Recombinant Protein - PDGFRA (551 - 1089)

Name: PDGFRA (551 - 1089)
Price per aliquot (100µg): £110.00
Long Name:
platelet derived growth factor receptor alpha
Synonyms:
PDGFRA, PDGFR alpha, PDGFR2, CD140a, GAS9, platelet-derived growth factor receptor, alpha polypeptide, PDGFRAlpha
Catalog Number (DU Number):
DU63237
Accession:
NM_006206
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
KKKKEEIYFFFG
Calculated molecular mass:
Mono-Isotopic Mass: 88, 197.12 daltons
Average Mass: 88, 253.72 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
5.30
Native Sequence:
Amino acids Q551 – L1089 (end residue) of human PDGFRA. Residue Q232 of the fusion protein is equivalent to Q551 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1sites of pFastBac Dual.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.