Recombinant Protein - VEGFR2 (789 - 1356)

Name: VEGFR2 (789 - 1356)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU26346
Accession:
NM_002253.2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
32P filter binding
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu:Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 90, 880.67 daltons
Average Mass: 90, 938.67 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.27
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids V789 – V1356 (end) of human VEGFR2. Residue V238 of the fusion protein is equivalent to V789 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission site (LEVLFQGP) residues 221 – 228
Cloning Sites:
Sal1 and Not1 sites of pFastBac GST 6P3

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