cDNA Clone CTF18

• Plasmid: pGEX6P1 CTF18 1-222
• Parent Plasmid: pGEX6P1
• Expressed: GST-CTF18 N Terminal (1-222)
• Genbank: NM_022092.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGGACTACGAGCAGGAGCTGTGCGGCGTCGAGGATGATTTCCACAACCAGTTCGCGGCCGAGCTGGAGGTGCTGGCAGAGCTGGAAGGGGCGTCGACTCCGTCGCCCTCCGGGGTCCCCCTGTTCACCGCGGGCCGACCCCCGCGGACGTTCGAGGAGGCCCTTGCCAGAGGGGACGCGGCCTCCAGTCCCGCCCCAGCCGCATCTGTGGGCAGCAGCCAGGGCGGCGCCAGGAAGAGGCCGGTGGACGCCGACCTGCAGCCGGCCGGGTCCCTGCCCCACGCCCCCAGGATCAAACGGCCTAGGCTGCAGGTGGTCAAGAGGCTGAACTTCAGGTCGGAGGAGATGGAGGAGCCGCCCCCTCCCGACTCCTCGCCGACGGACATCACCCCGCCGCCGAGCCCTGAGGACCTCGCAGAGCTTTGGGGCCACGGAGTCTCAGAAGCTGCTGCCGACGTGGGTCTCACACGGGCCTCACCAGCTGCCCGCAATCCCGTCCTGAGGCGGCCCCCCATCTTGGAGGACTACGTCCACGTGACATCCACGGAGGGCGTCCGGGCTTATCTGGTGCTGCGTGCTGACCCCATGGCCCCGGGGGTGCAGGGCTCTCTCCTCCACGTCCCATGGCGAGGCGGTGGCCAGCTGGACCTGCTGGGTGTGTCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEDYEQELCGVEDDFHNQFAAELEVLAELEGASTPSPSGVPLFTAGRPPRTFEEALARGDAASSPAPAASVGSSQGGARKRPVDADLQPAGSLPHAPRIKRPRLQVVKRLNFRSEEMEEPPPPDSSPTDITPPPSPEDLAELWGHGVSEAAADVGLTRASPAARNPVLRRPPILEDYVHVTSTEGVRAYLVLRADPMAPGVQGSLLHVPWRGGGQLDLLGVS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU66213
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent A339G, SNP Q75P; ; Notes: Please can you make a construct to introduce 6His at the N-terminus of Homo sapiens CTF18 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.
• Low Temp: No