cDNA Clone NT5DC2

• Synonyms: -
• Plasmid: pCMV5D HA NT5DC2 D112A
• Parent Plasmid: pCMV5D HA
• Expressed: HA-NT5DC2 D112A
• Genbank: NM_001134231.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGGGTGCGGGGCTGCGGGCTGCAGCTCGGCGCTGGCTGCTGTGCGGAGGCCACGGCGGGCCGCGAGCCGCCTCGTCCTCGCCCTCCTGCCCTGGGTGCGGCCCCCCGGGTCCCGGCGCCCACTGCCCCGGCGTCCCGCGCTCCGCGCCCGCCCAGGCACCCACCAGCGGCGCCGACCTCAGCGCGCACCTATGGGCTCGCTACCAGGACATGCGGAGACTGGTGCACGACCTCCTGCCCCCCGAGGTCTGCAGTCTCCTGAACCCAGCAGCCATCTACGCCAACAACGAGATCAGCCTGCGTGACGTTGAGGTCTACGGCTTTGACTACGCCTACACCCTGGCCCAGTATGCAGACGCACTGCACCCCGAGATCTTCAGTACCGCCCGTGACATCCTGATCGAGCACTACAAGTACCCAGAAGGGATTCGGAAGTATGACTACAACCCCAGCTTTGCCATCCGTGGCCTCCACTATGACATTCAGAAGAGCCTTCTGATGAAGATTGACGCCTTCCACTACGTGCAGCTGGGGACAGCCTACAGGGGCCTCCAGCCTGTGCCAGACGAGGAGGTGATTGAGCTGTATGGGGGTACCCAGCACATCCCACTATACCAGATGAGTGGCTTCTATGGCAAGGGTCCCTCCATTAAGCAGTTCATGGACATCTTCTCGCTACCGGAGATGGCTCTGCTGTCCTGTGTGGTGGACTACTTTCTGGGCCACAGCCTGGAGTTTGACCAAGCACATCTCTACAAGGACGTGACGGACGCCATCCGAGACGTGCATGTGAAGGGCCTCATGTACCAGTGGATCGAGCAGGACATGGAGAAGTACATCCTGAGAGGGGATGAGACGTTTGCTGTCCTGAGCCGCCTGGTGGCCCATGGGAAACAGCTGTTCCTCATCACCAACAGTCCTTTCAGCTTCGTAGACAAGGGGATGCGGCACATGGTGGGTCCCGATTGGCGCCAGCTCTTCGATGTGGTCATTGTCCAGGCAGACAAGCCCAGCTTCTTCACTGACCGGCGCAAGCCTTTCAGAAAACTCGATGAGAAGGGCTCACTTCAGTGGGACCGGATCACCCGCTTGGAAAAGGGCAAGATCTATCGGCAGGGAAACCTGTTTGACTTCTTACGCTTGACGGAATGGCGTGGCCCCCGCGTGCTCTACTTCGGGGACCACCTCTATAGTGATCTGGCGGATCTCATGCTGCGGCACGGCTGGCGCACAGGCGCCATCATCCCCGAGCTGGAGCGTGAGATCCGCATCATCAACACGGAGCAGTACATGCACTCGCTGACGTGGCAGCAGGCGCTCACGGGGCTGCTGGAGCGCATGCAGACCTATCAGGACGCGGAGTCGAGGCAGGTGCTGGCTGCCTGGATGAAAGAGCGGCAGGAGCTGAGGTGCATCACCAAGGCCCTGTTCAATGCGCAGTTCGGCAGCATCTTCCGCACCTTCCACAACCCCACCTACTTCTCAAGGCGCCTCGTGCGCTTCTCTGACCTCTACATGGCCTCCCTCAGCTGCCTGCTCAACTACCGCGTGGACTTCACCTTCTACCCACGCCGTACGCCGCTGCAGCACGAGGCACCCCTCTGGATGGACCAGCTCTGCACCGGCTGCATGAAGACCCCCTTCCTTGGTGACATGGCCCACATCCGCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAGAGLRAAARRWLLCGGHGGPRAASSSPSCPGCGPPGPGAHCPGVPRSAPAQAPTSGADLSAHLWARYQDMRRLVHDLLPPEVCSLLNPAAIYANNEISLRDVEVYGFDYAYTLAQYADALHPEIFSTARDILIEHYKYPEGIRKYDYNPSFAIRGLHYDIQKSLLMKIDAFHYVQLGTAYRGLQPVPDEEVIELYGGTQHIPLYQMSGFYGKGPSIKQFMDIFSLPEMALLSCVVDYFLGHSLEFDQAHLYKDVTDAIRDVHVKGLMYQWIEQDMEKYILRGDETFAVLSRLVAHGKQLFLITNSPFSFVDKGMRHMVGPDWRQLFDVVIVQADKPSFFTDRRKPFRKLDEKGSLQWDRITRLEKGKIYRQGNLFDFLRLTEWRGPRVLYFGDHLYSDLADLMLRHGWRTGAIIPELEREIRIINTEQYMHSLTWQQALTGLLERMQTYQDAESRQVLAAWMKERQELRCITKALFNAQFGSIFRTFHNPTYFSRRLVRFSDLYMASLSCLLNYRVDFTFYPRRTPLQHEAPLWMDQLCTGCMKTPFLGDMAHIR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU66269
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutate D112A in DU68076, silent G24T, C27A
• Low Temp: No