cDNA Clone Malin

• Synonyms: NHLRC1
• Plasmid: pET28a-HALO-TEV-Malin Ala113-Gly395
• Parent Plasmid: pET28a-HALO-TEV
• Expressed: Halo-Malin[113-395]
• Genbank: NM_198586
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGT
CAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGG
TGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCC
TGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCG
GCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
ATTGGAGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAA
TGGGATTGTGGTAACTGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACG
TCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCAT
CTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGC
GGTCCTGGAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGG
TGAAAGTGTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTT
GGGCTGAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTT
TGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCC
TTTGCTTAGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACT
CATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCT
TCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACT
GGGGGTGAgcggccgc

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDS
GGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQ
IKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTER
LQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVFSSSMQLVGQ
VDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPEEFPVPK
PMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU65312
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 23222 (no 6His as Halo tag in vector cloned into Nco1 site, thereby deleting the 6His from pEt28a backbone)
• Low Temp: No