cDNA Clone IRAK3

• Plasmid: pRetrox Tight Puro IRAK3 E480A
• Parent Plasmid: pRetrox tight puro FLAG APEX2
• Expressed: hu IRAK3[E480A]
• Genbank: NP_009130.2
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACAC
GCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTC
TGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTT
TCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGG
TAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGA
CCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCT
ATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAG
TTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATG
TCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTG
CTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTT
CCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAG
TGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAA
AAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGT
TTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTA
CAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACA
CTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCA
CATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGC
ACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAAC
ATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGC
ACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCA
GCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAG
GGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAAT
GGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCC
AGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCA
TGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTC
TGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGT
TAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCC
AGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTG
TCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGcAGATG
ATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATA
GACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTAT
GTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTT
GCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATA
GTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTC
AGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTT
CCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAAGCG
GCCGC

Amino Acid Sequence

MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGLAERLSS
SWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQVLQEMGHRRAIH
LITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIPEHNEKGVLLK
SSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKM
QCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLF
DRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSVICGSISSANIL
LDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGK
LSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCL
SFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASL
YFAEDPPTSLKSFRCPSPLFLENVPSIPVADDESQNNNLLPSDEGLRIDR
MTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVP
SQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65324
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No