cDNA Clone Malin

• Synonyms: NHLRC1
• Plasmid: pET15b 6xHIS TEV HALO 3C Malin Ala113-Gly395 (delta F215-D233)
• Parent Plasmid: pET15b 6xHIS TEV HALO 3C
• Expressed: 6His-Halo-Malin[113-395] (delta F215-D233)
• Genbank: NM_198586
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGT
CAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGG
TGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCC
TGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCG
GCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
ATTGGAGGCCAAGCGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTT
CGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCATCTGTGCA
ATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTG
GAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGT
GTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCTGA
GCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTTTGATCAC
CAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTT
AGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACTCATGGTC
TTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCTTCTTGTG
CTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGGTG
Agcggccgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSALTCHHTFGGWGT
LVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIR
YPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQAEAGSLHLLDV
DFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRV
KVFSSSMQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAIL
CLGKPEEFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDW
G*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65325
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Use TEV to cleave off 6xHIS. Precission may be used downstream to remove the HALO tag if necessary. ; Parental 44459
• Low Temp: No