cDNA Clone CHMP2A

• Synonyms: charged multivesicular body protein 2A, mVps2, 1500016L11Rik
• Plasmid: pBabe.puro-DU (mu)CHMP2A-C-GFP
• Parent Plasmid: pBabe.puro-DU C-GFP
• Expressed: CHMP2A-GFP
• Genbank: NM_001360730.1 → NP_001347659.1 Gene ID 68953
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCATGGACCTGTTGTTTGGGCGCCGGAAGACGCCAGAGGAACTACTTCGGCAAAACCAGAGGGCCCTGAACCGAGCCATGAGAGAACTGGACAGGGAACGACAGAAACTAGAAACCCAGGAAAAGAAAATCATTGCGGACATCAAAAAGATGGCAAAGCAAGGCCAGATGGATGCTGTTCGAATCATGGCAAAAGACCTGGTGCGTACCCGGAGATATGTACGCAAGTTTGTGTTGATGCGGGCCAACATCCAAGCTGTGTCCCTCAAGATACAGACTCTAAAATCCAACAACTCAATGGCACAAGCCATGAAGGGTGTAACTAAGGCCATGGGCACTATGAACAGACAGCTGAAATTACCCCAGATCCAGAAGATCATGATGGAGTTTGAACGGCAGGCAGAGATCATGGACATGAAGGAAGAAATGATGAATGACGCAATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATGCTGTTGTGTCCCAGGTCCTGGATGAGCTGGGACTGAGCCTCACAGATGAGCTGTCAAACCTTCCCTCCACTGGAGGCTCACTCAGTGTGGCAGCTGGTGGGAAGAAAGCAGAGGCCACAGCCTCAGCCCTGGCTGATGCTGATGCAGACCTGGAAGAGAGGCTCAAGAACCTTCGCAGGGACGCGGCCGC

Amino Acid Sequence

MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKM
AKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMA
QAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDA
MGDEEDEEESDAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEA
TASALADADADLEERLKNLRRDAAAMVSKGEELFTGVVPILVELDGDVNG
HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRY
PDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDG
SVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
TAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBabe.puro-DU (mu)CHMP2A-C-GFP53.18 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65492
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 40432. Isoform a; silent change at V106 (GTT > GTA) compared to NCBI ref; NM_001360730.1 is identical to NM_026885.3
• Low Temp: No