cDNA Clone TAK1

• Synonyms: CSCF, FMD2, MAP3K7, MEKK7, TGF1a
• Plasmid: pRetroX (Hygro)-TAK1 D175A (64616/64617 resistant)
• Parent Plasmid: pRetroX Hygro
• Expressed: TAK1
• Genbank: NM_003188.4
• Species: Human

Sequence of Insert

ggatccATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGC
aGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAaGTcGAgGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACAGTTCTAAAAATTTGTGcTTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAG
TACAGGCTCATTCATGGACATTGCTTCTACAAATACGAGTAACAAAAGTG
ACACTAATATGGAGCAAGTTCCTGCCACAAATGATACTATTAAGCGCTTA
GAATCAAAATTGTTGAAAAATCAGGCAAAGCAACAGAGTGAATCTGGACG
TTTAAGCTTGGGAGCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCCCCAA
CCTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGG
ATCGCCGCAACCACAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGA
CTTGACTGTAACTGGAACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCA
GTCCCAGTGTCAGAATGATTACTACCTCAGGACCAACCTCAGAAAAGCCA
ACTCGAAGTCATCCATGGACCCCTGATGATTCCACAGATACCAATGGATC
AGATAACTCCATCCCAATGGCTTATCTTACACTGGATCACCAACTACAGC
CTCTAGCACCGTGCCCAAACTCCAAAGAATCTATGGCAGTGTTTGAACAG
CATTGTAAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGCATT
GTTATTACAGAGAAAGCAAGAACTAGTTGCAGAACTGGACCAGGATGAAA
AGGACCAGCAAAATACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTA
GATGAAAACAAAAGCCTTTCTACTTACTACCAGCAATGCAAAAAACAACT
AGAGGTCATCAGAAGTCAGCAGCAGAAACGACAAGGCACTTCATGAgcgg
ccgc

Amino Acid Sequence

MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVV
CKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPV
CLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPK
ALIHRDLKPPNLLLVAGGTVLKICAFGTACDIQTHMTNNKGSAAWMAPEV
FEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPP
LIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQY
PCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLES
KLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIA
ATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTR
SHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHC
KMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDE
NKSLSTYYQQCKKQLEVIRSQQQKRQGTS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRetroX (Hygro)-TAK1 D175A (64616/64617 resistant)1.45 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65572
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 46490 pRetroX Hygro; clone harbours silent mutations to protect against guides DU64616 (Ala15 GCC>GCA) and DU64617 (Glu37 GAG>GAA, Val38 GTG>GTC, Glu39 GAA>GAG)
• Low Temp: No