cDNA Clone Malin

• Plasmid: pET-Duet1-6His-TEV-laforin, GST-3C-Malin
• Parent Plasmid: pGEX6P-1
• Expressed: GST-RV3C-Malin
• Genbank: Q6VVB1
• Tag: GST
• Species: Human

Sequence of Insert

CATatgtcccctatactaggttattggaaaattaagggccttgtgcaacc
cactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgt
atgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggt
ttggagtttcccaatcttccttattatattgatggtgatgttaaattaac
acagtctatggccatcatacgttatatagctgacaagcacaacatgttgg
gtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggtt
ttggatattagatacggtgtttcgagaattgcatatagtaaagactttga
aactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgt
tcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacc
catcctgacttcatgttgtatgacgctcttgatgttgttttatacatgga
cccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgta
ttgaagctatcccacaaattgataagtacttgaaatccagcaagtatata
gcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcc
tccaaaatcggatctggaagttctgttccaggggcccctgGGATCCATGG
CGGCCGAAGCCTCGGAGAGCGGGCCAGCGCTGCATGAGCTCATGCGCGAG
GCGGAGATCAGCCTGCTCGAGTGCAAGGTGTGCTTTGAGAAGTTTGGCCA
CCGGCAGCAGCGGCGCCCGCGCAACCTGTCCTGCGGCCACGTGGTCTGCC
TGGCCTGCGTGGCCGCCCTGGCGCACCCGCGCACTCTGGCCCTCGAGTGC
CCATTCTGCAGGCGAGCTTGCCGGGGCTGCGACACCAGCGACTGCCTGCC
GGTGCTGCACCTCATAGAGCTCCTGGGCTCAGCGCTTCGCCAGTCCCCGG
CCGCCCATCGCGCCGCCCCCAGCGCCCCCGGAGCCCTCACCTGCCACCAC
ACCTTCGGCGGCTGGGGGACCCTGGTCAACCCCACCGGACTGGCGCTTTG
TCCCAAGACGGGGCGTGTCGTGGTGGTGCACGACGGCAGGAGGCGTGTCA
AGATTTTTGACTCAGGGGGAGGATGCGCGCATCAGTTTGGAGAGAAGGGG
GACGCTGCCCAAGACATTAGGTACCCTGTGGATGTCACCATCACCAACGA
CTGCCATGTGGTTGTCACTGACGCCGGCGATCGCTCCATCAAAGTGTTTG
ATTTTTTTGGCCAGATCAAGCTTGTCATTGGAGGCCAATTCTCCTTACCT
TGGGGTGTGGAGACCACCCCTCAGAATGGGATTGTGGTAACTGATGCGGA
GGCAGGGTCCCTGCACCTCCTGGACGTCGACTTCGCGGAAGGGGTCCTTC
GGAGAACTGAAAGGTTGCAAGCTCATCTGTGCAATCCCCGAGGGGTGGCA
GTGTCTTGGCTCACCGGGGCCATTGCGGTCCTGGAGCACCCCCTGGCCCT
GGGGACTGGGGTTTGCAGCACCAGGGTGAAAGTGTTTAGCTCAAGTATGC
AGCTTGTCGGCCAAGTGGATACCTTTGGGCTGAGCCTCTACTTTCCCTCC
AAAATAACTGCCTCCGCTGTGACCTTTGATCACCAGGGAAATGTGATTGT
TGCAGATACATCTGGTCCAGCTATCCTTTGCTTAGGAAAACCTGAGGAGT
TTCCAGTACCGAAGCCCATGGTCACTCATGGTCTTTCGCATCCTGTGGCT
CTTACCTTCACCAAGGAGAATTCTCTTCTTGTGCTGGACACAGCATCTCA
TTCTATAAAAGTCTATAAAGTTGACTGGGGGTGAagatct

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAEASESGPALHELMREA
EISLLECKVCFEKFGHRQQRRPRNLSCGHVVCLACVAALAHPRTLALECP
FCRRACRGCDTSDCLPVLHLIELLGSALRQSPAAHRAAPSAPGALTCHHT
FGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGD
AAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPW
GVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAV
SWLTGAIAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSK
ITASAVTFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVAL
TFTKENSLLVLDTASHSIKVYKVDWG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET-Duet1-6His-TEV-laforin, GST-3C-Malin90.03 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65613
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No