Recombinant Protein - cyclin K (11 - 267)

Name: cyclin K (11 - 267)
Price per aliquot (100µg): £100.00
Synonyms:
CCNK
Catalog Number (DU Number):
DU 46325
Accession:
NM_001099402.1
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 56, 971.96 daltons
Average Mass: 57, 009.04 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
6.30
Native Sequence:
Amino acids S11 – H267 (end residue is R580) of human Cyclin K. Residue S232 of the fusion protein is equivalent to S11 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

Do you need any help? Please get in touch and we’ll be happy to lend a hand.