Synonyms
NHLRC1
Plasmid
pET15b 6xHIS TEV HALO 3C Malin Ala113-Gly395
Parent Plasmid
pET15b 6xHIS TEV HALO 3C
Expressed
6His-Halo-Malin[113-395]
Genbank
NM_198586
Tag
6His
HALO
Species
Human
Sequence of Insert
View Sequence
ggatccGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGT
CAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGG
TGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCC
TGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCG
GCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
ATTGGAGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAA
TGGGATTGTGGTAACTGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACG
TCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCAT
CTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGC
GGTCCTGGAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGG
TGAAAGTGTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTT
GGGCTGAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTT
TGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCC
TTTGCTTAGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACT
CATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCT
TCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACT
GGGGGTGAgcggccgc
CAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGG
TGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCC
TGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCG
GCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
ATTGGAGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAA
TGGGATTGTGGTAACTGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACG
TCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCAT
CTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGC
GGTCCTGGAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGG
TGAAAGTGTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTT
GGGCTGAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTT
TGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCC
TTTGCTTAGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACT
CATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCT
TCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACT
GGGGGTGAgcggccgc
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSALTCHHTFGGWGT
LVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIR
YPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTP
QNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGA
IAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAV
TFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVALTFTKEN
SLLVLDTASHSIKVYKVDWG*
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSALTCHHTFGGWGT
LVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIR
YPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTP
QNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGA
IAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAV
TFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVALTFTKEN
SLLVLDTASHSIKVYKVDWG*
Vector Type
Bacterial
Antibiotic
Amp
Full Snapgene Map
pET15b 6xHIS TEV HALO 3C Malin Ala113-Gly39568.8 KB
Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU65311
Unit Source
Location
PPU
MTA
Notes
Use TEV to cleave off 6xHIS. Precission may be used downstream to remove the HALO tag if necessary.
Parental 44459
Parental 44459
Low Temp
No
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.