAACGGAATAATCCATGCAGCAGTTGAGATGCTTTGTGCCCTTATGTGTCCCATGCATGATGACTATGACTTAAGACAAGAACAGTTGAACAAAGCTTCTCTTCTCTCGTCAAAGAAGTTTCTGGAAAACTTACTGGAGAAATTTAATTCCCATGTGGATCATGGGACTGGTGCCCTAGTTATTAGTTCGCTCTTGGACTTCCTTACCTTTGCCCTCTGTGCTCCATATAGTGAGACAACTGAAGGGCAGCAGTTTGATATGCTCTTGGAGATGGTAGCATCCAATGGAAGAACCCTTTTTAAACTTTTTCAGCATCCTTCCATGGCAATAATAAAAGGAGCTGGGTTGGTTATGAAGGCAATAATAGAGGAAGGTGATAAAGAAATTGCTACAAAAATGCAGGAGCTTGCCCTAAGTGAAGGTGCCTTACCTCGACACTTGCATACTGCGATGTTTACAATAAGCTCAGATCAAAGGATGCTTACAAATAGACAGCTAAGTAGACATTTAGTGGGACTCTGGACAGCTGATAATGCAACTGCAACAAACTTGTTGAAACGCATTTTGCCGCCAGGCTTGCTGGCATACTTGGAAAGCTCAGATCTCGTACCTGAGAAGGATGCTGATCGGATGCATGTTAGAGACAATGTGAAAATAGCAATGGATCAGTATGGAAAATTTAATAAAGTTCCAGAGTGGCAAAGACTAGCTGGAAAAGCTGCTAAAGAAGTTGAAAAATTTGCCAAAGAAAAAGTGGATCTTGTATTGATGCACTGGAGGGATAGGATGGGCATTGCTCAAAAAGAGAATATAAATCAGAAGCCAGTGGTTCTTCGAAAGAGAAGACAAAGAATAAAAATAGAAGCAAATTGGGATCTCTTCTATTATAGGTTTGGTCAAGACCATGCCAGGTCAAACCTTATTTGGAATTTCAAAACACGAGAAGAACTGAAAGATACTCTTGAATCTGAAATGAGAGCATTTAATATTGACAGAGAACTTGGAAGTGCAAATGTGATCTCCTGGAACCACCATGAGTTTGAGGTTAAATATGAGTGCCTGGCAGAGGAAATTAAAATAGGAGACTATTACCTGAGATTACTATTGGAGGAAGATGAGAATGAAGAAAGTGGATCAATTAAGAGATCGTATGAATTTTTCAATGAGCTTTATCATCGCTTCTTGCTCACCCCAAAAGTAAACATGAAGTGTTTATGTTTACAAGCCCTTGCTATTGTTTATGGCAGATGTCACGAAGAAATAGGACCTTTTACAGATACCAGATATATCATTGGAATGTTAGAGAGGTGCACAGATAAACTTGAACGAGATAGGTTGATTCTCTTCCTTAACAAGTTGATCCTTAATAAGAAAAATGTTAAGGATCTCATGGATTCAAATGGAATAAGAATCCTTGTGGACTTGCTTACCCTTGCACATCTCCATGTAAGCCGAGCTACAGTACCACTGCAAAGCAATGTAATTGAAGCTGCTCCAGATATGAAAAGAGAGAGTGAAAAGGAATGGTATTTTGGCAACGCAGACAAAGAAAGGAGTGGCCCGTATGGATTTCATGAGATGCAAGAATTGTGGACCAAAGGAATGTTAAATGCAAAAACCAGATGCTGGGCTCAAGGCATGGATGGATGGCGACCACTTCAGTCCATACCCCAGCTTAAGTGGTGTCTCTTAGCCAGTGGACAGGCTGTCCTGAATGAAACTGACCTTGCTACCCTTATATTGAACATGTTGATCACAATGTGTGGATATTTTCCAAGCAGGGATCAAGACAATGCCATCATTCGGCCTCTACCCAAAGTGAAAAGACTGCTGTCAGATAGCACTTGCCTTCCCCATATTATTCAGCTACTGCTGACCTTTGACCCTATCCTTGTTGAGAAGGTTGCTATTTTGTTATACCATATCATGCAAGATAACCCACAGTTACCCCGCCTTTATCTGAGTGGAGTATTTTTCTTTATCATGATGTACACAGGTTCCAATGTGCTTCCTGTTGCTCGATTTTTGAAATACACACATACCAAACAGGCTTTCAAGTCAGAAGAGACAAAAGGACAAGATATTTTTCAGAGAAGTATACTTGGGCACATTCTACCTGAAGCAATGGTTTGTTACTTAGAAAATTATGAACCTGAAAAGTTTTCTGAGATTTTTCTAGGAGAATTTGATACTCCAGAAGCAATCTGGAGCAGTGAAATGAGGCGCCTGATGATAGAGAAGATTGCTGCCCATCTCGCGGATTTCACACCTCGTCTTCAGAGTAACACAAGAGCACTTTATCAGTATTGCCCCATTCCTATAATCAACTATCCACAACTCGAAAATGAACTATTTTGTAATATTTATTACCTCAAACAACTGTGTGATACACTCCGGTTTCCAGATTGGCCAATTAAAGACCCGGTTAAGCTTCTAAAAGATACCCTTGATGCCTGGAAGAAAGAAGTAGAAAAGAAGCCACCTATGATGTCAATAGATGATGCTTATGAAGTGCTTAATCTGCCTCAAGGACAGGGACCGCATGATGAGAGCAAGATTAGGAAAGCTTACTTCAGACTTGCACAAAAGTACCACCCTGATAAGAATCCAGAAGGGAGGGACATGTTTGAAAAAGTAAATAAAGCATATGAATTTTTATGTACCAAATCAGCAAAAATAGTGGATGGGCCAGATCCAGAGAATATAATTTTAATTCTAAAAACACAGAGCATCCTCTTCAACCGTCATAAAGAAGATTTACAGCCTTATAAATATGCAGGATACCCCATGCTTATTCGGACTATAACAATGGAAACTTCAGATGACCTCCTTTTCTCAAAAGAATCACCATTGTTGCCTGCGGCTACAGAGCTAGCTTTCCATACTGTCAACTGTTCAGCCCTCAATGCTGAAGAGCTCAGAAGAGAGAATGGACTAGAGGTGTTACAAGAGGCATTTAGTCGCTGTGTGGCTGTCTTGACTCGTTCTAGTAAACCAAGTGACATGTCAGTACAGGTGTGTGGATACATAAGTAAATGCTACAGTGTGGCTGCTCAGTTTGAGGAATGCCGAGAGAAGATCACGGAAATGCCTAGCATCATCAAGGATCTCTGTCGGGTACTATATTTTGGCAAGAGTATTCCCCGCGTAGCTGCTCTTGGGGTAGAATGTGTCAGTTCTTTTGCTGTGGATTTCTGGCTACAGACACACCTATTTCAGGCTGGAATTTTGTGGTATCTCCTTGGTTTTCTGTTTAATTATGACTACACACTAGAAGAGAGTGGCATTCAGAAAAGTGAAGAAACAAACCAGCAGGAGGTAGCAAACAGCCTTGCCAAACTGAGTGTCCATGCTCTGAGTCGCCTTGGAGGGTATTTGGCTGAAGAACAAGCAACTCCAGAAAATCCAACCATAAGGAAAAGCTTAGCTGGCATGCTGACACCCTATGTTGCTAGAAAACTTGCTGTGGCTAGTGTGACTGAGATTTTGAAGATGCTTAACAGCAACACAGAAAGTCCATATTTGATATGGAACAATTCTACAAGAGCAGAATTACTTGAATTTCTTGAATCCCAACAAGAAAACATGATTAAAAAAGGTGATTGTGACAAAACTTATGGATCAGAATTTGTCTACAGTGATCATGCCAAAGAACTTATTGTAGGGGAGATTTTTGTTAGGGTGTATAATGAAGTTCCTACTTTCCAACTGGAGGTTCCAAAAGCATTTGCTGCAAGTCTCTTGGATTATATAGGCTCGCAGGCCCAATACTTGCACACATTCATGGCCATCACACACGCGGCAAAAGTGGAGTCAGAGCAACATGGAGATCGCTTACCGAGAGTAGAAATGGCTTTGGAGGCTCTGAGAAATGTCATAAAATACAATCCAGGTTCTGAGAGTGAATGCATTGGGCACTTTAAGTTGATATTTTCTCTTCTCCGAGTTCATGGAGCTGGTCAAGTGCAGCAGTTGGCTTTAGAGGTTGTGAATATAGTGACATCTAACCAAGACTGTGTCAACAATATTGCTGAATCAATGGTTTTGTCCAGTTTATTGGCTCTTCTACATTCATTGCCATCAAGTCGTCAGCTTGTTCTGGAAACTCTTTATGCTTTGACATCGAGTACAAAAATAATCAAAGAAGCAATGGCAAAGGGTGCTTTGATCTATTTACTGGATATGTTCTGCAATTCAACACATCCACAGGTTCGAGCCCAAACAGCAGAACTTTTTGCCAAAATGACAGCAGATAAACTGATAGGTCCAAAGGTTCGAATTACGTTAATGAAATTTCTACCAAGCGTTTTCATGGATGCTATGAGAGACAATCCTGAAGCTGCTGTACATATTTTTGAAGGAACTCATGAAAATCCTGAGTTAATTTGGAATGATAATTCCAGAGATAAAGTGTCCACAACAGTTAGGGAAATGATGCTAGAGCACTTTAAAAATCAGCAGGACAACCCTGAGGCAAACTGGAAGTTGCCTGAAGATTTTGCTGTGGTGTTTGGAGAAGCAGAGGGTGAACTTGCTGTTGGAGGAGTCTTCTTGAGGATCTTTATTGCACAACCAGCCTGGGTTCTAAGAAAGCCTAGAGAATTTCTTATTGCCCTGTTAGAAAAATTAACTGAGCTCCTAGAGAAGAACAATCCTCATGGAGAAACTCTGGAAACCTTGACAATGGCAACAGTGTGTCTCTTCAGCGCACAACCTCAGCTGGCAGATCAGGTCCCGCCATTGGGCCATCTTCCCAAAGTTATCCAGGCAATGAATCATAGGAACAATGCCATTCCTAAGAGTGCCATTCGGGTTATCCATGCCTTGTCTGAAAATGAGCTGTGTGTTCGAGCCATGGCATCTTTAGAGACCATTGGCCCACTGATGAATGGAATGAAAAAGCGAGCAGATACTGTTGGTCTAGCCTGTGAAGCAATTAATCGAATGTTTCAGAAGGAGCAGAGTGAATTAGTAGCACAAGCCCTGAAAGCAGATTTGGTTCCATACCTCTTAAAATTACTCGAAGGCATTGGCCTTGAAAACCTGGACAGCCCAGCAGCCACTAAGGCTCAGATTGTTAAAGCTCTCAAGGCAATGACTCGAAGTTTGCAGTATGGAGAACAGGTGAATGAAATCCTGTGCCGTTCTTCAGTCTGGAGTGCCTTCAAAGATCAGAAACATGATTTGTTCATTTCTGAGTCACAAACAGCAGGATACCTCACAGGACCTGGAGTTGCTGGCTACCTTACCGCAGGTACATCTACATCAGTCATGTCTAACCTGCCACCTCCTGTAGACCATGAGGCAGGCGACCTTGGCTATCAGACTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMNIIRENKDLACFYTTKHSWRGKYKRVFSVGTHAITTYNPNTLEVTNQWPYGDICSISPVGKGQGTEFNLTFRKGSGKKSETLKFSTEHRTELLTEALRFRTDFSEGKITGRRYNCYKHHWSDSRKPVILEVTPGGFDQINPATNRVLCSYDYRNIEGFVDLSDYQGGFCILYGGFSRLHLFASEQREEIIKSAIDHAGNYIGISLRIRKEPLEFEQYLNLRFGKYSTDESITSLAEFVVQKISPRHSEPVKRVLALTETCLVERDPATYNIATLKPLGEVFALVCDSENPQLFTIEFIKGQVRKYSSTERDSLLASLLDGVRASGNRDVCVKMTPTHKGQRWGLLSMPVDEEVESLHLRFLATPPNGNFADAVFRFNANISYSGVLHAVTQDGLFSENKEKLINNAITALLSQEGDVVASNAELESQFQAVRRLVASKAGFLAFTQLPKFRERLGVKVVKALKRSNNGIIHAAVEMLCALMCPMHDDYDLRQEQLNKASLLSSKKFLENLLEKFNSHVDHGTGALVISSLLDFLTFALCAPYSETTEGQQFDMLLEMVASNGRTLFKLFQHPSMAIIKGAGLVMKAIIEEGDKEIATKMQELALSEGALPRHLHTAMFTISSDQRMLTNRQLSRHLVGLWTADNATATNLLKRILPPGLLAYLESSDLVPEKDADRMHVRDNVKIAMDQYGKFNKVPEWQRLAGKAAKEVEKFAKEKVDLVLMHWRDRMGIAQKENINQKPVVLRKRRQRIKIEANWDLFYYRFGQDHARSNLIWNFKTREELKDTLESEMRAFNIDRELGSANVISWNHHEFEVKYECLAEEIKIGDYYLRLLLEEDENEESGSIKRSYEFFNELYHRFLLTPKVNMKCLCLQALAIVYGRCHEEIGPFTDTRYIIGMLERCTDKLERDRLILFLNKLILNKKNVKDLMDSNGIRILVDLLTLAHLHVSRATVPLQSNVIEAAPDMKRESEKEWYFGNADKERSGPYGFHEMQELWTKGMLNAKTRCWAQGMDGWRPLQSIPQLKWCLLASGQAVLNETDLATLILNMLITMCGYFPSRDQDNAIIRPLPKVKRLLSDSTCLPHIIQLLLTFDPILVEKVAILLYHIMQDNPQLPRLYLSGVFFFIMMYTGSNVLPVARFLKYTHTKQAFKSEETKGQDIFQRSILGHILPEAMVCYLENYEPEKFSEIFLGEFDTPEAIWSSEMRRLMIEKIAAHLADFTPRLQSNTRALYQYCPIPIINYPQLENELFCNIYYLKQLCDTLRFPDWPIKDPVKLLKDTLDAWKKEVEKKPPMMSIDDAYEVLNLPQGQGPHDESKIRKAYFRLAQKYHPDKNPEGRDMFEKVNKAYEFLCTKSAKIVDGPDPENIILILKTQSILFNRHKEDLQPYKYAGYPMLIRTITMETSDDLLFSKESPLLPAATELAFHTVNCSALNAEELRRENGLEVLQEAFSRCVAVLTRSSKPSDMSVQVCGYISKCYSVAAQFEECREKITEMPSIIKDLCRVLYFGKSIPRVAALGVECVSSFAVDFWLQTHLFQAGILWYLLGFLFNYDYTLEESGIQKSEETNQQEVANSLAKLSVHALSRLGGYLAEEQATPENPTIRKSLAGMLTPYVARKLAVASVTEILKMLNSNTESPYLIWNNSTRAELLEFLESQQENMIKKGDCDKTYGSEFVYSDHAKELIVGEIFVRVYNEVPTFQLEVPKAFAASLLDYIGSQAQYLHTFMAITHAAKVESEQHGDRLPRVEMALEALRNVIKYNPGSESECIGHFKLIFSLLRVHGAGQVQQLALEVVNIVTSNQDCVNNIAESMVLSSLLALLHSLPSSRQLVLETLYALTSSTKIIKEAMAKGALIYLLDMFCNSTHPQVRAQTAELFAKMTADKLIGPKVRITLMKFLPSVFMDAMRDNPEAAVHIFEGTHENPELIWNDNSRDKVSTTVREMMLEHFKNQQDNPEANWKLPEDFAVVFGEAEGELAVGGVFLRIFIAQPAWVLRKPREFLIALLEKLTELLEKNNPHGETLETLTMATVCLFSAQPQLADQVPPLGHLPKVIQAMNHRNNAIPKSAIRVIHALSENELCVRAMASLETIGPLMNGMKKRADTVGLACEAINRMFQKEQSELVAQALKADLVPYLLKLLEGIGLENLDSPAATKAQIVKALKAMTRSLQYGEQVNEILCRSSVWSAFKDQKHDLFISESQTAGYLTGPGVAGYLTAGTSTSVMSNLPPPVDHEAGDLGYQT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU68127
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation F215; 2 naturally occuring SNP D476E A1463S
• Low Temp: No