Recombinant Protein - NEK3 (1 - 506)

Name: NEK3 (1 - 506)
Price per aliquot (100µg): £110.00
Long Name:
NIMA related kinase 3
Synonyms:
HSPK36, serine/threonine-protein kinase NEK3, phosphorylase B kinase kinase, MGC29949, glycogen synthase A kinase, NIMA (never in mitosis gene a)-related kinase 3
Catalog Number (DU Number):
DU 41229
Accession:
NM_002498.2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Enzymatic Substrate:
Myelin Basic Protein (MBP)
Calculated molecular mass:
Mono-Isotopic Mass: 84, 474.84 daltons
Average Mass: 84, 528.83 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical pI:
6.33
Native Sequence:
Amino acids M1 – R506 (end residue) of human NEK3. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228

Do you need any help? Please get in touch and we’ll be happy to lend a hand.